Human CD14 Antibody

Name: Human CD14 Antibody
Brand: R&D Systems
SKU: MAB3833R
Price: 369 USD
Availability: InStock

R&D Systems | Catalog # MAB3833R

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Product Documents

Recombinant Monoclonal Antibody.

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Product Specifications
Applications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human

Applications

ELISA Capture (Matched Antibody Pair)

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG₁ Clone # 50040R

View all CD14 Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human CD14
Thr20-Cys352
Accession # P08571

Specificity

Detects human CD14 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Applications for Human CD14 Antibody

Human CD14 Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)

Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:

Detection Reagent: Human CD14 Biotinylated Antibody (Catalog # BAF383)
Standard: Recombinant Human CD14 Protein (Catalog # 383-CD)

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: CD14

CD14 occurs both as a secreted protein and as a GPI-linked membrane protein. It plays a critical role in lipopolysaccharide (LPS)-dependent signal transduction.