Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Bovine, Primate, Primate - Macaca fuscata (Japanese Macaque), Primate - Macaca mulatta (Rhesus Macaque), Rabbit, Transgenic Mouse, Xenograft

Applications

Validated:

Knockout Validated, Immunohistochemistry, Western Blot, Immunocytochemistry, Simple Western

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunocytochemistry, ELISA Capture, FACS, Flourescense Microscopy

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all CD31/PECAM-1 Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human CD31
Extracellular domain

Specificity

Detects human CD31 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse CD31 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human CD31/PECAM‑1 Antibody

Detection of Human CD31/PECAM-1 antibody by Western Blot.

Detection of Human CD31/PECAM‑1 by Western Blot.

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human CD31/PECAM-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF806) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). A specific band was detected for CD31/PECAM-1 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
CD31/PECAM-1 antibody in HUVECs by Immunocytochemistry (ICC).

CD31/PECAM‑1 in HUVECs.

CD31/PECAM-1 was detected in immersion fixed HUVEC human umbilical vein endothelial cells using 10 µg/mL Sheep Anti-Human CD31/PECAM-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF806) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of Human CD31/PECAM‑1 by Simple WesternTM.

Left: Simple Western lane view shows lysates of HUVEC human umbilical vein endothelial cells, loaded at 0.1 mg/ml. A specific band was detected for CD31/PECAM‑1 at approximately 155 kDa (as indicated) using both 10 µg/ml and 50 µg/ml of Sheep Anti-Human CD31/PECAM‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF806) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016) in Milk-free Antibody Diluent (Catalog # 043-524). This experiment was conducted under reducing conditions and using the 12-230kDa separation system. Right: Simple Western electropherogram showing the same Sheep Anti-Human CD31/PECAM‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF806) tested at 10 µg/ml (blue line) and 50 µg/ml (green line) in the HUVEC human umbilical vein endothelial cells.

CD31/PECAM‑1 Specificity is Shown by Immunocytochemistry in Knockout Cell Line.

CD31/PECAM‑1 was detected in immersion fixed THP‑1 human acute monocytic leukemia cell line but is not detected in CD31/PECAM‑1 knockout (KO) THP‑1 human cell line using Sheep Anti-Human CD31/PECAM‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF806) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; NL010) and counterstained with DAPI (blue). Specific staining was localized to cell membrane. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Detection of CD31/PECAM‑1 in human liver.

CD31/PECAM‑1 was detected in immersion fixed paraffin-embedded sections of human liver using Sheep Anti-Human CD31/PECAM‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF806) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to endothelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Detection of CD31/PECAM‑1 in human liver.

CD31/PECAM‑1 was detected in immersion fixed paraffin-embedded sections of human liver using Sheep Anti-Human CD31/PECAM‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF806) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to endothelial cells and sinusoids. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Detection of Human CD31/PECAM-1 by Immunocytochemistry/Immunofluorescence

Detection of Human CD31/PECAM-1 by Immunocytochemistry/Immunofluorescence

Expression pattern of integrin receptors and type IV collagen in normal pancreatic tissue. A. H&E staining of normal exocrine pancreas with acini structures and a duct in the insert. The area shown is representative for all panels in B. B. Merged immunofluorescence staining of integrin alpha 1, alpha 2, and beta 1 (in red), and type IV collagen (green in upper row), or the endothelial marker CD31 (green in lower row). Type IV collagen is present in the vascular-, ductal-, and acini-BMs. Integrin alpha 1 is only expressed in endothelial cells and integrin alpha 2 only in the ductal epithelium. Integrin beta 1 is found in the ductal-, endothelial-, and at the base of the acini-cells. Arrows indicate examples of colocalization. Magnification x40 in all panels. Cell nuclei are stained by DAPI (in blue). Image collected and cropped by CiteAb from the following publication (https://bmccancer.biomedcentral.com/articles/10.1186/1471-2407-13-154), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human CD31/PECAM‑1 Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed HUVEC human umbilical vein endothelial cells

Immunohistochemistry

5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human liver

Knockout Validated

CD31/PECAM‑1 was detected in immersion fixed THP‑1 human acute monocytic leukemia cell line but is not detected in CD31/PECAM‑1 knockout (KO) THP‑1 human cell line.

Simple Western

10-50 µg/mL
Sample: HUVEC human umbilical vein endothelial cells

Western Blot

1 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line

Reviewed Applications

Read 3 reviews rated 4.7 using AF806 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD31/PECAM-1

The CD31 adhesion molecule, also known as PECAM-1, is expressed in large amounts on endothelial cells at intercellular junctions and on T cell subsets, and to a lesser extent on platelets and most other leukocytes such as monocytes and neutrophils. CD31 binds to itself homotypically, and also to the leukocyte integrin alpha v beta 3 heterotypically. CD31 is required for the transendothelial migration of leukocytes through intercellular junctions of vascular endothelial cells. CD31 has been found in human plasma, and the presence of this circulating isoform is suggested to modulate the transendothelial migration of leukocytes.

Long Name

Platelet Endothelial Cell Adhesion Molecule 1

Alternate Names

CD31, EndoCAM, PECA1, PECAM-1, PECAM1

Entrez Gene IDs

5175 (Human); 18613 (Mouse); 29583 (Rat)

Gene Symbol

PECAM1

Additional CD31/PECAM-1 Products

Product Documents for Human CD31/PECAM‑1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human CD31/PECAM‑1 Antibody

For research use only

Citations for Human CD31/PECAM‑1 Antibody

Customer Reviews for Human CD31/PECAM‑1 Antibody (3)

4.7 out of 5
3 Customer Ratings
5 Stars
67%
4 Stars
33%
3 Stars
0%
2 Stars
0%
1 Stars
0%

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Customer Images

Showing  1 - 3 of 3 reviews Showing All
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  • Human CD31/PECAM-1 Antibody
    Name: Ivan Osinnii
    Application: Immunohistochemistry-Frozen
    Sample Tested: Human Colon and colon
    Species: Human
    Verified Customer | Posted 08/21/2023
    IF of healthy human colon 8 micrometer cryosections fixed with 4% PFA. 2 different secondary antibodies were used. Please see Primary antibody concentrations and fluorescence channels designation on the image.
    Immunofluorescence on freshly-frozen PFA-fixed cryosections
    Human CD31/PECAM‑1 Antibody AF806
  • Human CD31/PECAM-1 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Cell Lysates
    Species: Human
    Verified Customer | Posted 05/27/2022
    Human CD31/PECAM‑1 Antibody AF806
  • Human CD31/PECAM-1 Antibody
    Name: Karina Kinghorn
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: HUVEC human umbilical vein endothelial cells
    Species: Human
    Verified Customer | Posted 08/04/2021
    Human CD31/PECAM‑1 Antibody AF806

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