Human c-Myc Antibody Summary
Accession # P01106
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of human c‑Myc by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, HT‑29 human colon adenocarcinoma cell line, Jurkat human acute T cell leukemia cell line, and LNCaP human prostate cancer cell line. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human c‑Myc Monoclonal Antibody (Catalog # MAB36961) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for c‑Myc at approximately 62 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
c‑Myc in HeLa Human Cell Line. c‑Myc was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Rabbit Anti-Human c‑Myc Monoclonal Antibody (Catalog # MAB36961) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of c‑Myc in Jurkat Human Cell Line by Flow Cytometry. Jurkat human acute T cell leukemia cell line was stained with Rabbit Anti-Human c‑Myc Monoclonal Antibody (Catalog # MAB36961, filled histogram) or isotype control antibody (Catalog # MAB1050, open histogram), followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with methanol. View our protocol for Staining Intracellular Molecules.
Western Blot Shows Human c‑Myc Specificity by Using Knockout Cell Line. Western blot shows lysates of HEK293T human embryonic kidney parental cell line and c‑Myc knockout HEK293T cell line (KO). PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human c‑Myc Monoclonal Antibody (Catalog # MAB36961) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for c‑Myc at approximately 62 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in knockout HEK293T cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Human c-Myc is a 439 amino acid transcription factor with a bHLH/LZ (basic Helix-Loop-Helix, Leucine Zipper) domain. c-Myc DNA-binding and transcription function is achieved upon heterodimerization with its partner Max. c-Myc is often over-expressed and mutated in hematopoietic tumors. Mutations frequently result in truncations that remove the transactivation region or in the bHLH/LZ domain required for association with Max and DNA. Over the region used as immunogen, human c-Myc is 92% identical to the rat and mouse c-Myc proteins.
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