Human Calcium-sensing R/CaSR Antibody Summary
Accession # P41180-1
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Calcium-sensing R/CaSR in HEK293 Human Cell Line Transfected with Human Calcium-sensing R/CaSR and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) human Calcium-sensing R/CaSR or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human Calcium-sensing R/CaSR Monoclonal Antibody (Catalog # MAB9608) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB003). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Calcium-sensing R/CaSR
Calcium-sensing receptor (CasR) is a plasma membrane G-protein-coupled receptor that senses changes in the extracellular concentration of calcium ions and plays a key role in maintaining calcium homeostasis (1). CasR is expressed in the parathyroid hormone-producing chief cells of the parathyroid gland, and the cells lining the kidney tubule (2). Mutations in this gene cause familial hypocalciuric hypercalcemia, familial, isolated hypoparathyroidism, and neonatal severe primary hyperparathyroidism (3). The full length of CasR has a large extracellular N-terminal domain, a central region of 7 transmembrane domains, and a long intracellular C‑terminal domain. The amino acid 20-601 contains the extracellular domain with multiple glycosylation sites (4). Human CasR shares 96.1% and 96.5% aa sequence identity with mouse and rat CasR, respectively.
- Geng, Y. et al. (2016) Elife 5:e13662.
- Hendy, G. N. et al. (2000) Hum. Mutat. 16:281.
- Brown, E. M. et al. (1993) Nature 366:575.
- Garrett, J. E.et al. (1995) J. Biol. Chem. 270:12919.
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