Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 34301
View all Betacellulin/BTC Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Betacellulin/BTC
Asp32-Tyr111
Accession # P35070

Specificity

Detects human Betacellulin/BTC in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse (rm) BTC, recombinant human (rh) Amphiregulin, rhEGF, rhHB-EGF, rhHeregulin-alpha, rhHeregulin-beta, rhTGF-alpha, or rmEpiregulin is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Betacellulin/BTC Antibody

Betacellulin/BTC antibody in Human Pancreas by Immunohistochemistry (IHC-P).

Betacellulin/BTC in Human Pancreas.

Betacellulin/BTC was detected in formalin fixed paraffin-embedded sections of human pancreas using Mouse Anti-Human Betacellulin/BTC Monoclonal Antibody (Catalog # MAB2611) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counter-stained with hematoxylin (blue). Specific staining was localized to cells of the Islet of Langerhans. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human Betacellulin/BTC Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human pancreas

Western Blot

1 µg/mL
Sample: Recombinant Human Betacellulin/BTC (Catalog # 261-CE)

Reviewed Applications

Read 1 review rated 5 using MAB2611 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Betacellulin/BTC

Betacellulin (BTC) is a new member of the EGF family of cytokines that is comprised of at least ten proteins including EGF, TGF-alpha, amphiregulin, HB-EGF, and the various heregulins. All of these cytokines are synthesized as transmembrane precursors and are characterized by the presence of one or more EGF structural units in their extracellular domain. The soluble forms of these cytokines are released by proteolytic cleavage. BTC, a heparin-binding protein, was originally isolated from the conditioned media of mouse pancreatic beta tumor cells as a 32 kDa glycoprotein composed of 80 amino acid residues. The cDNA encoding human BTC was cloned from a human breast adenocarcinoma cell line (MCF-7) cDNA library. Human and mouse cDNAs encode BTC precursor proteins of 178 and 177 amino acid residues, respectively. At the amino acid sequence level, human BTC precursor protein exhibits 79% identity with that of the mouse BTC precursor. In a mouse cell line transfected with human BTC cDNA, three forms of soluble human BTC have been detected: the glycosylated, intact BTC composed of 80 amino acid residues, a truncated molecule lacking 12 amino acid residues from the amino terminus, and a second truncated molecule lacking 30 amino acid residues from the amino terminus. The biological activities of these BTC forms were shown to be identical. BTC can bind to the EGF receptor and is a potent mitogen for Balb/c 3T3 fibroblasts, retinal pigment epithelial cells and vascular smooth muscle cells.

Alternate Names

BTC

Entrez Gene IDs

685 (Human); 12223 (Mouse)

Gene Symbol

BTC

UniProt

P35070

Additional Betacellulin/BTC Products

Product Documents for Human Betacellulin/BTC Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Betacellulin/BTC Antibody

For research use only

Related Research Areas

EGF Family

Citations for Human Betacellulin/BTC Antibody

Customer Reviews for Human Betacellulin/BTC Antibody (1)

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  • Human Betacellulin/BTC Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Pancreas tissue
    Species: Human
    Verified Customer | Posted 01/20/2022
    Human Betacellulin/BTC Antibody MAB2611

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