Human c‑Myc Antibody

Detection of c‑Myc in Jurkat Human Cell Line by Flow Cytometry.

Jurkat human acute T cell leukemia cell line was stained with Rabbit Anti-Human c-Myc Mono-clonal Antibody (Catalog # MAB36961, filled histogram) or isotype control antibody (Catalog # MAB1050, open histogram), followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with methanol. View our protocol for Staining Intracellular Molecules.

Detection of Human c-Myc by Immunohistochemistry

Expression dynamics of markers which distinguish normal weight and obese patient synovial fibroblasts. (A) Expression of transcriptional regulators MYC and FOS, and secretory factors INHBA and CHI3L1 along the pseudotime axis, overlaid with representation of clusters (left) and sample distribution (right) using Monocle pseudotime trajectory. Cells are ordered in pseudotime based on differentially expressed genes (q‐value < .01). (B) FeaturePlots displaying cluster specific expression of MYC, FOS, INHBA and CHI3L1 on the t‐SNE map along with violin plots showing the expression levels (y‐axis) of these markers for each cluster (x‐axis). (C and D) Median concentrations of INHBA (inhibin) and CHI3L1 in 24‐h conditioned media from normal‐weight and obese OA SF by Luminex. Bars represent median concentration in pg/ml from n = 4 patients per cohort. (E) Representative immunofluorescence imaging for c‐Fos or c‐Myc (GOI, green) in OA hip synovial tissue from normal weight (NW) and obese (OB) patients. SF were visualized with FAP (pink), and nuclei were stained with DAPI (blue), colocalization of FAP and GOI has been pseudo‐coloured (white). See Figure S10 for additional panels. (F and G) Quantification of colocalized c‐Myc or c‐Fos with FAP labelled SF. N = 8. Means plotted ± SD and analysed by student's t‐test **p < .01. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37006170), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Human c-Myc by Immunohistochemistry

Expression dynamics of markers which distinguish normal weight and obese patient synovial fibroblasts. (A) Expression of transcriptional regulators MYC and FOS, and secretory factors INHBA and CHI3L1 along the pseudotime axis, overlaid with representation of clusters (left) and sample distribution (right) using Monocle pseudotime trajectory. Cells are ordered in pseudotime based on differentially expressed genes (q‐value < .01). (B) FeaturePlots displaying cluster specific expression of MYC, FOS, INHBA and CHI3L1 on the t‐SNE map along with violin plots showing the expression levels (y‐axis) of these markers for each cluster (x‐axis). (C and D) Median concentrations of INHBA (inhibin) and CHI3L1 in 24‐h conditioned media from normal‐weight and obese OA SF by Luminex. Bars represent median concentration in pg/ml from n = 4 patients per cohort. (E) Representative immunofluorescence imaging for c‐Fos or c‐Myc (GOI, green) in OA hip synovial tissue from normal weight (NW) and obese (OB) patients. SF were visualized with FAP (pink), and nuclei were stained with DAPI (blue), colocalization of FAP and GOI has been pseudo‐coloured (white). See Figure S10 for additional panels. (F and G) Quantification of colocalized c‐Myc or c‐Fos with FAP labelled SF. N = 8. Means plotted ± SD and analysed by student's t‐test **p < .01. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37006170), licensed under a CC-BY license. Not internally tested by R&D Systems.