Human Calcitonin Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB83503-100
MAB83503-SP
Calcitonin  in Human Dorsal Root Ganglion.
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Human Calcitonin Antibody Summary

Species Reactivity
Human
Specificity
Detects human Calcitonin in direct ELISAs.
Source
Recombinant Monoclonal Rabbit IgG Clone # 2413G
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Human Calcitonin synthetic peptide
Accession # P01258
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunohistochemistry
3-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunohistochemistry Calcitonin  in Human Dorsal Root Ganglion. View Larger

Calcitonin in Human Dorsal Root Ganglion. Calcitonin was detected in immersion fixed paraffin-embedded sections of human dorsal root ganglion using Rabbit Anti-Human Calcitonin Monoclonal Antibody (Catalog # MAB83503) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Calcitonin

Human Calcitonin (CALCA, Calcitonin) is a 32 aminoacids (aa) protein formed by the proteolytic cleavage of a larger prepropeptide, which is the product of the CALC1 gene. Precursor protein (a 141 aa in length member of the calcitonin family) is cleaved into a propeptide and the following 2 chains: Calcitonin (aa 85-116) and Katacalcin (aa 121-141). Antigen used to develop this antibody corresponds to the mature Calcitonin cleaved peptide. Secreted Calcitonin is known to cause a rapid but short-lived drop in the level of calcium and phosphate in blood by promoting the incorporation of those ions in the bones.

Long Name
Calcitonin Related Polypeptide Alpha
Entrez Gene IDs
796 (Human); 12310 (Mouse); 24241 (Rat)
Alternate Names
Alpha-type CGRP; ATP2B2plasma membrane calcium ATPase; ATPase, Ca++ transporting, plasma membrane 4; CALC1; CALCA; Calcitonin; CGRP; CGRP-I; DKFZp686G08106; DKFZp686M088; EC 3.6.3; EC 3.6.3.8; Katacalcin; Matrix-remodeling-associated protein 1; matrix-remodelling associated 1; MXRA1; PCT; Plasma membrane calcium ATPase isoform 4; Plasma membrane calcium pump isoform 4; plasma membrane calcium pump; plasma membrane calcium-transporting ATPase 4; PMCA4b; PMCA4PMCA4x; sarcolemmal calcium pump

Product Datasheets

FAQs

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Normal Rabbit IgG Control

Ctrl,CyTOF-ready

Reconstitution Buffers

Secondary Antibodies

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