Key Product Details

Validated by

Orthogonal Validation, Biological Validation

Species Reactivity

Human

Applications

Dual RNAscope ISH-IHC Compatible, Immunocytochemistry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1112I
View all Caspase-3 Primary Antibodies »

Product Specifications

Immunogen

KLH-conjugated human Caspase-3 synthetic peptide
CRGTELDCGIETD
Accession # P42574

Specificity

Detects the active form and p17 subunit of Caspase-3 in direct ELISAs, with less than 5% cross-reactivity with the precursor form observed.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human Caspase‑3 Antibody

Caspase-3 antibody in Jurkat Human Cell Line by Immunocytochemistry (ICC).

Caspase‑3 in Jurkat Human Cell Line.

Caspase-3 was detected in immersion fixed Jurkat human acute T cell leukemia cell line treated with Staurosporine using Rabbit Anti-Human Caspase-3 Monoclonal Antibody (Catalog # MAB7071) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Caspase-3 antibody in Human Colon Using Dual RNAscope®ISH and IHC.

Caspase-3 in Human Colon Using Dual RNAscope®ISH and IHC.

Caspase-3 mRNA was detected (top image) in formalin-fixed paraffin-embedded tissue sections of human colon probed with ACD RNAScope®Probe (Catalog # 436561) and stained using ACD RNAscope®2.5 HD Duplex Detection Reagents (red, Catalog # 322500). Adjacent tissue section (bottom image) was processed for immunohistochemistry using R&D Systems Rabbit Anti-Human Caspase-3 Monoclonal Antibody (Catalog# MAB7071) at 5 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte HRP Polymer Antibody (R&D Systems, Catalog # VC001) and DAB chromogen (brown). Tissues were counterstained with hematoxylin (blue).

Applications for Human Caspase‑3 Antibody

Application
Recommended Usage

Dual RNAscope ISH-IHC Compatible

5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human colon

Immunocytochemistry

1-25 µg/mL
Sample: Immersion fixed Jurkat human acute T cell leukemia cell line treated with Staurosporine

Reviewed Applications

Read 1 review rated 5 using MAB7071 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: Caspase-3

Caspase-3 (Cysteine-aspartic acid protease 3/Casp3; also Yama, apopain and CPP32) is a 29 kDa heterodimer that belongs to the peptidase C14A family of enzymes. It is widely expressed and considered to be the major executioner caspase in the apoptotic cascade. Human procaspase-3 is a 32 kDa, 277 amino acid (aa) protein and is normally an inactive homodimer. Following cell stress/activation, procaspase-3 undergoes proteolysis to generate an N-terminal 148 aa p17/17 kDa subunit (aa 29-175), plus a 102 aa C-terminal p12/12 kDa subunit. These subunits noncovalently heterodimerize, and associate with another p17/p12 heterodimer to form an active enzyme. There is one potential variant that shows an alternative start site nine aa upstream of the standard start site coupled with a 21 aa substitution for aa 162-277. Over aa 29-175, human and mouse caspase-3 share 87% aa identity.

Alternate Names

Apopain, CASP3, Caspase3, CPP32, LICE-1, YAMA

Entrez Gene IDs

836 (Human); 12367 (Mouse)

Gene Symbol

CASP3

UniProt

P42574 (Rabbit)

Additional Caspase-3 Products

Product Documents for Human Caspase‑3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Caspase‑3 Antibody

For research use only

Related Research Areas

Cysteine Proteases and Regulators

Customer Reviews for Human Caspase‑3 Antibody (1)

5 out of 5
1 Customer Rating
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used Human Caspase‑3 Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 1 of 1 review Showing All
Filter By:
Clear Filters X
  • Human Caspase-3 Antibody
    Name: Preethi Vijayaraj
    Application: Immunohistochemistry
    Sample Tested: Adult lung
    Species: Human
    Verified Customer | Posted 11/04/2016
    Human Caspase‑3 Antibody MAB7071

There are no reviews that match your criteria.

Showing  1 - 1 of 1 review Showing All

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies