Human BMPR-II Antibody

(6 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human BMPR-II in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) BMPR‑IA, recombinant mouse (rm) BMPR-IA, rhBMPR-IB, and rmBMPR-IB is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human BMPR-II
    Ala26-Ile151
    Accession # Q13873
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Human BMPR-II Fc Chimera (Catalog # 811-BR)
  • Flow Cytometry
    2.5 µg/106 cells
    See below
  • Immunohistochemistry
    5-15 µg/mL
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
BMPR-II in Human Prostate. BMPR-II was detected in formalin fixed paraffin-embedded sections of human prostate using Goat Anti-Human BMPR-II Antigen Affinity-purified Polyclonal Antibody (Catalog # AF811) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the plasma membrane of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of BMPR‑II in PC‑3 Human Cell Line by Flow Cytometry. PC‑3 human prostate cancer cell line was stained with Goat Anti-Human BMPR‑II Antigen Affinity-purified Polyclonal Antibody (Catalog # AF811, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: BMPR-II

Like other members of the TGF-beta superfamily, cellular responses to bone morphogenetic proteins (BMPs) have been shown to be mediated by the binding to heteromeric complex of type I and type II serine-threonine kinase receptors. Both receptor types are required for the signal transduction. BMP receptor II (BMPR-II) is one of the five mammalian type II receptors (including TGF-beta R-II, ActR-II, ActR-IIB, BMPR-II, and MISR-II) for the TGF-beta superfamily ligands. The type II receptors for TGF-beta and activin bind ligands with high affinity by itself. In contrast, BMPR-II binds BMP-2, BMP-4, and BMP-7 weakly in the absence of type I receptor, and the binding can be facilitated by the presence of the type I receptor. BMPR-II mRNA is widely expressed in fetal and adult tissues. Human and mouse BMPR-II are highly conserved sharing 97% sequence identity (1-3).

  • References:
    1. Rosenzweig, B.L. et al. (1995) Proc. Natl. Acad. Sci. USA 92:7632.
    2. Beppu, H. et al. (1997) Biochem. Biophys. Res. Commun. 235:499.
    3. Kawabata, M. et al. (1998) Cytokine and Growth Factor Reviews 9:49.
  • Long Name:
    Bone Morphogenetic Protein Receptor II
  • Entrez Gene IDs:
    659 (Human); 12168 (Mouse)
  • Alternate Names:
    BMP type II receptor; BMP type-2 receptor; BMPR2; BMPR-2; BMPR3; BMPRII; BMPR-II; BMPR-IIprimary pulmonary hypertension 1; Bone morphogenetic protein receptor type II; bone morphogenetic protein receptor type-2; bone morphogenetic protein receptor, type II (serine/threonine kinase); BRK-3; EC 2.7.11; EC 2.7.11.30; FLJ41585; FLJ76945; PPH1BMR2; T-ALK; type II activin receptor-like kinase; type II receptor for bone morphogenetic protein-4
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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Species
Applications
Sample Type
  1. Bone morphogenetic protein-9 activates Smad and ERK pathways and supports human osteoclast function and survival in vitro.
    Authors: Fong D, Bisson M, Laberge G, McManus S, Grenier G, Faucheux N, Roux S
    Cell Signal, 2013;25(4):717-28.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  2. Epigenetic repression of bone morphogenetic protein receptor II expression in scleroderma.
    Authors: Wang Y, Kahaleh B
    J Cell Mol Med, 2013;17(10):1291-9.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  3. BMP4 is a peripherally-derived factor for motor neurons and attenuates glutamate-induced excitotoxicity in vitro.
    Authors: Chou H, Lai D, Huang C, McLennan I, Wang H, Wang P
    PLoS ONE, 2013;8(3):e58441.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  4. Bone morphogenetic protein receptor IB as a marker for enrichment of osteogenic precursor-like cells in human dermis.
    Authors: He J, Dong J, Wang T
    Arch. Dermatol. Res., 2011;303(8):581-90.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  5. High-density lipoproteins affect endothelial BMP-signaling by modulating expression of the activin-like kinase receptor 1 and 2.
    Authors: Yao Y, Shao ES, Jumabay M, Shahbazian A, Ji S, Bostrom KI
    Arterioscler. Thromb. Vasc. Biol., 2008;28(12):2266-74.
    Species: Human
    Sample Type: Whole Cells
    Application: WB
  6. Cell origin in experimental repair of cricoid cartilage defects treated with recombinant human bone morphogenetic protein-2.
    Authors: Tcacencu I, Carlsoo B, Stierna P
    Wound Repair Regen, 2005;13(3):341-9.
    Species: Rabbit
    Sample Type: Whole Tissue
    Application: IHC
Cell and Tissue Staining Kits
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Normal Goat IgG Control

Ctrl AB-108-C 191  
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Secondary Antibodies
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Goat IgG HRP-conjugated Antibody

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Goat IgG (H+L) APC-conjugated Antibody

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Goat IgG Horseradish Peroxidase-conjugated Antibody

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Donkey Anti-Goat IgG Antibody

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Goat IgG (H+L) Fluorescein-conjugated Antibody

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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

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Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

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Chicken Anti-Goat IgG Biotinylated Antibody

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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

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