Human Betacellulin/BTC Antibody
Human Betacellulin/BTC Antibody Summary
Accession # P35070
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human Betacellulin/BTC by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line and OVCAR-3 human ovarian carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human Betacellulin/BTC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-261-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Betacellulin/BTC at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Betacellulin/BTC in Human Lung Cancer Tissue. Betacellulin/BTC was detected in immersion fixed paraffin-embedded sections of human lung cancer tissue using Goat Anti-Human Betacellulin/BTC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-261-NA) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Betacellulin/BTC in Human Pancreas. Betacellulin/BTC was detected in immersion fixed paraffin-embedded sections of human pancreas using Goat Anti-Human Betacellulin/BTC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-261-NA) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to islets. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Cell Proliferation Induced by Betacellulin/BTC and Neutralization by Human Betacellulin/BTC Antibody. Recombinant Human Betacellulin/BTC (Catalog # 261-CE) stimulates proliferation in the Balb/3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human Betacellulin/BTC (1 ng/mL) is neutralized (green line) by increasing concen-trations of Goat Anti-Human Betacellulin/ BTC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-261-NA). The ND50 is typically 0.04-0.08 µg/mL.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Betacellulin (BTC) is a member of the EGF family of cytokines that is comprised of at least ten proteins including EGF, TGF-alpha, amphiregulin, HB-EGF, and the various heregulins. All of these cytokines are synthesized as transmembrane precursors and are characterized by the presence of one or more EGF structural units in their extracellular domain. The soluble forms of these cytokines are released by proteolytic cleavage. BTC, a heparin-binding protein, was originally isolated from the conditioned media of mouse pancreatic beta tumor cells as a 32 kDa glycoprotein composed of 80 amino acid residues. The cDNA encoding human BTC was cloned from a human breast adenocarcinoma cell line (MCF-7) cDNA library. Human and mouse cDNAs encode BTC precursor proteins of 178 and 177 amino acid residues, respectively. At the amino acid sequence level, human BTC precursor protein exhibits 79% identity with that of the mouse BTC precursor. In a mouse cell line transfected with human BTC cDNA, three forms of soluble human BTC have been detected: the glycosylated, intact BTC composed of 80 amino acid residues, a truncated molecule lacking 12 amino acid residues from the amino terminus, and a second truncated molecule lacking 30 amino acid residues from the amino terminus. The biological activities of these BTC forms were shown to be identical. BTC can bind to the EGF receptor and is a potent mitogen for Balb/c 3T3 fibroblasts, retinal pigment epithelial cells and vascular smooth muscle cells.
Citations for Human Betacellulin/BTC Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 6
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Locally advanced rectal cancer transcriptomic-based secretome analysis reveals novel biomarkers useful to identify patients according to neoadjuvant chemoradiotherapy response
Authors: LMD Canto, SS Cury, MC Barros-Fil, BEC Kupper, MDFS Begnami, C Scapulatem, RF Carvalho, FA Marchi, DA Olsen, JS Madsen, BM Havelund, S Aguiar, SR Rogatto
Sci Rep, 2019-06-18;9(1):8702.
Sample Types: Serum
Applications: ELISA Capture
HER family receptor abnormalities in lung cancer brain metastases and corresponding primary tumors.
Authors: Sun M, Behrens C, Feng L, Ozburn N, Tang X, Yin G, Komaki R, Varella-Garcia M, Hong WK, Aldape KD, Wistuba II
Clin. Cancer Res., 2009-07-21;15(15):4829-37.
Sample Types: Whole Tissue
High cortical bone mass phenotype in betacellulin transgenic mice is EGFR dependent.
Authors: Schneider MR, Mayer-Roenne B, Dahlhoff M, Proell V, Weber K, Wolf E, Erben RG
J. Bone Miner. Res., 2009-03-01;24(3):455-67.
Sample Types: Cell Lysates, Whole Tissue
Applications: IHC, Western Blot
Betacellulin stimulates growth of the mouse intestinal epithelium and increases adenoma multiplicity in Apc+/Min mice.
Authors: Dahlhoff M, Horst D, Gerhard M, Kolligs FT, Wolf E, Schneider MR
FEBS Lett., 2008-07-24;582(19):2911-5.
Sample Types: Whole Tissue
Autocrine extracellular signal-regulated kinase (ERK) activation in normal human keratinocytes: metalloproteinase-mediated release of amphiregulin triggers signaling from ErbB1 to ERK.
Authors: Kansra S, Johnson JL
Mol. Biol. Cell, 2004-07-14;15(9):4299-309.
Sample Types: Whole Cells
Changes in the expression and cytological localization of betacellulin and its receptors (ErbB-1 and ErbB-4) in the trophoblasts in human placenta over the course of pregnancy.
Authors: Tanimura K, Nakago S, Murakoshi H, Takekida S, Moriyama T, Matsuo H, Hashimoto K, Maruo T
Eur. J. Endocrinol., 2004-07-01;151(1):93-101.
Sample Types: Tissue Homogenates
Applications: IHC-Fr, Western Blot
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