Detection of Human BRIX by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human BRIX Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4024) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for BRIX at approximately 41 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
BRIX (Biogenesis of ribosomes in Xenopus; also BRIX domain-containing protein 2) is a 41 kDa (predicted) member of the BXDC2 family of proteins. It is found in nucleoli, and appears to participate in the formation of a complex associated with pre-rRNA and enzymes that generate 60S ribosomal subunits. Human BRIX is 353 amino acids (aa) in length. It is characterized by the presence of a BRIX domain that lies between aa 60‑249. There is one splice variant that shows a 17 aa substitution for aa 11‑27. Over aa 131‑353, human BRIX shares 93% aa identity with mouse BRIX.
Brix Domain Containing 2
Entrez Gene IDs:
55299 (Human); 67832 (Mouse); 294799 (Rat)
brix domain containing 2; Brix domain-containing protein 2; BRIX; BRIX1; BRIXBXDC2; BRX1, biogenesis of ribosomes, homolog (S. cerevisiae); BXDC2; FLJ11100; ribosome biogenesis protein BRX1 homolog
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