|C-Peptide in Human Pancreas. C-Peptide was detected in immersion fixed paraffin-embedded sections of human pancreas using Mouse Anti-Human C-Peptide Monoclonal Antibody (Catalog # MAB80561) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to islets. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.|
Insulin is a peptide hormone that facilitates the cellular uptake of glucose by regulating the appearance of membrane glucose transporters. The single chain insulin propeptide consists of a 30 amino acid B chain (aa 25-54), a C-peptide (aa 55-89), and a 21 aa A chain (aa 90-110). Removal of the C-peptide by proteolysis enables the formation of mature Insulin, a disulfide-linked heterodimer of the A and B chains. Circulating C-peptide levels are elevated in hyperinsulinism, obesity, and type II diabetes. The human Insulin C-peptide shares 61% and 68% aa sequence identity with mouse and rat Insulin C-peptide, respectively.
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