Human Calcitonin Antibody Summary
Accession # P01258
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Calcitonin in Human Dorsal Root Ganglion. Calcitonin was detected in immersion fixed paraffin-embedded sections of human dorsal root ganglion using Rabbit Anti-Human Calcitonin Monoclonal Antibody (Catalog # MAB83503) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Human Calcitonin (CALCA, Calcitonin) is a 32 aminoacids (aa) protein formed by the proteolytic cleavage of a larger prepropeptide, which is the product of the CALC1 gene. Precursor protein (a 141 aa in length member of the calcitonin family) is cleaved into a propeptide and the following 2 chains: Calcitonin (aa 85-116) and Katacalcin (aa 121-141). Antigen used to develop this antibody corresponds to the mature Calcitonin cleaved peptide. Secreted Calcitonin is known to cause a rapid but short-lived drop in the level of calcium and phosphate in blood by promoting the incorporation of those ions in the bones.
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