Human CD30/TNFRSF8 Antibody MAB229: R&D Systems

Human CD30/TNFRSF8 Antibody

(1 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human CD30/TNFRSF8 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse CD30, recombinant human (rh) CD27, and rhCD40 is observed.
  • Source
    Monoclonal Mouse IgG2B Clone # 81337
  • Purification
    Protein A or G purified from ascites
  • Immunogen
    S. frugiperda insect ovarian cell line Sf 21-derived recombinant human CD30/TNFRSF8
    Phe19-Lys379
    Accession # P28908
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.15 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Human CD30/TNFRSF8 Fc Chimera (Catalog # 813-CD)
    under non-reducing conditions only
  • Flow Cytometry
    0.25 µg/106 cells
    See below
  • Agonist Activity
    Measured by its ability to stimulate human IL-6 secretion by HDLM-2 human Hodgkin's lymphoma cells. A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272. The ED50 for this effect is typically
    0.05 - 0.2 μg/mL.
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of CD30/TNFRSF8 in Jurkat Human Cell Line by Flow Cytometry. Jurkat human acute T cell leukemia cell line was stained with Mouse Anti-Human CD30/TNFRSF8 Monoclonal Antibody (Catalog # MAB229, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.
Human CD30/TNFRSF8 AntibodyEnhances IL-6 Secretion in HDLM-2 Cells. Human CD30/TNFRSF8Monoclonal Antibody enhances IL-6 secretion in the HDLM-2 human Hodgkin'slymphoma cell line, in a dose-dependent manner, as measured using theQuantikine Human IL-6 ELISA Kit (Catalog #D6050).The ED50for this effect is typically 0.05-
0.2 μg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD30/TNFRSF8

CD30, also known as Ki-1 antigen and TNFRSF8, is a 120 kDa type I transmembrane glycoprotein belonging to the TNF receptor superfamily (1, 2). Mature human CD30 consists of a 361 amino acid (aa) extracellular domain (ECD) with six cysteine-rich repeats, a 28 aa transmembrane segment, and a 188 aa cytoplasmic domain (3). In contrast, mouse and rat CD30 lack 90 aa of the ECD and contain only three cysteine-rich repeats. Within common regions of the ECD, human CD30 shares 53% and 49% aa sequence identity with mouse and rat CD30, respectively. Alternate splicing of human CD30 generates an isoform that includes only the C‑terminal 132 aa of the cytoplasmic domain. CD30 is normally expressed on antigen-stimulated Th cells and B cells (4 - 6). However, it is upregulated in Hodgkin’s disease (on Reed-Sternberg cells), other lymphomas, chronic inflammation, and autoimmunity (7). CD30 binds to CD30 Ligand/TNFSF8 which is expressed on activated Th cells, monocytes, granulocytes and medullary thymic epithelial cells (1, 5). CD30 signaling costimulates antigen-induced Th0 and Th2 proliferation and cytokine secretion but favors a Th2-biased immune response (8). In the absence of antigenic stimulation, it can still induce T cell expression of IL-13 (9). CD30 contributes to thymic negative selection by inducing the apoptotic cell death of CD4+CD8+ T cells (10, 11). In B cells, CD30 ligation promotes cellular proliferation and antibody production in addition to the expression of CXCR4, CCL3, and CCL5 (5, 12). An 85-90 kDa soluble form of CD30 is shed from the cell surface by TACE-mediated cleavage (13, 14). Soluble CD30 retains the ability to bind CD30 Ligand and functions as an inhibitor of normal CD30 signaling (15).

  • References:
    1. Kennedy, M.K. et al. (2006) Immunology 118:143.
    2. Tarkowski, M. (2003) Curr. Opin. Hematol. 10:267.
    3. Durkop, H. et al. (1992) Cell 68:421.
    4. Hamann, D. et al. (1996) J. Immunol. 156:1387.
    5. Shanebeck, S.D. et al. (1995) Eur. J. Immunol. 25:2147.
    6. Gruss, H.-J. et al. (1994) Blood 83:2045.
    7. Oflzoglu E. et al. (2009) Adv. Exp. Med. Biol. 647:174.
    8. Del Prete, G. et al. (1995) J. Exp. Med. 182:1655.
    9. Harlin, H. et al. (2002) J. Immunol. 169:2451.
    10. Amakawa, R. et al. (1996) Cell 84:551.
    11. Chiarle, R. et al. (1999) J. Immunol. 163:194.
    12. Vinante, F. et al. (2002) Blood 99:52.
    13. Hansen, H.P. et al. (1995) Int. J. Cancer 63:750.
    14. Hansen, H.P. et al. (2000) J. Immunol. 165:6703.
    15. Hargreaves, P.G. and A. Al-Shamkhani (2002) Eur. J. Immunol. 32:163.
  • Entrez Gene IDs:
    943 (Human); 21941 (Mouse); 25069 (Rat)
  • Alternate Names:
    CD30 antigen; CD30; CD30KI-1; CD30L receptor; cytokine receptor CD30; D1S166EKi-1; Ki-1 antigen; Lymphocyte activation antigen CD30; TNFRSF8; tumor necrosis factor receptor superfamily member 8; tumor necrosis factor receptor superfamily, member 8
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Selective redox regulation of cytokine receptor signaling by extracellular thioredoxin-1.
    Authors: Schwertassek U, Balmer Y, Gutscher M, Weingarten L, Preuss M, Engelhard J, Winkler M, Dick TP
    EMBO J., 2007;26(13):3086-97.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
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Mouse IgG2B Isotype Control

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Mouse IgG2B Isotype Control

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Mouse IgG2B Isotype Control

Ctrl MAB0042 6
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

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Mouse IgG Antibody

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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

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Rat Anti-Mouse IgG2B APC-conjugated Antibody

Flow F0133 1  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
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Rat Anti-Mouse IgG2B PE-conjugated Antibody

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Mouse IgG2B Antibody

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Rat Anti-Mouse IgG2B Fluorescein-conjugated Antibody

Flow F0134 1
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