Butyrophilin subfamily 2 member A2 (BTN2A2) is a protein encoded by the BTN2A2 gene and is the major protein associated with fat droplets in the
milk. This gene is a member of the BTN2 subfamily of genes, which
encode proteins belonging to the butyrophilin protein family. The gene
is located in a cluster on chromosome 6, consisting of seven genes
belonging to the expanding B7/butyrophilin-like group, a subset of the
immunoglobulin gene superfamily. The encoded protein is a type 1
receptor glycoprotein involved in lipid, fatty-acid and sterol
metabolism. Several alternatively spliced transcript variants of this
gene have been described.
Human BTN2A2/Butyrophilin 2A2 Antibody
R&D Systems | Catalog # AF8645
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Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Western Blot, Simple Western
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
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Product Specifications
Immunogen
E. coli-derived recombinant human BTN2A2/Butyrophilin 2A2
Phe34-Val237
Accession # Q8WVV5
Phe34-Val237
Accession # Q8WVV5
Specificity
Detects human BTN2A2/Butyrophilin 2A2 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse BTN2A2 is observed, less than 5% cross-reactivity with recombinant human (rh) BTN2A1 and rhBTN3A2 is observed, and less than 1% cross-reactivity with rhBTN1A1 is observed.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Human BTN2A2/Butyrophilin 2A2 Antibody
Detection of Human BTN2A2/Butyrophilin 2A2 by Western Blot.
Western blot shows lysates of human kidney tissue, human heart tissue, and human testis tissue. PVDF membrane was probed with a 1:1000 dilution of Rabbit Anti-Human BTN2A2/Butyrophilin 2A2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8645) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for BTN2A2/Butyrophilin 2A2 at approximately 50-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
BTN2A2/Butyrophilin 2A2 in Human Thymus.
BTN2A2/Butyrophilin 2A2 was detected in immersion fixed paraffin-embedded sections of human thymus using Rabbit Anti-Human BTN2A2/Butyrophilin 2A2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8645) at a 1:50 dilution overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in endothelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Human BTN2A2/Butyrophilin 2A2 by Simple WesternTM.
Simple Western lane view shows lysates of human kidney tissue, human heart tissue, and human testis tissue, loaded at 0.2 mg/mL. A specific band was detected for BTN2A2/Butyrophilin 2A2 at approximately 60 kDa (as indicated) using 1:25 dilution of Rabbit Anti-Human BTN2A2/Butyrophilin 2A2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8645). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human BTN2A2/Butyrophilin 2A2 Antibody
Application
Recommended Usage
Immunohistochemistry
1:50 dilution
Sample: Immersion fixed paraffin-embedded sections of human thymus
Sample: Immersion fixed paraffin-embedded sections of human thymus
Simple Western
1:25 dilution
Sample: Human kidney tissue, Human heart tissue, and Human testis tissue
Sample: Human kidney tissue, Human heart tissue, and Human testis tissue
Western Blot
1:1000 dilution
Sample: human kidney tissue, human heart tissue, and human testis tissue
Sample: human kidney tissue, human heart tissue, and human testis tissue
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
For liquid material, refer to CoA for concentration.
Formulation
Supplied as a solution in PBS containing BSA, Glycerol and Sodium Azide *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C, as supplied.
- 1 month, 2 to 8 °C under sterile conditions after opening.
- 6 months, -20 to -70 °C under sterile conditions after opening.
Calculators
Background: BTN2A2/Butyrophilin 2A2
Long Name
Butyrophilin Subfamily 2 Member A2
Alternate Names
BTF2, BTN2, Butyrophilin 2
Gene Symbol
BTN2A2
UniProt
Additional BTN2A2/Butyrophilin 2A2 Products
Product Documents for Human BTN2A2/Butyrophilin 2A2 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human BTN2A2/Butyrophilin 2A2 Antibody
* Contains 0.1% Sodium Azide, which is not hazardous at this concentration according to GHS classifications. Refer to SDS for additional information and handling instructions.
For research use only
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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