Detection of Human Bcl‑10 by Western Blot. Western blot shows lysates of Raji human Burkitt's lymphoma cell line, A431 human epithelial carcinoma cell line, and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human Bcl‑10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2889) followed by HRP-conjugated Anti‑Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Bcl-10 at approximately 28 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 4.
Bcl‑10 in A431 Human Cell Line. Bcl‑10 was detected in immersion fixed A431 human epithelial carcinoma cell line using Goat Anti-Human Bcl‑10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2889) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
B cell CLL/leukemia 10 (Bcl-10), a 233 amino acid, 26.2 kDa protein, is the cellular homolog of the equine herpesvirus-2 gene (E10). Bcl-10 is also known as CARD like apoptotic protein (CLAP), CED-3/ICH-1 prodomain homologous E10-like regulator (CIPER), celluar-E10 (c-E10), and CARD-containing molecule enhancing NF kappa B (CARMEN). Bcl-10 contains a caspase recruitment domain (CARD) and has been shown to induce apoptosis and to activate NF kappa B. Bcl-10 can interact with other CARD domain containing proteins including CARD9, 10, 11, and 14, which function as upstream regulators of NF kappa B signaling. Defects in Bcl‑10 may be involved in the pathogenesis of human malignancy.
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