Human C-Peptide Antibody

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C-Peptide in Human Pancreas.
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Product Details
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Human C-Peptide Antibody Summary

Species Reactivity
Detects human C-Peptide in direct ELISAs.
Monoclonal Mouse IgG1 Clone # 915612
Protein A or G purified from hybridoma culture supernatant
Human C-Peptide synthetic peptide
Accession # P01308
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry C-Peptide antibody in Human Pancreas by Immunohistochemistry (IHC-P). View Larger

C-Peptide in Human Pancreas. C-Peptide was detected in immersion fixed paraffin-embedded sections of human pancreas using Mouse Anti-Human C-Peptide Monoclonal Antibody (Catalog # MAB80561) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to islets. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: C-Peptide

Insulin is a peptide hormone that facilitates the cellular uptake of glucose by regulating the appearance of membrane glucose transporters. The single chain insulin propeptide consists of a 30 amino acid B chain (aa 25-54), a C-peptide (aa 55-89), and a 21 aa A chain (aa 90-110). Removal of the C-peptide by proteolysis enables the formation of mature Insulin, a disulfide-linked heterodimer of the A and B chains. Circulating C-peptide levels are elevated in hyperinsulinism, obesity, and type II diabetes. The human Insulin C-peptide shares 61% and 68% aa sequence identity with mouse and rat Insulin C-peptide, respectively.

Long Name
Proinsulin C-Peptide
Entrez Gene IDs
3630 (Human); 16333 (Mouse); 397415 (Porcine); 280829 (Bovine); 483665 (Canine)
Alternate Names
C-P; C-Peptide; IDDM2; ILPR; Insulin C-Peptide; insulin; IRDN; MODY10; Proinsulin C-Peptide; proinsulin

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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