Detection of Human Bax Minus C-Terminus by Western Blot. Western blot shows lysates of THP-1 human acute monocytic leukemia cell line. ??? membrane was probed with 4 µg/mL Goat Anti-Human Bax Minus C-Terminus Polyclonal Antibody (Catalog # AB820) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). For additional reference, recombinant ??? Bax (1 ng) was included. A specific band for Bax was detected at approximately ??? kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
B cell leukemia/lymphoma-2 associated X protein (Bax) is a pro-apoptotic member of the Bcl-2 family of proteins found in many cell types. Bax is a cytosolic protein that translocates to the mitochondria and participates in Cytochrome c release in response to apoptotic stimuli. Aberrant expression of Bcl-2 family members can inappropriately promote or prevent apoptosis. Increased expression of Bax is associated with the apoptotic loss of neurons in Parkinson's disease.
Bcl Associated X Protein
Entrez Gene IDs:
581 (Human); 12028 (Mouse); 24887 (Rat)
apoptosis regulator BAX; BCL2-associated X protein; Bcl2-L-4; BCL2L4bcl2-L-4; Bcl-2-like protein 4
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