Human BAK Antibody

Catalog # Availability Size / Price Qty
MAB8161
MAB8161-SP
Detection of Human BAK by Western Blot.
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Human BAK Antibody Summary

Species Reactivity
Human
Specificity
Detects endogenous human BAK in Western blots.
Source
Monoclonal Mouse IgG1 Clone # 564305
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human BAK
Pro20-Asn124
Accession # Q16611
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunocytochemistry
5-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human BAK antibody by Western Blot. View Larger

Detection of Human BAK by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line, 293T human embryonic kidney epithelial cell line, and Daudi human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human BAK Monoclonal Antibody (Catalog # MAB8161) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for BAK at approximately 23 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Immunocytochemistry BAK antibody in Jurkat Human Cell Line by Immunocytochemistry (ICC). View Larger

BAK in Jurkat Human Cell Line. BAK was detected in immersion fixed Jurkat human acute T cell leukemia cell line using Mouse Anti-Human BAK Monoclonal Antibody (Catalog # MAB8161) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BAK

BAK (Bcl-2 homologous antagonist/killer; also know as BAK1 and Bcl-2-like protein 7) is a 25‑30 kDa member of the BCL-2 family of proteins. It is widely expressed and participates in the apoptotic cycle. BAK is an outer mitochondrial membrane protein that is inactive as a Zn-dependent homodimer. Upon activation by p53 or tBID, BAK oligomerizes, creating a pore in the mitochondrial membrane and allowing for cytochrome C release. Human BAK contains three Bcl-2 homology domains (aa 74‑88, 117‑136 and 169‑184), a Zn-binding region (aa 160‑166) and a C-terminal transmembrane segment (aa 188‑205). Amino acids 67‑94 mediate oligomerization of BAK. There are two potential isoform variants; one shows an alternate start site at Met 96, while a second shows a deletion of aa 46‑66. Over amino acids 20‑124, human BAK shares 76% aa identity with mouse BAK.

Long Name
Bcl-2 Antagonist/Killer
Entrez Gene IDs
578 (Human); 12018 (Mouse)
Alternate Names
Apoptosis regulator BAK; BAK; BAK1; BAK-LIKE; BAKMGC3887; BCL2-antagonist/killer 1; BCL2L7; bcl2-L-7; BCL2L7bcl-2 homologous antagonist/killer; BCL2-like 7 protein; Bcl-2-like protein 7; CDN1; MGC117255; pro-apoptotic protein BAK

Product Datasheets

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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Human BAK Antibody
By Anonymous on 08/15/2019
Application: ELISA Sample Tested: Cancer cell lysates Species: Human