Detects human CCL15/MIP‑1δ in direct ELISAs and Western blots. In direct ELISAs and Western blots, a preference for the 68 aa residue isoform over the 92 aa residue isoform of human CCL15/LKN-1 is shown. Additionally, in direct ELISAs, no cross-reactivity with ____________ is observed. Neutralizes the biological activity of the 68 aa isoform of recombinant human (rh) CCL15/LKN-1. It will also neutralize the biological activity of the 92 aa residue isoform of rhCCL15/LKN-1 using a 2-fold higher Ig concentration.
Polyclonal Goat IgG
E. coli-derived recombinant human CCL15/MIP-1δ Ser46-Ile113 Accession # Q16663
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
<0.10 EU per 1 μg of the antibody by the LAL method.
Recombinant Human CCL15/MIP‑1δ 68 aa (Catalog # 628-LK)
Measured by its ability to neutralize CCL15/MIP‑1δ-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR1. The Neutralization Dose (ND50) is typically 0.5-2.5 µg/mL in the presence of 15 ng/mL Recombinant Human CCL15/MIP‑1δ 68 aa.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Chemotaxis Induced by CCL15/MIP‑1δ and Neutralization by Human CCL15/MIP‑1δ Antibody. Recombinant Human CCL15/MIP‑1δ (Catalog # 628‑LK) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL15/MIP‑1δ (15 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CCL15/MIP‑1δ Antigen Affinity-purified Polyclonal Antibody (Catalog # AF628). The ND50 is typically 0.5-2.5 µg/mL.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL15/MIP-1 delta
Macrophage inflammatory protein 1 delta (MIP-1 delta), also named Leukotactin-1 (LKN-1), MIP-5, HCC-2, and NCC-3, together with mouse C10, mouse MIP-1 gamma and human MPIF-1, constitute a subgroup of CC chemokines which contain six instead of four conserved cysteine residues. The two additional cysteine residues in MIP-1 delta/LKN-1 form a third disulfide bond.
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.