Human CD200 Antibody

Catalog # Availability Size / Price Qty
MAB27243-SP
MAB27243-100
MAB27243-500
Product Details
Citations (1)
FAQs
Supplemental Products
Reviews

Human CD200 Antibody Summary

Species Reactivity
Human
Specificity
Detects human CD200 in ELISAs. In sandwich immunoassays, no cross-reactivity or interference with recombinant mouse CD200 is observed.
Source
Monoclonal Mouse IgG1 Clone # 325531
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human CD200
Gln31-Gly232
Accession # P41217.3
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.01 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Blockade of Receptor-ligand Interaction
In a functional ELISA, 0.2-1 µg/mL of this antibody will block 50% of the binding of 2.5 ng/mL of Recombinant Human CD200 (Catalog # 2724-CD) to immobilized Recombinant Human CD200 R (Catalog # 3414-CD) coated at 2 µg/mL (100 µL/well). At 30 μg/mL, this antibody will block >90% of the binding.
 

Human CD200 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Human CD200 Biotinylated Antibody (Catalog # BAM27242)

Standard: Recombinant Human CD200 Fc Chimera Protein, CF (Catalog # 2724-CD)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD200

CD200, also known as OX-2, is a 45 kDa transmembrane immunoregulatory protein that belongs to the immunoglobulin superfamily (1, 2). The human CD200 cDNA encodes a 278 amino acid (aa) precursor that includes a 30 aa signal sequence, a 202 aa extracellular domain (ECD), a 27 aa transmembrane segment, and a 19 aa cytoplasmic domain. The ECD is composed of one Ig-like V-type domain and one Ig-like C2-type domain (3). A splice variant of CD200 has been described and has a truncated cytoplasmic tail. Within the ECD, human CD200 shares 76% aa sequence identity with mouse and rat CD200. CD200 is widely but not ubiquitously expressed (4). Its receptor (CD200R) is restricted primarily to mast cells, basophils, macrophages, and dendritic cells, which suggests myeloid cell regulation as the major function of CD200 (5‑7). CD200 knockout mice are characterized by increased macrophage number and activation and are predisposed to autoimmune disorders (8). CD200 and CD200R associate via their respective N-terminal Ig-like domains (9). In myeloid cells, CD200R initiates inhibitory signals following receptor‑ligand contact (6, 7, 10). In T cells, however, CD200 functions as a costimulatory molecule independent of the CD28 pathway (11). Several additional CD200R-like molecules have been identified in human and mouse, but their capacity to interact with CD200 is controversial (12, 13). Several viruses encode CD200 homologs which are expressed on infected cells during the lytic phase (14, 15). Like CD200 itself, viral CD200 homologs also suppress myeloid cell activity, enabling increased viral propagation (5, 14‑16).

References
  1. Gorczynski, R.M. (2005) Curr. Opin. Invest. Drugs 6:483.
  2. Barclay, A.N. et al. (2002) Trends Immunol. 23:285.
  3. McCaughan, G.W. et al. (1987) Immunogenetics 25:329.
  4. Wright, G.J. et al. (2001) Immunology 102:173.
  5. Shiratori, I. et al. (2005) J. Immunol. 175:4441.
  6. Cherwinski, H.M. et al. (2005) J. Immunol. 174:1348.
  7. Fallarino, F. et al. (2004) J. Immunol. 173:3748.
  8. Hoek, R.M. et al. (2000) Science 290:1768.
  9. Hatherley, D. and A.N. Barclay (2004) Eur. J. Immunol. 34:1688.
  10. Jenmalm, M.C. et al. (2006) J. Immunol. 176:191.
  11. Borriello, F. et al. (1997) J. Immunol. 158:4548.
  12. Gorczynski, R. et al. (2004) J. Immunol. 172:7744.
  13. Hatherley, D. et al. (2005) J. Immunol. 175:2469.
  14. Foster-Cuevas, M. et al. (2004) J. Virol. 78:7667.
  15. Cameron, C.M. et al. (2005) J. Virol. 79:6052.
  16. Langlais, C.L. et al. (2006) J. Virol. 80:3098.
Entrez Gene IDs
4345 (Human); 17470 (Mouse); 102146004 (Cynomolgus Monkey)
Alternate Names
antigen identified by monoclonal MRC OX-2; CD200 antigenMOX1; CD200 molecule; CD200; MOX1; MOX2; MOX2MRC; MRC OX-2 antigen; MRC; OX-2 membrane glycoprotein; OX-2

Product Datasheets

Citation for Human CD200 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Multiple inhibitory ligands induce impaired T-cell immunologic synapse function in chronic lymphocytic leukemia that can be blocked with lenalidomide: establishing a reversible immune evasion mechanism in human cancer.
    Authors: Ramsay, Alan G, Clear, Andrew J, Fatah, Rewas, Gribben, John G
    Blood, 2012;120(7):1412-21.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human CD200 Antibody

There are currently no reviews for this product. Be the first to review Human CD200 Antibody and earn rewards!

Have you used Human CD200 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review