Detects human BTN2A2/Butyrophilin 2A2 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse BTN2A2 is observed, less than 5% cross-reactivity with recombinant human (rh) BTN2A1 and rhBTN3A2 is observed, and less than 1% cross-reactivity with rhBTN1A1 is observed.
Polyclonal Rabbit IgG
E. coli-derived recombinant human BTN2A2/Butyrophilin 2A2 Phe34-Val237 Accession # Q8WVV5
Supplied as a solution in PBS containing BSA, Glycerol and Sodium Azide *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Detection of Human BTN2A2/Butyrophilin 2A2 by Western Blot.
Western blot shows lysates of human kidney tissue, human heart tissue, and human testis tissue. PVDF membrane was probed with a 1:1000 dilution of Rabbit Anti-Human BTN2A2/Butyrophilin 2A2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8645) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for BTN2A2/Butyrophilin 2A2 at approximately 50-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
BTN2A2/Butyrophilin 2A2 in Human Thymus.
BTN2A2/Butyrophilin 2A2 was detected in immersion fixed paraffin-embedded sections of human thymus using Rabbit Anti-Human BTN2A2/Butyrophilin 2A2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8645) at a 1:50 dilution overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in endothelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Human BTN2A2/Butyrophilin 2A2 by Simple WesternTM.
Simple Western lane view shows lysates of human kidney tissue, human heart tissue, and human testis tissue, loaded at 0.2 mg/mL. A specific band was detected for BTN2A2/Butyrophilin 2A2 at approximately 60 kDa (as indicated) using 1:25 dilution of Rabbit Anti-Human BTN2A2/Butyrophilin 2A2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8645). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C, as supplied.
1 month, 2 to 8 °C under sterile conditions after opening.
6 months, -20 to -70 °C under sterile conditions after opening.
Background: BTN2A2/Butyrophilin 2A2
Butyrophilin subfamily 2 member A2 (BTN2A2) is a protein encoded by the BTN2A2 gene and is the major protein associated with fat droplets in the
milk. This gene is a member of the BTN2 subfamily of genes, which
encode proteins belonging to the butyrophilin protein family. The gene
is located in a cluster on chromosome 6, consisting of seven genes
belonging to the expanding B7/butyrophilin-like group, a subset of the
immunoglobulin gene superfamily. The encoded protein is a type 1
receptor glycoprotein involved in lipid, fatty-acid and sterol
metabolism. Several alternatively spliced transcript variants of this
gene have been described.
Butyrophilin Subfamily 2 Member A2
Entrez Gene IDs:
10385 (Human); 238555 (Mouse); 306957 (Rat)
BTF2; BTF2BT2.2butyrophilin 2; BTN2; BTN2A2; Butyrophilin 2; butyrophilin subfamily 2 member A2; butyrophilin, subfamily 2, member A2; FLJ41908
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