Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all BNIP3 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human BNIP3
Ser2-Glu160
Accession # Q12983

Specificity

Detects human BNIP3 in Western blots. In Western blots, this antibody shows no cross-reactivity with recombinant human BNIP3L.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human BNIP3 Antibody

Detection of Human BNIP3 antibody by Western Blot.

Detection of Human BNIP3 by Western Blot.

Western blot shows lysates of A549 human lung carcinoma cell line and MCF-7 human breast cancer cell line untreated (-) or treated (+) with 150 µM CoCl2for 16 hours. PVDF membrane was probed with 1 µg/mL of Human BNIP3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4147), followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for BNIP3 at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Detection of BNIP3 by Western Blot

Detection of BNIP3 by Western Blot

The hypoxic pathway in glioblastoma tumours. Levels of 7 HIF-pathway members were estimated by Western blotting (A), with densitometry measures (B), or measured by ELISA (C). A HIF-pathway score was derived for each patient by combining the relative scores of 7 hypoxia-responsive proteins (D); IDH1 mutant samples are shown as solid square symbols. n = 37 samples; T, tumour, +, positive control (MDA-MB-231 cell line exposed to 1% O2 for 16 h), mw, molecular weight marker; mean ± SEM. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35419292), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human BNIP3 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: CoCl2-treated A549 human lung carcinoma cell line and MCF‑7 human breast cancer cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: BNIP3

Bcl-2/adenovirus E1B 19 kDa protein-interacting protein 3 (BNIP3), also known as 19 kDa interacting protein 3 (NIP3), is a proapoptotic member of Bcl-2 protein family. BNIP3 is a 194 amino acid, 21.5 kDa (predicted) protein that contains a single Bcl-2 homology 3 (BH3) domain and a C-terminal transmembrane domain required for mitochondrial localization, homodimerization, and regulation of its proapoptotic function. BNIP3 was identified as one of several proteins that interact with discrete domains of Bcl-2 and the E1B 19 kDa protein. Under conditions of prolonged oxygen deprivation, the hypoxia-induced protein HIF1-alpha activates expression of BNIP3, which in turn, promotes apoptosis under these conditions. The mechanism of BNIP3-mediated apoptosis is independent of caspase activation and cytochrome c release and is characterized by early plasma membrane and mitochondrial damage, prior to the appearance of chromatin condensation or DNA fragmentation. Human BNIP3 shares 90% amino acid identity with mouse and rat BNIP3. Human BNIP3 shares 56% amino acid sequence identity with human BNIP3L.

Long Name

BCL2/Adenovirus E1B 19 Kda Protein-Interacting Protein 3-Like

Alternate Names

Nip3

Entrez Gene IDs

664 (Human); 12176 (Mouse); 84480 (Rat)

Gene Symbol

BNIP3

UniProt

Q12983

Additional BNIP3 Products

Product Documents for Human BNIP3 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human BNIP3 Antibody

For research use only

Related Research Areas

Autophagy
Bcl-2 Proteins and Regulators

Citations for Human BNIP3 Antibody

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