Human Calcium-sensing R/CaSR Antibody

Catalog # Availability Size / Price Qty
MAB9608-100
MAB9608-SP
Human Calcium-sensing R/CaSR Antibody in Flow Cytometry
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Human Calcium-sensing R/CaSR Antibody Summary

Species Reactivity
Human
Specificity
Detects human Calcium-sensing R/CaSR protein in direct ELISAs.
Source
Monoclonal Mouse IgG2A Clone # 611825
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived transfected with recombinant human Calcium-sensing R/CaSR protein
Tyr20-Lys601
Accession # P41180-1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of Calcium-sensing R/CaSR  in HEK293 Human Cell Line Transfected with Human Calcium-sensing R/CaSR and eGFP by Flow  Cytometry. View Larger

Detection of Calcium-sensing R/CaSR in HEK293 Human Cell Line Transfected with Human Calcium-sensing R/CaSR and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) human Calcium-sensing R/CaSR or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human Calcium-sensing R/CaSR Monoclonal Antibody (Catalog # MAB9608) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB003). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Calcium-sensing R/CaSR

Calcium-sensing receptor (CasR) is a plasma membrane G-protein-coupled receptor that senses changes in the extracellular concentration of calcium ions and plays a key role in maintaining calcium homeostasis (1). CasR is expressed in the parathyroid hormone-producing chief cells of the parathyroid gland, and the cells lining the kidney tubule (2). Mutations in this gene cause familial hypocalciuric hypercalcemia, familial, isolated hypoparathyroidism, and neonatal severe primary hyperparathyroidism (3). The full length of CasR has a large extracellular N-terminal domain, a central region of 7 transmembrane domains, and a long intracellular C‑terminal domain. The amino acid 20-601 contains the extracellular domain with multiple glycosylation sites (4). Human CasR shares 96.1% and 96.5% aa sequence identity with mouse and rat CasR, respectively.

References
  1. Geng, Y. et al. (2016) Elife 5:e13662.
  2. Hendy, G. N. et al. (2000) Hum. Mutat. 16:281.
  3. Brown, E. M. et al. (1993) Nature 366:575.
  4. Garrett, J. E.et al. (1995) J. Biol. Chem. 270:12919.
Long Name
Calcium-sensing Receptor
Entrez Gene IDs
846 (Human); 12374 (Mouse); 24247 (Rat)
Alternate Names
calcium-sensing receptor; CAR; CaSR; EIG8; extracellular calcium-sensing receptor; FHH; FIH; GPRC2A; GPRC2AHHC; HHC1; hypocalciuric hypercalcemia 1; NSHPT; parathyroid Ca(2+)-sensing receptor 1; Parathyroid cell calcium-sensing receptor; PCaR1; PCAR1MGC138441

Product Datasheets

FAQs

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Isotype Controls

Mouse IgG2A Isotype Control

Reconstitution Buffers

Secondary Antibodies

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