Detection of B7‑H2 by Western Blot.
Western blot shows lysate of U937 human histiocytic lymphoma cell line. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human B7‑H2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF165) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for B7‑H2 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of B7‑H2 in U937 Human Cell Line by Flow Cytometry.
U937 human histiocytic lymphoma cell line was stained with Goat Anti-Human B7‑H2 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF165, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Human B7-H2, also called B7RP-1, B7h, LICOS, and GL50, is a 60 kDa member of the B7 family of immune costimulatory proteins, which includes B7-1, B7-2, B7-H1 (PD-L1), PD-L2, and B7-H3. B7 proteins are members of the immunoglobulin (Ig) superfamily, the extracellular domains contain 2 Ig-like domains and all members have short cytoplasmic domains. Family members share about 20‑25% amino acid identity. Within the extracellular domain, human B7-H2 shares 49% and 54% amino acid sequence identity with human and rat B7-H2, respectively. B7-H2 has been identified as the ligand for ICOS, a member of the CD28 family of costimulatory receptors. Human B7-H2 is a 309 amino acid (aa) protein with a putative 18 aa signal peptide, a 239 aa extracellular domain, an 18 aa transmembrane region, and a 33 aa cytoplasmic domain. Human B7-H2 is expressed constitutively on resting B cells, dendritic cells, and at low levels on monocytes. The B7-H2/ICOS interaction appears to play roles in T cell dependent B cell activation and Th differentiation.
Coyle, A.J. and J.C. Gutierrez-Ramos (2001) Nat. Immunol. 2:203.
Ling, V. et al. (2000) J. Immunol. 164:1653.
Wang, S. et al. (2000) Blood 96:2808.
Brodie, D. et al. (2000) Curr. Biol. 10:333.
Mages, H.W. et al. (2000) Eur. J. Immunol. 30:1040.
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We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: Flow Cytometry.
Detection of B7-H2 in MCF-7. 106 cells were incubated with 0.50 ug of Goat anti-Human B7-H2 antibody (red), or Goat Isotype Control IgG (brown), followed by PE-conjugated anti-Goat secondary antibody (1:100). Unfixed cells.