|Detection of Human Bcl‑2 Minus C-Terminus by Western Blot. Western blot shows lysates of KG‑1 human acute myelogenous leukemia cell line. PVDF membrane was probed with 0.1-0.5 µg/mL of Mouse Anti-Human Bcl‑2 Minus C-Terminus Monoclonal Antibody (Catalog # MAB827) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Bcl‑2 Minus C-Terminus at approximately 24 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.|
|Detection of Human Bcl‑2 by Simple WesternTM. Simple Western lane view shows lysates of MCF‑7 human breast cancer cell line, loaded at 0.5 mg/mL. A specific band was detected for Bcl‑2 at approximately 24 kDa (as indicated) using 5 µg/mL of Mouse Anti-Human Bcl‑2 Minus C-Terminus Monoclonal Antibody (Catalog # MAB827). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.|
Bcl-2 is a member of a family of proteins that regulates outer mitochondrial membrane permeability (1, 2). Bcl-2 is an anti-apoptotic member that prevents release of cytochrome c from the mitochondria intermembrane space into the cytosol. Bcl-2 is present on the outer mitochondrial membrane and is also found on other membranes in some cell types. Natural Bcl-2 contains a carboxyl-terminal mitochondria targeting sequence. Recombinant Bcl-2, missing the mitochondrial targeting sequence, maintains its ability to neutralize pro-apoptotic Bcl-2 family members. Neutralization by Bcl-2 appears to be through binding the BH3 region of pro-apoptotic Bcl-2 family members. This activity does not require the mitochondrial targeting sequence.
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