Human CD31/PECAM-1 Antibody

Catalog # Availability Size / Price Qty
AF806
AF806-SP
Detection of Human CD31/PECAM‑1 by Western Blot.
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Product Details
Citations (31)
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Reviews (2)

Human CD31/PECAM-1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human CD31 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse CD31 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human CD31
Extracellular domain
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
10 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
See below
Knockout Validated
CD31/PECAM‑1 was detected in immersion fixed THP‑1 human acute monocytic leukemia cell line but is not detected in CD31/PECAM‑1 knockout (KO) THP‑1 human cell line.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human CD31/PECAM-1 antibody by Western Blot. View Larger

Detection of Human CD31/PECAM‑1 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human CD31/PECAM-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF806) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for CD31/PECAM-1 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry CD31/PECAM-1 antibody in HUVECs by Immunocytochemistry (ICC). View Larger

CD31/PECAM‑1 in HUVECs. CD31/PECAM-1 was detected in immersion fixed HUVEC human umbilical vein endothelial cells using 10 µg/mL Sheep Anti-Human CD31/PECAM-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF806) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Simple Western Detection of Human CD31/PECAM-1 antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human CD31/PECAM‑1 by Simple WesternTM. Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line and HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for CD31/PECAM-1 at approximately 173-186 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human CD31/PECAM-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF806) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Knockout Validated View Larger

CD31/PECAM‑1 Specificity is Shown by Immunocytochemistry in Knockout Cell Line. CD31/PECAM‑1 was detected in immersion fixed THP‑1 human acute monocytic leukemia cell line but is not detected in CD31/PECAM‑1 knockout (KO) THP‑1 human cell line using Sheep Anti-Human CD31/PECAM‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF806) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; NL010) and counterstained with DAPI (blue). Specific staining was localized to cell membrane. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Immunohistochemistry View Larger

Detection of CD31/PECAM‑1 in human liver. CD31/PECAM‑1 was detected in immersion fixed paraffin-embedded sections of human liver using Sheep Anti-Human CD31/PECAM‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF806) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to endothelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Immunohistochemistry View Larger

Detection of CD31/PECAM‑1 in human liver. CD31/PECAM‑1 was detected in immersion fixed paraffin-embedded sections of human liver using Sheep Anti-Human CD31/PECAM‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF806) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to endothelial cells and sinusoids. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD31/PECAM-1

The CD31 adhesion molecule, also known as PECAM-1, is expressed in large amounts on endothelial cells at intercellular junctions and on T cell subsets, and to a lesser extent on platelets and most other leukocytes such as monocytes and neutrophils. CD31 binds to itself homotypically, and also to the leukocyte integrin alpha v beta 3 heterotypically. CD31 is required for the transendothelial migration of leukocytes through intercellular junctions of vascular endothelial cells. CD31 has been found in human plasma, and the presence of this circulating isoform is suggested to modulate the transendothelial migration of leukocytes.

Long Name
Platelet Endothelial Cell Adhesion Molecule 1
Entrez Gene IDs
5175 (Human); 18613 (Mouse); 29583 (Rat)
Alternate Names
adhesion molecule; CD31 antigen; CD31; CD31/EndoCAM; EndoCAM; FLJ34100; FLJ58394; GPIIA'; PECA1; PECAM1; PECAM-1; PECAM-1, CD31/EndoCAM; platelet endothelial cell adhesion molecule; platelet endothelial cell adhesion molecule-1; platelet/endothelial cell adhesion molecule

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Citations for Human CD31/PECAM-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

