|Detection of Human BNIP3 by Western Blot. Western blot shows lysates of A549 human lung carcinoma cell line and MCF‑7 human breast cancer cell line untreated (-) or treated (+) with 150 μM CoCl2 for 16 hours. PVDF membrane was probed with 1 µg/mL of Human BNIP3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4147), followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for BNIP3 at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.|
Bcl-2/adenovirus E1B 19 kDa protein-interacting protein 3 (BNIP3), also known as 19 kDa interacting protein 3 (NIP3), is a proapoptotic member of Bcl-2 protein family. BNIP3 is a 194 amino acid, 21.5 kDa (predicted) protein that contains a single Bcl-2 homology 3 (BH3) domain and a C-terminal transmembrane domain required for mitochondrial localization, homodimerization, and regulation of its proapoptotic function. BNIP3 was identified as one of several proteins that interact with discrete domains of Bcl-2 and the E1B 19 kDa protein. Under conditions of prolonged oxygen deprivation, the hypoxia-induced protein HIF1-alpha activates expression of BNIP3, which in turn, promotes apoptosis under these conditions. The mechanism of BNIP3-mediated apoptosis is independent of caspase activation and cytochrome c release and is characterized by early plasma membrane and mitochondrial damage, prior to the appearance of chromatin condensation or DNA fragmentation. Human BNIP3 shares 90% amino acid identity with mouse and rat BNIP3. Human BNIP3 shares 56% amino acid sequence identity with human BNIP3L.
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