Human Betacellulin/BTC Antibody

Catalog # Availability Size / Price Qty
MAB261-SP
MAB261-500
MAB261-100
Product Details
Citations (1)
FAQs
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Human Betacellulin/BTC Antibody Summary

Species Reactivity
Human
Specificity
Detects human Betacellulin in ELISAs and Western blots. In Western blots, this antibody does not cross-react with recombinant human (rh) EGF, rhHB‑EGF, rhTGF‑ alpha, rhHRG‑ alpha, or rhAR under non-reducing conditions.
Source
Monoclonal Mouse IgG1 Clone # 34362
Purification
Protein A or G purified from ascites
Immunogen
E. coli-derived recombinant human Betacellulin
Asp32-Tyr111
Accession # P35070
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample

Human Betacellulin/BTC Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Human Betacellulin/BTC Biotinylated Antibody (Catalog # BAF261)

Standard: Recombinant Human Betacellulin Protein (Catalog # 261-CE)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Betacellulin/BTC

Betacellulin (BTC) is a new member of the EGF family of cytokines that is comprised of at least ten proteins including EGF, TGF-alpha, amphiregulin, HB-EGF, and the various heregulins. All of these cytokines are synthesized as transmembrane precursors and are characterized by the presence of one or more EGF structural units in their extracellular domain. The soluble forms of these cytokines are released by proteolytic cleavage. BTC, a heparin-binding protein, was originally isolated from the conditioned media of mouse pancreatic beta tumor cells as a 32 kDa glycoprotein composed of 80 amino acid residues. The cDNA encoding human BTC was cloned from a human breast adenocarcinoma cell line (MCF-7) cDNA library. Human and mouse cDNAs encode BTC precursor proteins of 178 and 177 amino acid residues, respectively. At the amino acid sequence level, human BTC precursor protein exhibits 79% identity with that of the mouse BTC precursor. In a mouse cell line transfected with human BTC cDNA, three forms of soluble human BTC have been detected: the glycosylated, intact BTC composed of 80 amino acid residues, a truncated molecule lacking 12 amino acid residues from the amino terminus, and a second truncated molecule lacking 30 amino acid residues from the amino terminus. The biological activities of these BTC forms were shown to be identical. BTC can bind to the EGF receptor and is a potent mitogen for Balb/c 3T3 fibroblasts, retinal pigment epithelial cells, and vascular smooth muscle cells.

Entrez Gene IDs
685 (Human); 12223 (Mouse)
Alternate Names
Betacellulin; BTC; probetacellulin

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Citation for Human Betacellulin/BTC Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Hydrogen peroxide and endothelin-1 are novel activators of betacellulin ectodomain shedding.
    Authors: Sanderson MP, Abbott CA, Tada H, Seno M, Dempsey PJ, Dunbar AJ
    J. Cell. Biochem., 2006-10-01;99(2):609-23.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development

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