Human BNIP3 Antibody

Catalog #: AF4147
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF4147
AF4147-SP

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Detection of Human BNIP3 by Western Blot.

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Citations (1)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human BNIP3 Antibody Summary

Species Reactivity

Human

Specificity

Detects human BNIP3 in Western blots. In Western blots, this antibody shows no cross-reactivity with recombinant human BNIP3L.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human BNIP3
Ser2-Glu160
Accession # Q12983

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human BNIP3 by Western Blot. Western blot shows lysates of A549 human lung carcinoma cell line and MCF-7 human breast cancer cell line untreated (-) or treated (+) with 150 µM CoCl₂for 16 hours. PVDF membrane was probed with 1 µg/mL of Human BNIP3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4147), followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for BNIP3 at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BNIP3

Bcl-2/adenovirus E1B 19 kDa protein-interacting protein 3 (BNIP3), also known as 19 kDa interacting protein 3 (NIP3), is a proapoptotic member of Bcl-2 protein family. BNIP3 is a 194 amino acid, 21.5 kDa (predicted) protein that contains a single Bcl-2 homology 3 (BH3) domain and a C-terminal transmembrane domain required for mitochondrial localization, homodimerization, and regulation of its proapoptotic function. BNIP3 was identified as one of several proteins that interact with discrete domains of Bcl-2 and the E1B 19 kDa protein. Under conditions of prolonged oxygen deprivation, the hypoxia-induced protein HIF1-alpha activates expression of BNIP3, which in turn, promotes apoptosis under these conditions. The mechanism of BNIP3-mediated apoptosis is independent of caspase activation and cytochrome c release and is characterized by early plasma membrane and mitochondrial damage, prior to the appearance of chromatin condensation or DNA fragmentation. Human BNIP3 shares 90% amino acid identity with mouse and rat BNIP3. Human BNIP3 shares 56% amino acid sequence identity with human BNIP3L.

Long Name

BCL2/Adenovirus E1B 19 Kda Protein-Interacting Protein 3-Like

Entrez Gene IDs

664 (Human); 12176 (Mouse); 84480 (Rat)

Alternate Names

BCL2/adenovirus E1B 19 kDa protein-interacting protein 3; BCL2/adenovirus E1B 19kDa interacting protein 3; BCL2/adenovirus E1B 19kD-interacting protein 3; BNIP3; Nip3

Product Datasheets

Product Datasheet

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Related Research Areas

Citation for Human BNIP3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Expression and Functional Characterization of the BNIP3 Protein in Renal Cell Carcinomas
Authors: S Macher-Goe, M Keith, G Hatiboglu, M Hohenfelln, P Schirmache, W Roth, KE Tagscherer
Transl Oncol, 2017;10(6):869-875.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot