Human BTK Antibody

Catalog # Availability Size / Price Qty
Detection of Human BTK by Western Blot.
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Human BTK Antibody Summary

Species Reactivity
Detects endogenous human BTK in Western blots.
Monoclonal Mouse IgG1 Clone # 566928
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human BTK
Accession # Q06187
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
0.05 µg/mL
See below
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human BTK antibody by Western Blot. View Larger

Detection of Human BTK by Western Blot. Western blot shows lysates of Raji human Burkitt's lymphoma cell line and Daudi human Burkitt's lymphoma cell line (BTK positive) and Jurkat human acute T cell leukemia cell line (BTK negative). PVDF membrane was probed with 0.05 µg/mL of Human BTK Monoclonal Antibody (Catalog # MAB5807) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for BTK at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry BTK antibody in Raji Human Cell Line by Immunocytochemistry (ICC). View Larger

BTK in Raji Human Cell Line. BTK was detected in immersion fixed Raji human Burkitt's lymphoma cell line using Mouse Anti-Human BTK Monoclonal Antibody (Catalog # MAB5807) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BTK

Bruton tyrosine kinase (BTK) is a 75 kDa cytoplasmic protein tyrosine kinase that is widely expressed in hematopoietic cells. BTK is required for B cell receptor signaling and B cell development. Defects in BTK result in X-linked agammaglobulinemia which is characterized by a severely decreased level of circulating antibodies. Like other Tec family kinases, BTK contains a Pleckstrin homology domain, a Tec homology domain, an SH3 domain, an SH2 domain, and a protein kinase domain. Association of the BTK SH2 domain with the B cell linker protein (BLNK) is required for the activation of PLC gamma by BTK. Within the SH2 domain (aa 280‑377), human BTK shares 99% aa sequence identity with mouse and rat BTK.

Long Name
Bruton Agammaglobulinemia Tyrosine Kinase
Entrez Gene IDs
695 (Human)
Alternate Names
Agammaglobulinaemia tyrosine kinase; AGMX1; AGMX1MGC126262; AT; ATKIMD1; B-cell progenitor kinase; BPK; Bruton agammaglobulinemia tyrosine kinase; Bruton tyrosine kinase; BTK; dominant-negative kinase-deficient Brutons tyrosine kinase; EC 2.7.10; EC; IMD1; PSCTK1; tyrosine-protein kinase BTK; XLA; XLAMGC126261

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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Human BTK Antibody
By Anonymous on 07/07/2022
Application: ICC/IF Sample Tested: Jurkat human acute T cell leukemia cell line Species: Human