Human CD31/PECAM-1 Antibody

Catalog # Availability Size / Price Qty
AF806
AF806-SP
Detection of Human CD31/PECAM‑1 by Western Blot.
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Product Details
Citations (16)
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Human CD31/PECAM-1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human CD31 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse CD31 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human CD31
Extracellular domain
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
10 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human CD31/PECAM‑1 by Western Blot. View Larger

Detection of Human CD31/PECAM‑1 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human CD31/PECAM‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF806) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for CD31/PECAM‑1 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry CD31/PECAM‑1 in HUVECs. View Larger

CD31/PECAM‑1 in HUVECs. CD31/PECAM‑1 was detected in immersion fixed HUVEC human umbilical vein endothelial cells using 10 µg/mL Sheep Anti-Human CD31/PECAM‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF806) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Simple Western Detection of Human CD31/PECAM‑1 by Simple WesternTM. View Larger

Detection of Human CD31/PECAM‑1 by Simple WesternTM. Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line and HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for CD31/PECAM‑1 at approximately
173-186 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human CD31/PECAM‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF806) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD31/PECAM-1

The CD31 adhesion molecule, also known as PECAM-1, is expressed in large amounts on endothelial cells at intercellular junctions and on T cell subsets, and to a lesser extent on platelets and most other leukocytes such as monocytes and neutrophils. CD31 binds to itself homotypically, and also to the leukocyte integrin alpha v beta 3 heterotypically. CD31 is required for the transendothelial migration of leukocytes through intercellular junctions of vascular endothelial cells. CD31 has been found in human plasma, and the presence of this circulating isoform is suggested to modulate the transendothelial migration of leukocytes.

Long Name
Platelet Endothelial Cell Adhesion Molecule 1
Entrez Gene IDs
5175 (Human); 18613 (Mouse); 29583 (Rat)
Alternate Names
adhesion molecule; CD31 antigen; CD31; CD31/EndoCAM; endoCAM; FLJ34100; FLJ58394; GPIIA'; PECA1; PECAM1; PECAM-1; PECAM-1, CD31/EndoCAM; platelet endothelial cell adhesion molecule; platelet/endothelial cell adhesion molecule

Product Datasheets

Citations for Human CD31/PECAM-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

16 Citations: Showing 1 - 10
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  1. Leucine-Rich ?-2-Glycoprotein-1 (LRG-1) Expression in Retinoblastoma
    Authors: R Amer, L Tiosano, J Pe'er
    Invest. Ophthalmol. Vis. Sci., 2018;59(2):685-692.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  2. Tissue-engineered 3D melanoma model with blood and lymphatic capillaries for drug development
    Authors: J Bourland, J Fradette, FA Auger
    Sci Rep, 2018;8(1):13191.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  3. Tissue-engineered 3D human lymphatic microvascular network for in vitro studies of lymphangiogenesis
    Authors: L Gibot, T Galbraith, J Bourland, A Rogic, M Skobe, FA Auger
    Nat Protoc, 2017;12(5):1077-1088.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  4. Laminin-guided highly efficient endothelial commitment from human pluripotent stem cells
    Sci Rep, 2016;6(0):35680.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  5. Aberrant bispecific antibody pharmacokinetics linked to liver sinusoidal endothelium clearance mechanism in cynomolgus monkeys
    Authors: A Datta-Mann, JE Croy, L Schirtzing, S Torgerson, M Breyer, VJ Wroblewski
    MAbs, 2016;8(5):969-82.
  6. IGFBP-4 and -5 are expressed in first-trimester villi and differentially regulate the migration of HTR-8/SVneo cells.
    Authors: Crosley, Erin J, Dunk, Caroline, Beristain, Alexande, Christians, Julian K
    Reprod Biol Endocrinol, 2015;12(0):123.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  7. A BMP7 Variant Inhibits Tumor Angiogenesis In Vitro and In Vivo through Direct Modulation of Endothelial Cell Biology.
    Authors: Tate C, Mc Entire J, Pallini R, Vakana E, Wyss L, Blosser W, Ricci-Vitiani L, D'Alessandris Q, Morgante L, Giannetti S, Larocca L, Todaro M, Benfante A, Colorito M, Stassi G, De Maria R, Rowlinson S, Stancato L
    PLoS ONE, 2015;10(4):e0125697.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC-Fr
  8. TGF-beta1-induced EMT promotes targeted migration of breast cancer cells through the lymphatic system by the activation of CCR7/CCL21-mediated chemotaxis.
    Authors: Pang M, Georgoudaki A, Lambut L, Johansson J, Tabor V, Hagikura K, Jin Y, Jansson M, Alexander J, Nelson C, Jakobsson L, Betsholtz C, Sund M, Karlsson M, Fuxe J
    Oncogene, 2015;35(6):748-60.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  9. A method to assess target gene involvement in angiogenesis in vitro and in vivo using lentiviral vectors expressing shRNA.
    Authors: Blosser, Wayne, Vakana, Eliza, Wyss, Lisa V, Swearingen, Michelle, Stewart, Julie, Stancato, Louis, Tate, Courtney
    PLoS ONE, 2014;9(4):e96036.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  10. miR-27a regulates endothelial differentiation of breast cancer stem like cells.
    Authors: Tang, W, Yu, F, Yao, H, Cui, X, Jiao, Y, Lin, L, Chen, J, Yin, D, Song, E, Liu, Q
    Oncogene, 2014;33(20):2629-38.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  11. Consumption of a Western-style diet during pregnancy impairs offspring islet vascularization in a Japanese macaque model.
    Authors: Pound L, Comstock S, Grove K
    Am J Physiol Endocrinol Metab, 2014;307(1):E115-23.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Types: Whole Tissue
    Applications: IHC-P
  12. Type IV collagen stimulates pancreatic cancer cell proliferation, migration, and inhibits apoptosis through an autocrine loop.
    Authors: Ohlund D, Franklin O, Lundberg E, Lundin C, Sund M
    BMC Cancer, 2013;13(0):154.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  13. Maternal high-fat diet impacts endothelial function in nonhuman primate offspring.
    Authors: Fan, L, Lindsley, S R, Comstock, S M, Takahashi, D L, Evans, A E, He, G-W, Thornburg, K L, Grove, K L
    Int J Obes (Lond), 2013;37(2):254-62.
    Species: Primate - Macaca fuscata (Japanese Macaque)
    Sample Types: Whole Tissue
    Applications: IHC-P
  14. Signaling hierarchy regulating human endothelial cell development.
    Authors: Kelly MA, Hirschi KK
    Arterioscler. Thromb. Vasc. Biol., 2009;29(5):718-24.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  15. Proteomic analysis reveals presence of platelet microparticles in endothelial progenitor cell cultures.
    Authors: Prokopi M, Pula G, Mayr U, Devue C, Gallagher J, Xiao Q, Boulanger CM, Westwood N, Urbich C, Willeit J, Steiner M, Breuss J, Xu Q, Kiechl S, Mayr M
    Blood, 2009;114(3):723-32.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  16. Recapitulation of pancreatic neuroendocrine tumors in human multiple endocrine neoplasia type I syndrome via Pdx1-directed inactivation of Men1.
    Authors: Shen HC, He M, Powell A, Adem A, Lorang D, Heller C, Grover AC, Ylaya K, Hewitt SM, Marx SJ, Spiegel AM, Libutti SK
    Cancer Res., 2009;69(5):1858-66.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr

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