|Detection of Human Calcium Activated Nucleotidase 1/CANT1 by Western Blot. Western blot shows lysates of LNCaP human prostate cancer cell line and U2OS human osteosarcoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Calcium Activated Nucleotidase 1/CANT1 Monoclonal Antibody (Catalog # MAB6720) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for Calcium Activated Nucleotidase 1/CANT1 at approximately 40-45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.|
|Calcium Activated Nucleotidase 1/CANT1 in PC‑3 Human Cell Line. Calcium Activated Nucleotidase 1/CANT1 was detected in immersion fixed PC‑3 human prostate cancer cell line using Mouse Anti-Human Calcium Activated Nucleotidase 1/CANT1 Monoclonal Antibody (Catalog # MAB6720) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to the perinuclear region. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.|
|Detection of Human Calcium Activated Nucleotidase 1/CANT1 by Simple WesternTM. Simple Western lane view shows lysates of LNCaP human prostate cancer cell line, loaded at 0.5 mg/mL. A specific band was detected for Calcium Activated Nucleotidase 1/CANT1 at approximately 52 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human Calcium Activated Nucleotidase 1/CANT1 Monoclonal Antibody (Catalog # MAB6720). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.|
CANT1 (soluble Calcium-Activated NucleoTidase 1; also SCAN-1, Apyrase homolog and NF kappa B-activating protein 107) is a 45-48 kDa glycoprotein member of the apyrase (ADP/ATP hydrolysing) family of molecules. It is an ER and Golgi-embedded protein that hydrolyzes UDP in a calcium-dependent manner, and appears to be involved in the regulation of protein glycosylation and folding. It is also reported that CANT1 is essential for cell proliferation. CANT1 is not ubiquitous, but is found in multiple cell types, including prostatic epithelium, fibroblasts, lymphocytes and chondrocytes. Human CANT1 is a 401 amino acid (aa) type II transmembrane glycoprotein. It contains a 44 aa N-terminal cytoplasmic region that possesses an ER retention motif (aa 38-42), and a 339 aa luminal domain (aa 63-401). CANT1 is reported to form membrane-bound disulfide-linked homodimers, and potentially forms non-covalent homodimers in a soluble (circulating) state. The soluble form is 35‑40 kDa in size, and presumably cleaved near the transmembrane segment. However, multiple splice forms are possible, and may account for the lower MWs. Two splice forms impact the extracellular region, one which contains a four aa substitution for aa 219-401, and a second that possesses a 26 aa substitution for aa 220‑401. Two additional splice forms utilize alternative start sites at Met 31 and Met165. Over aa 80-401, human CANT1 shares 89% aa sequence identity with mouse CANT1.