Human CD24 Antibody

Catalog # Availability Size / Price Qty
MAB5247-100
MAB5247-SP
Detection of CD24 in Human Blood Lymphocytes by Flow Cytometry.
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Human CD24 Antibody Summary

Species Reactivity
Human
Specificity
Detects human CD24
Source
Monoclonal Mouse IgG2A Clone # ML5
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human CD24
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of CD24 antibody in Human Blood Lymphocytes antibody by Flow Cytometry. View Larger

Detection of CD24 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD19 PE-conjugated Monoclonal Antibody (Catalog # FAB4867P) and either (A) Mouse Anti-Human CD24 Monoclonal Antibody (Catalog # MAB5247) or (B) Mouse IgG2A Isotype Control Antibody (Catalog # MAB003) followed by APC-conjugated Goat anti-Mouse IgG secondary antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD24

CD24, also known as heat stable antigen (HSA), is a heavily and variably glycosylated 35‑60 kDa GPI-linked sialoprotein that is expressed on B cells, T cells, keratinocytes, and myofiber synaptic nuclei and is up‑regulated in a wide variety of cancers. It is also a marker for exosomes released into the urine and amniotic fluid. CD24 binds to P-Selectin on activated platelets and vascular endothelial cells. CD24 ligation induces tumor cell apoptosis and is important for the deletion of autoreactive T cells. Mature human CD24 shares 30% and 42% aa sequence identity with mouse and rat CD24, respectively.

Entrez Gene IDs
100133941 (Human); 12484 (Mouse)
Alternate Names
CD 24; CD24 antigen (small cell lung carcinoma cluster 4 antigen); CD24 antigen; CD24 molecule; CD24; CD24A; CD24Asignal transducer CD24; FLJ22950; FLJ43543; MGC75043; Small cell lung carcinoma cluster 4 antigen

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

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Isotype Controls

Mouse IgG2A Isotype Control

Reconstitution Buffers

Secondary Antibodies

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