Key Product Details

Species Reactivity

Human

Applications

Western Blot, Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all BTLA Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human BTLA
Ile25-Ser150
Accession # AAP44003

Specificity

Detects human BTLA in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human BTLA Antibody

Detection of Human BTLA antibody by Western Blot.

Detection of Human BTLA by Western Blot.

Western blot shows lysates of HEK293T human embryonic kidney cell line either mock transfected or transfected with human BTLA. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human BTLA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3354) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for BTLA at approximately 70-80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human BTLA Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: Human whole blood lymphocytes

Western Blot

0.5 µg/mL
Sample: HEK293T human embryonic kidney cell line transfected with human BTLA

Reviewed Applications

Read 1 review rated 5 using AF3354 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: BTLA

B and T Lymphocyte Attenuator (BTLA) also known as CD272, is a 70kDa type I transmembrane glycoprotein of the CD28 family of T cell costimulatory molecules. BTLA functions as a coinhibitor on activated T cells and on B cells and dendritic cells. The extracellular domain (ECD) of this Ig superfamily protein contains one
V‑type Ig-like domain, which interacts with the herpesvirus entry mediator (HVEM/TNFSF14). The amino acid sequence of the ECD of human BTLA shares only 34% identity to that of mouse BTLA. One human BTLA variant lacking the transmembrane sequence has been reported.

Long Name

B- And T-Lymphocyte Attenuator

Alternate Names

CD272

Entrez Gene IDs

151888 (Human); 208154 (Mouse); 102144877 (Cynomolgus Monkey)

Gene Symbol

BTLA

UniProt

AAP44003

Additional BTLA Products

Product Documents for Human BTLA Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human BTLA Antibody

For research use only

Citations for Human BTLA Antibody

Customer Reviews for Human BTLA Antibody (1)

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  • Human BTLA Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: NS0 mouse myeloma cell line
    Species: Human
    Verified Customer | Posted 12/20/2017
    Observed binding via ELISA to R&D huBTLA-hIgG1 (8385-BT). More efficient binding observed when compared to Biolegend and Thermo anti-huBTLA antibodies.
    Human BTLA Antibody AF3354

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