31 Citations: Showing 1 - 10
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  1. A Personalized Glomerulus Chip Engineered from Stem Cell-Derived Epithelium and Vascular Endothelium
    Authors: Y Roye, R Bhattachar, X Mou, Y Zhou, MA Burt, S Musah
    Micromachines, 2021;12(8):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  2. Key molecular alterations in endothelial cells in human glioblastoma uncovered through single-cell RNA sequencing
    Authors: Y Xie, L He, R Lugano, Y Zhang, H Cao, Q He, M Chao, B Liu, Q Cao, J Wang, Y Jiao, Y Hu, L Han, Y Zhang, H Huang, L Uhrbom, C Betsholtz, L Wang, A Dimberg, L Zhang
    JCI Insight, 2021;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  3. Mechanisms of angiogenic incompetence in Hutchinson-Gilford progeria via downregulation of endothelial NOS
    Authors: YG Gete, LW Koblan, X Mao, M Trappio, B Mahadik, JP Fisher, DR Liu, K Cao
    Aging Cell, 2021;0(0):e13388.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. 3D iPSC modeling of the retinal pigment epithelium-choriocapillaris complex identifies factors involved in the pathology of macular degeneration
    Authors: KV Manian, CA Galloway, S Dalvi, AA Emanuel, JA Mereness, W Black, L Winschel, C Soto, Y Li, Y Song, W DeMaria, A Kumar, I Slukvin, MP Schwartz, WL Murphy, B Anand-Apte, M Chung, DSW Benoit, R Singh
    Cell Stem Cell, 2021;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  5. Generation, functional analysis and applications of isogenic three-dimensional self-aggregating cardiac microtissues from human pluripotent stem cells
    Authors: G Campostrin, V Meraviglia, E Giacomelli, RWJ van Helden, L Yiangou, RP Davis, M Bellin, VV Orlova, CL Mummery
    Nature Protocols, 2021;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. SARS-CoV-2 Infects Syncytiotrophoblast and Activates Inflammatory Responses in the Placenta
    Authors: LB Argueta, LA Lacko, Y Bram, T Tada, L Carrau, T Zhang, S Uhl, BC Lubor, V Chandar, C Gil, W Zhang, B Dodson, J Bastiaans, M Prabhu, CM Salvatore, YJ Yang, RN Baergen, BR tenOever, NR Landau, S Chen, RE Schwartz, H Stuhlmann
    bioRxiv : the preprint server for biology, 2021;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  7. Reconstruction of the human blood-brain barrier in vitro reveals a pathogenic mechanism of APOE4 in pericytes
    Authors: JW Blanchard, M Bula, J Davila-Vel, LA Akay, L Zhu, A Frank, MB Victor, JM Bonner, H Mathys, YT Lin, T Ko, DA Bennett, HP Cam, M Kellis, LH Tsai
    Nat. Med., 2020;26(6):952-963.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  8. Abnormal Microvascular Architecture, Fibrosis, and Pericyte Characteristics in the Calf Muscle of Peripheral Artery Disease Patients with Claudication and Critical Limb Ischemia
    Authors: CJ Mietus, TJ Lackner, PS Karvelis, GT Willcockso, CM Shields, NG Lambert, P Koutakis, MA Fuglestad, H Hernandez, GR Haynatzki, JKS Kim, HK DeSpiegela, II Pipinos, GP Casale
    J Clin Med, 2020;9(8):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  9. Conditioned medium produced by fibroblasts cultured in low oxygen pressure allows the formation of highly structured capillary-like networks in fibrin gels
    Authors: C Caneparo, C Baratange, S Chabaud, S Bolduc
    Sci Rep, 2020;10(1):9291.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  10. Multi-layered Spatial Transcriptomics Identify Secretory Factors Promoting Human Hematopoietic Stem Cell Development
    Authors: EI Crosse, S Gordon-Key, S Rybtsov, A Binagui-Ca, H Felchle, NC Nnadi, K Kirschner, T Chandra, S Tamagno, DJ Webb, F Rossi, RA Anderson, A Medvinsky
