Human BTN2A2/Butyrophilin 2A2 Antibody

Catalog # Availability Size / Price Qty
AF8645
AF8645-SP
Detection of Human BTN2A2/Butyrophilin 2A2 by Western Blot.
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Human BTN2A2/Butyrophilin 2A2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human BTN2A2/Butyrophilin 2A2 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse BTN2A2 is observed, less than 5% cross-reactivity with recombinant human (rh) BTN2A1 and rhBTN3A2 is observed, and less than 1% cross-reactivity with rhBTN1A1 is observed.
Source
Polyclonal Rabbit IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human BTN2A2/Butyrophilin 2A2
Phe34-Val237
Accession # Q8WVV5
Formulation
Supplied as a solution in PBS containing BSA, Glycerol and Sodium Azide *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1:1000 dilution
See below
Simple Western
1:25 dilution
See below
Immunohistochemistry
1:50 dilution
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human BTN2A2/Butyrophilin 2A2 by Western Blot. View Larger

Detection of Human BTN2A2/Butyrophilin 2A2 by Western Blot. Western blot shows lysates of human kidney tissue, human heart tissue, and human testis tissue. PVDF membrane was probed with a 1:1000 dilution of Rabbit Anti-Human BTN2A2/Butyrophilin 2A2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8645) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for BTN2A2/Butyrophilin 2A2 at approximately 50-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry BTN2A2/Butyrophilin 2A2 in Human Thymus. View Larger

BTN2A2/Butyrophilin 2A2 in Human Thymus. BTN2A2/Butyrophilin 2A2 was detected in immersion fixed paraffin-embedded sections of human thymus using Rabbit Anti-Human BTN2A2/Butyrophilin 2A2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8645) at a 1:50 dilution overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in endothelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western Detection  of Human BTN2A2/Butyrophilin 2A2 by Simple  WesternTM. View Larger

Detection of Human BTN2A2/Butyrophilin 2A2 by Simple WesternTM. Simple Western lane view shows lysates of human kidney tissue, human heart tissue, and human testis tissue, loaded at 0.2 mg/mL. A specific band was detected for BTN2A2/Butyrophilin 2A2 at approximately 60 kDa (as indicated) using 1:25 dilution of Rabbit Anti-Human BTN2A2/Butyrophilin 2A2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8645). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C, as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 6 months, -20 to -70 °C under sterile conditions after opening.

Background: BTN2A2/Butyrophilin 2A2

Butyrophilin subfamily 2 member A2 (BTN2A2) is a protein encoded by the BTN2A2 gene and is the major protein associated with fat droplets in the milk. This gene is a member of the BTN2 subfamily of genes, which encode proteins belonging to the butyrophilin protein family. The gene is located in a cluster on chromosome 6, consisting of seven genes belonging to the expanding B7/butyrophilin-like group, a subset of the immunoglobulin gene superfamily. The encoded protein is a type 1 receptor glycoprotein involved in lipid, fatty-acid and sterol metabolism. Several alternatively spliced transcript variants of this gene have been described.

Long Name
Butyrophilin Subfamily 2 Member A2
Entrez Gene IDs
10385 (Human); 238555 (Mouse); 306957 (Rat)
Alternate Names
BTF2; BTF2BT2.2butyrophilin 2; BTN2; BTN2A2; Butyrophilin 2; butyrophilin subfamily 2 member A2; butyrophilin, subfamily 2, member A2; FLJ41908

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Normal Rabbit IgG Control

Ctrl,CyTOF-ready

Secondary Antibodies

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