Human Cadherin-6/KCAD Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human Cadherin‑6/KCAD in direct ELISAs.
  • Source
    Monoclonal Mouse IgG3 Clone # 427903
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human Cadherin‑6/KCAD
    Ser54-Ala615
    Accession # P55285
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in Tris and NaCl with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Immunohistochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
Cadherin‑6/KCAD in Human Kidney Cancer Tissue. Cadherin‑6/KCAD was detected in immersion fixed paraffin-embedded sections of human kidney cancer tissue using 25 µg/mL Mouse Anti-Human Cadherin‑6/KCAD Monoclonal Antibody (Catalog # MAB27151) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Immunohistochemistry
Cadherin‑6/KCAD in Human Liver Cancer Tissue. Cadherin‑6/KCAD was detected in immersion fixed paraffin-embedded sections of human liver cancer tissue using 8 µg/mL Mouse Anti-Human Cadherin‑6/KCAD Monoclonal Antibody (Catalog # MAB27151) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Cadherin-6/KCAD

The cadherin superfamily is a large family of membrane-associated glycoproteins that engage in homotypic, calcium‑dependent, cell-to-cell adhesion events. The superfamily can be divided into at least four subfamilies based on the extracellular (EC) regions and cytoplasmic domains (1, 2). These include classical cadherins, desmosomal cadherins, protocadherins, and cadherin-like molecules that contain a variable number of EC and transmembrane (TM) domains (1). Cadherin‑6, also known as KCAD or K-cadherin, is a classical cadherin of 110‑120 kD that has at least one full length and two alternate splice forms ranging in size from 105‑120 kDa (3). Human Cadherin-6 is synthesized as a 790 amino acid (aa) type I transmembrane glycoprotein that contains a 18 aa signal peptide, a 35 aa prosequence, a 562 aa extracellular region, a 21 aa transmembrane segment, and a 154 aa cytoplasmic domain (4, 5). There are five EC cadherin domains that are approximately 110 aa in length. This pattern is consistent with classical cadherin family molecules that are modular in their extracellular region and mediate calcium‑dependent cell‑to‑cell adhesion through their Ca++-binding repeats (2). Due to the absence of a His-Ala-Val motif in its most N-terminal cadherin repeat, Cadherin-6 can be further classified as a type II classical cadherin (4). One Cadherin‑6 splice variant (termed 6/2) shows a 9 aa substitution for the 94 aa that span residues 283 to 376 of the full-length extracellular region (3). A second splice variant shows a 36 aa substitution for the C-terminal 163 aa of the transmembrane and cytoplasmic region (6). Human Cadherin‑6 shares 98% aa sequence identity with rat Cadherin‑6, plus 60% and 58% aa identity with human cadherin 8 and 11, respectively, within the extracellular regions. Cadherin-6 has high expression in kidney, brain, and cerebellum, and low expression in lung, pancreas, gastric mucosa, and cytotrophoblasts (4, 5, 7, 8, 9). Cadherin-6 is also found in renal, lung, and ovariancarcinoma (7, 10). As a classic cadherin, Cadherin-6 will form homodimers and promote intercellular adhesion with itself and possibly cadherin-9 and -14 (4, 11).

  • References:
    1. Koch, A.W. et al. (2004) Cell. Mol. Life Sci. 61:1884.
    2. Angst, B.D. et al. (2001) J. Cell Sci. 114:629.
    3. Mbalaviele, G. et al. (1998) J. Cell Biol. 141:1467.
    4. Shimoyama, Y. et al. (2000) Biochem. J. 349:159.
    5. Shimoyama, Y. et al. (1995) Cancer Res. 55:2206.
    6. GenBank Accession # P55285.
    7. Xiang Y.Y. et al. (1994) Cancer Res. 54:3034.
    8. Marthiens V. et al. (2002) Mol. Cell Neurosci. 20:458.
    9. MacCalman C.D. et al. (1998) Am J Reprod. Immunol. 39:96.
    10. Sella, G.C. et al. (2001) Cancer Res. 61:6977.
    11. Shimoyama, Y. et al. (1999) J. Biol. Chem. 274:11987.
  • Entrez Gene IDs:
    1004 (Human)
  • Alternate Names:
    cadherin 6, type 2, K-cadherin (fetal kidney); cadherin, fetal kidney; Cadherin6; Cadherin-6; CDH6; KCAD; K-cadherin (fetal kidney); K-Cadherin
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