Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Flow Cytometry, CyTOF-ready

Cited:

Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all B7-H2 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human B7-H2
Asp19-Ser258
Accession # O75144

Specificity

Detects human B7-H2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross‑reactivity with recombinant mouse B7-H2 and recombinant human B7-H1 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human B7‑H2 Antibody

Detection of B7-H2 antibody by Western Blot.

Detection of B7‑H2 by Western Blot.

Western blot shows lysate of U937 human histiocytic lymphoma cell line. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human B7-H2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF165) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for B7-H2 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of B7-H2 antibody in U937 Human Cell Line antibody by Flow Cytometry.

Detection of B7‑H2 in U937 Human Cell Line by Flow Cytometry.

U937 human histiocytic lymphoma cell line was stained with Goat Anti-Human B7-H2 Antigen Affinity-purified Polyclonal Anti-body (Catalog # AF165, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).

Applications for Human B7‑H2 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: U937 human histiocytic lymphoma cell line

Western Blot

2 µg/mL
Sample: U937 human histiocytic lymphoma cell line

Reviewed Applications

Read 1 review rated 5 using AF165 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

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  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: B7-H2

Human B7-H2, also called B7RP-1, B7h, LICOS, and GL50, is a 60 kDa member of the B7 family of immune costimulatory proteins, which includes B7-1, B7-2, B7-H1 (PD-L1), PD-L2, and B7-H3. B7 proteins are members of the immunoglobulin (Ig) superfamily, the extracellular domains contain 2 Ig-like domains and all members have short cytoplasmic domains. Family members share about 20‑25% amino acid identity. Within the extracellular domain, human B7-H2 shares 49% and 54% amino acid sequence identity with human and rat B7-H2, respectively. B7-H2 has been identified as the ligand for ICOS, a member of the CD28 family of costimulatory receptors. Human B7-H2 is a 309 amino acid (aa) protein with a putative 18 aa signal peptide, a 239 aa extracellular domain, an 18 aa transmembrane region, and a 33 aa cytoplasmic domain. Human B7-H2 is expressed constitutively on resting B cells, dendritic cells, and at low levels on monocytes. The B7-H2/ICOS interaction appears to play roles in T cell dependent B cell activation and Th differentiation.

References

  1. Coyle, A.J. and J.C. Gutierrez-Ramos (2001) Nat. Immunol. 2:203.
  2. Ling, V. et al. (2000) J. Immunol. 164:1653.
  3. Wang, S. et al. (2000) Blood 96:2808.
  4. Brodie, D. et al. (2000) Curr. Biol. 10:333.
  5. Mages, H.W. et al. (2000) Eur. J. Immunol. 30:1040.
  6. Swallow, M.M. et al. (1999) Immunity 11:423.
  7. Yoshinaga, S.K. et al. (1999) Nature 402:827.

Long Name

B7 Homolog 2

Alternate Names

B7H2, B7RP-1, CD275, GL50, ICOSL, ICOSLG

Entrez Gene IDs

23308 (Human); 50723 (Mouse); 499415 (Rat); 102132485 (Cynomolgus Monkey)

Gene Symbol

ICOSLG

UniProt

O75144

Additional B7-H2 Products

Product Documents for Human B7‑H2 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human B7‑H2 Antibody

For research use only

Citations for Human B7‑H2 Antibody

Customer Reviews for Human B7‑H2 Antibody (1)

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  • Human B7-H2 Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: MCF-7 human breast cancer cell line
    Species: Human
    Verified Customer | Posted 03/22/2017
    Detection of B7-H2 in MCF-7. 106 cells were incubated with 0.50 ug of Goat anti-Human B7-H2 antibody (red), or Goat Isotype Control IgG (brown), followed by PE-conjugated anti-Goat secondary antibody (1:100). Unfixed cells.
    Human B7‑H2 Antibody AF165

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