    Cell Stem Cell, 2020;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  11. Abnormal Microvascular Architecture, Fibrosis, and Pericyte Characteristics in the Calf Muscle of Peripheral Artery Disease Patients with Claudication and Critical Limb Ischemia
    Authors: CJ Mietus, TJ Lackner, PS Karvelis, GT Willcockso, CM Shields, NG Lambert, P Koutakis, MA Fuglestad, H Hernandez, GR Haynatzki, JKS Kim, HK DeSpiegela, II Pipinos, GP Casale
    J Clin Med, 2020;9(8):.
    Species: Bovine
    Sample Types: Whole Cells
    Applications: ICC
  12. Human-iPSC-Derived Cardiac Stromal Cells Enhance Maturation in 3D Cardiac Microtissues and Reveal Non-cardiomyocyte Contributions to Heart Disease
    Authors: E Giacomelli, V Meraviglia, G Campostrin, A Cochrane, X Cao, RWJ van Helden, A Krotenberg, M Mircea, S Kostidis, RP Davis, BJ van Meer, CR Jost, AJ Koster, H Mei, DG Míguez, AA Mulder, M Ledesma-Te, G Pompilio, L Sala, DCF Salvatori, RC Slieker, E Sommariva, AAF de Vries, M Giera, S Semrau, LGJ Tertoolen, VV Orlova, M Bellin, CL Mummery
    Cell Stem Cell, 2020;26(6):862-879.e11.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  13. Maternal Western-style diet affects offspring islet composition and function in a non-human primate model of maternal over-nutrition
    Authors: JM Elsakr, JC Dunn, K Tennant, SK Zhao, K Kroeten, RC Pasek, DL Takahashi, TA Dean, DR Velez Edwa, CE McCurdy, KM Aagaard, AC Powers, JE Friedman, P Kievit, M Gannon
    Mol Metab, 2019;0(0):.
    Species: Primate - Macaca fuscata (Japanese Macaque)
    Sample Types: Whole Tissue
    Applications: IHC-P
  14. Mineral deposition and vascular invasion of hydroxyapatite reinforced collagen scaffolds seeded with human adipose-derived stem cells
    Authors: HE Weiss-Bilk, MJ Meagher, JA Gargac, GL Niebur, RK Roeder, DR Wagner
    Biomater Res, 2019;23(0):15.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flourescense Microscopy
  15. Circulating Vascular Basement Membrane Fragments are Associated with the Diameter of the Abdominal Aorta and Their Expression Pattern is Altered in AAA Tissue
    Authors: M Holsti, A Wanhainen, C Lundin, M Björck, G Tegler, J Svensson, M Sund
    Eur J Vasc Endovasc Surg, 2018;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  16. Tissue-engineered 3D melanoma model with blood and lymphatic capillaries for drug development
    Authors: J Bourland, J Fradette, FA Auger
    Sci Rep, 2018;8(1):13191.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  17. Leucine-Rich ?-2-Glycoprotein-1 (LRG-1) Expression in Retinoblastoma
    Authors: R Amer, L Tiosano, J Pe'er
    Invest. Ophthalmol. Vis. Sci., 2018;59(2):685-692.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  18. Tissue-engineered 3D human lymphatic microvascular network for in vitro studies of lymphangiogenesis
    Authors: L Gibot, T Galbraith, J Bourland, A Rogic, M Skobe, FA Auger
    Nat Protoc, 2017;12(5):1077-1088.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  19. Laminin-guided highly efficient endothelial commitment from human pluripotent stem cells
    Sci Rep, 2016;6(0):35680.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  20. Aberrant bispecific antibody pharmacokinetics linked to liver sinusoidal endothelium clearance mechanism in cynomolgus monkeys
    Authors: A Datta-Mann, JE Croy, L Schirtzing, S Torgerson, M Breyer, VJ Wroblewski
    MAbs, 2016;8(5):969-82.
  21. TGF-beta1-induced EMT promotes targeted migration of breast cancer cells through the lymphatic system by the activation of CCR7/CCL21-mediated chemotaxis.
    Authors: Pang M, Georgoudaki A, Lambut L, Johansson J, Tabor V, Hagikura K, Jin Y, Jansson M, Alexander J, Nelson C, Jakobsson L, Betsholtz C, Sund M, Karlsson M, Fuxe J
