Human c-Myc Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB36961-100
MAB36961-SP
    Detection  of human c‑Myc by Western Blot.
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Human c-Myc Antibody Summary

Species Reactivity
Human
Specificity
Detects human c-Myc in direct ELISAs.
Source
Recombinant Monoclonal Rabbit IgG Clone # 2270A
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E.coli-derived recombinant human c-Myc
Arg66-Asp201
Accession # P01106
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
3-25 µg/mL
See below
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
See below
Knockout Validated
c‑Myc is specifically detected in HEK293T human embryonic kidney parental cell line but is not detectable in c‑Myc knockout HEK293T cell line.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection  of human c‑Myc by Western Blot. View Larger

Detection of human c‑Myc by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, HT‑29 human colon adenocarcinoma cell line, Jurkat human acute T cell leukemia cell line, and LNCaP human prostate cancer cell line. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human c‑Myc Monoclonal Antibody (Catalog # MAB36961) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for c‑Myc at approximately 62 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry c‑Myc  in HeLa Human Cell Line. View Larger

c‑Myc in HeLa Human Cell Line. c‑Myc was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Rabbit Anti-Human c‑Myc Monoclonal Antibody (Catalog # MAB36961) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Intracellular Staining by Flow Cytometry Detection of c‑Myc in Jurkat Human Cell Line by Flow Cytometry. View Larger

Detection of c‑Myc in Jurkat Human Cell Line by Flow Cytometry. Jurkat human acute T cell leukemia cell line was stained with Rabbit Anti-Human c‑Myc Mono­clonal Antibody (Catalog # MAB36961, filled histogram) or isotype control antibody (Catalog # MAB1050, open histogram), followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with methanol. View our protocol for Staining Intracellular Molecules.

Knockout Validated Western  Blot Shows Human c‑Myc Specificity by Using Knockout Cell Line. View Larger

Western Blot Shows Human c‑Myc Specificity by Using Knockout Cell Line. Western blot shows lysates of HEK293T human embryonic kidney parental cell line and c‑Myc knockout HEK293T cell line (KO). PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human c‑Myc Monoclonal Antibody (Catalog # MAB36961) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for c‑Myc at approximately 62 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in knockout HEK293T cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: c-Myc

Human c-Myc is a 439 amino acid transcription factor with a bHLH/LZ (basic Helix-Loop-Helix, Leucine Zipper) domain. c-Myc DNA-binding and transcription function is achieved upon heterodimerization with its partner Max. c-Myc is often over-expressed and mutated in hematopoietic tumors. Mutations frequently result in truncations that remove the transactivation region or in the bHLH/LZ domain required for association with Max and DNA. Over the region used as immunogen, human c-Myc is 92% identical to the rat and mouse c-Myc proteins.

Long Name
v-Myc Avian Myelocytomatosis Viral Oncogene Homolog (Avian)
Entrez Gene IDs
4609 (Human); 17869 (Mouse); 24577 (Rat)
Alternate Names
avian myelocytomatosis viral oncogene homolog; BHLHE39; bHLHe39MRTL; Class E basic helix-loop-helix protein 39; cMyc; c-Myc; myc proto-oncogene protein; Myc; Myc2; MYCC; myc-related translation/localization regulatory factor; Niard; Nird; Proto-oncogene c-Myc; Transcription factor p64; v-myc avian myelocytomatosis viral oncogene homolog; v-myc myelocytomatosis viral oncogene homolog (avian)

Product Datasheets

FAQs

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Cell and Tissue Staining Kits

Isotype Controls

Normal Rabbit IgG Control

Ctrl,CyTOF-ready

Reconstitution Buffers

Secondary Antibodies

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