Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 611825
View all Calcium-sensing R/CaSR Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived transfected with recombinant human Calcium-sensing R/CaSR protein
Tyr20-Lys601
Accession # P41180-1

Specificity

Detects human Calcium-sensing R/CaSR protein in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human Calcium-sensing R/CaSR Antibody

Detection of Calcium-sensing R/CaSR antibody in HEK293 Human Cell Line Transfected with Human Calcium-sensing R/CaSR and eGFP antibody by Flow Cytometry.

Detection of Calcium-sensing R/CaSR in HEK293 Human Cell Line Transfected with Human Calcium-sensing R/CaSR and eGFP by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with either (A) human Calcium-sensing R/CaSR or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human Calcium-sensing R/CaSR Monoclonal Antibody (Catalog # MAB9608) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB003). View our protocol for Staining Membrane-associated Proteins.

Applications for Human Calcium-sensing R/CaSR Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: HEK293 Human Cell Line Transfected with Human Calcium-sensing R/CaSR and eGFP

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Calcium-sensing R/CaSR

Calcium-sensing receptor (CasR) is a plasma membrane G-protein-coupled receptor that senses changes in the extracellular concentration of calcium ions and plays a key role in maintaining calcium homeostasis (1). CasR is expressed in the parathyroid hormone-producing chief cells of the parathyroid gland, and the cells lining the kidney tubule (2). Mutations in this gene cause familial hypocalciuric hypercalcemia, familial, isolated hypoparathyroidism, and neonatal severe primary hyperparathyroidism (3). The full length of CasR has a large extracellular N-terminal domain, a central region of 7 transmembrane domains, and a long intracellular C‑terminal domain. The amino acid 20-601 contains the extracellular domain with multiple glycosylation sites (4). Human CasR shares 96.1% and 96.5% aa sequence identity with mouse and rat CasR, respectively.

References

  1. Geng, Y. et al. (2016) Elife 5:e13662.
  2. Hendy, G. N. et al. (2000) Hum. Mutat. 16:281.
  3. Brown, E. M. et al. (1993) Nature 366:575.
  4. Garrett, J. E.et al. (1995) J. Biol. Chem. 270:12919.

Long Name

Calcium-sensing Receptor

Alternate Names

CAR, CaSR, EIG8, FHH, FIH, GPRC2A, HHC1, NSHPT, PCaR1

Entrez Gene IDs

846 (Human); 12374 (Mouse); 24247 (Rat)

Gene Symbol

CASR

UniProt

P41180-1

Additional Calcium-sensing R/CaSR Products

Product Documents for Human Calcium-sensing R/CaSR Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Calcium-sensing R/CaSR Antibody

For research use only

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