    Oncogene, 2015;35(6):748-60.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  22. A BMP7 Variant Inhibits Tumor Angiogenesis In Vitro and In Vivo through Direct Modulation of Endothelial Cell Biology.
    Authors: Tate C, Mc Entire J, Pallini R, Vakana E, Wyss L, Blosser W, Ricci-Vitiani L, D'Alessandris Q, Morgante L, Giannetti S, Larocca L, Todaro M, Benfante A, Colorito M, Stassi G, De Maria R, Rowlinson S, Stancato L
    PLoS ONE, 2015;10(4):e0125697.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC-Fr
  23. IGFBP-4 and -5 are expressed in first-trimester villi and differentially regulate the migration of HTR-8/SVneo cells.
    Authors: Crosley, Erin J, Dunk, Caroline, Beristain, Alexande, Christians, Julian K
    Reprod Biol Endocrinol, 2015;12(0):123.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  24. Consumption of a Western-style diet during pregnancy impairs offspring islet vascularization in a Japanese macaque model.
    Authors: Pound L, Comstock S, Grove K
    Am J Physiol Endocrinol Metab, 2014;307(1):E115-23.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Types: Whole Tissue
    Applications: IHC-P
  25. miR-27a regulates endothelial differentiation of breast cancer stem like cells.
    Authors: Tang, W, Yu, F, Yao, H, Cui, X, Jiao, Y, Lin, L, Chen, J, Yin, D, Song, E, Liu, Q
    Oncogene, 2014;33(20):2629-38.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  26. A method to assess target gene involvement in angiogenesis in vitro and in vivo using lentiviral vectors expressing shRNA.
    Authors: Blosser, Wayne, Vakana, Eliza, Wyss, Lisa V, Swearingen, Michelle, Stewart, Julie, Stancato, Louis, Tate, Courtney
    PLoS ONE, 2014;9(4):e96036.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  27. Maternal high-fat diet impacts endothelial function in nonhuman primate offspring.
    Authors: Fan, L, Lindsley, S R, Comstock, S M, Takahashi, D L, Evans, A E, He, G-W, Thornburg, K L, Grove, K L
    Int J Obes (Lond), 2013;37(2):254-62.
    Species: Primate - Macaca fuscata (Japanese Macaque)
    Sample Types: Whole Tissue
    Applications: IHC-P
  28. Type IV collagen stimulates pancreatic cancer cell proliferation, migration, and inhibits apoptosis through an autocrine loop.
    Authors: Ohlund D, Franklin O, Lundberg E, Lundin C, Sund M
    BMC Cancer, 2013;13(0):154.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  29. Recapitulation of pancreatic neuroendocrine tumors in human multiple endocrine neoplasia type I syndrome via Pdx1-directed inactivation of Men1.
    Authors: Shen HC, He M, Powell A, Adem A, Lorang D, Heller C, Grover AC, Ylaya K, Hewitt SM, Marx SJ, Spiegel AM, Libutti SK
    Cancer Res., 2009;69(5):1858-66.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  30. Proteomic analysis reveals presence of platelet microparticles in endothelial progenitor cell cultures.
    Authors: Prokopi M, Pula G, Mayr U, Devue C, Gallagher J, Xiao Q, Boulanger CM, Westwood N, Urbich C, Willeit J, Steiner M, Breuss J, Xu Q, Kiechl S, Mayr M
    Blood, 2009;114(3):723-32.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  31. Signaling hierarchy regulating human endothelial cell development.
    Authors: Kelly MA, Hirschi KK
    Arterioscler. Thromb. Vasc. Biol., 2009;29(5):718-24.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

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Reviews for Human CD31/PECAM-1 Antibody

Average Rating: 4.5 (Based on 2 Reviews)

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Human CD31/PECAM-1 Antibody
By Anonymous on 05/27/2022
Application: WB Sample Tested: Cell Lysates Species: Human

Human CD31/PECAM-1 Antibody
By Karina Kinghorn on 08/04/2021
Application: ICC/IF Sample Tested: HUVEC human umbilical vein endothelial cells Species: Human