Detection of Human BTLA by Western Blot.
Western blot shows lysates of HEK293T human embryonic kidney cell line either mock transfected or transfected with human BTLA. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human BTLA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3354) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for BTLA at approximately
70-80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
B and T Lymphocyte Attenuator (BTLA) also known as CD272, is a 70kDa type I transmembrane glycoprotein of the CD28 family of T cell costimulatory molecules. BTLA functions as a coinhibitor on activated T cells and on B cells and dendritic cells. The extracellular domain (ECD) of this Ig superfamily protein contains one
V‑type Ig-like domain, which interacts with the herpesvirus entry mediator (HVEM/TNFSF14). The amino acid sequence of the ECD of human BTLA shares only 34% identity to that of mouse BTLA. One human BTLA variant lacking the transmembrane sequence has been reported.
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ELISA: Human BTLA Antibody [AF3354].
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