Human CD34 Antibody

  (2 citations)
(1 Review)
    
Datasheet / CoA / SDS
Product Details
Citations (2)
FAQs
Supplemental Products
Reviews
  • Species Reactivity
    Human
  • Specificity
    Detects human CD34 in direct ELISAs. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse CD34 and recombinant rat CD34 is observed.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Chinese hamster ovary cell line CHO-derived recombinant human CD34 Ser32-Thr290 Accession # P28906
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Immunohistochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Immunohistochemistry
CD34 in Human Liver. CD34 was detected in immersion fixed paraffin-embedded sections of human liver using Sheep Anti-Human CD34 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7227) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to endothelial cells in vasculature. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.2 mg/mL.
    Reconstitution Buffer Available
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
      12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Background: CD34
CD34 is a 105-115 kDa member of the CD34/podocalyxin family of molecules. It is a sialomucin type glycoprotein, and presents carbohydrate to selectins during cell migration. CD34 is found on mast cells, eosinophils, vascular endothelial cells, stem cells and renal mesangial cells. Mature human CD34 is a 354 amino acid (aa) type I transmembrane protein (aa 32-385). It contains a 259 aa extracellular region (aa 35-287) with utilized N- and O-linked glycosylation sites, and a 74 aa cytoplasmic domain that may undergo Tyr phosphorylation. There is one splice variant that shows a four aa substitution for aa 325-385. Human CD34 can undergo membrane cleavage by bacterial proteases to generate 30-40 kDa soluble fragments. And notably, desialylated CD34 shows a 40 kDa increase in MW (to 150 kDa) when run in SDS-PAGE. Over aa 32-290, human CD34 shares 56% aa identity with mouse CD34.
  • Entrez Gene IDs:
    947 (Human); 12490 (Mouse); 305081 (Rat); 397160 (Porcine); 415130 (Canine)
  • Alternate Names:
    CD34 antigenhematopoietic progenitor cell antigen CD34; CD34 molecule; CD34; HPCA1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Species
Applications
Sample Type
  1. Multicolor quantitative confocal imaging cytometry
    Authors: DL Coutu, KD Kokkaliari, L Kunz, T Schroeder
    Nat. Methods, 2018;15(1):39-46.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  2. Effects of negative-pressure wound therapy combinedwith microplasma on treating wounds of ulcer and the expression of heat shock protein 90
    Authors: Z Li, Q Wang, W Mi, M Han, F Gao, G Niu, Y Ma
    Exp Ther Med, 2017;13(5):2211-2216.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC-P

FAQs

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Cell and Tissue Staining Kits
Description Application Cat# Citations Images  

Anti-Sheep HRP-DAB Cell & Tissue Staining Kit

CTS019 2
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Immunohistochemistry Reagents
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Antigen Retrieval Reagent-Universal

CTS015 10
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Antigen Retrieval Reagent-Basic

CTS013 5
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Mounting Medium

CTS011 4
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Antigen Retrieval Reagent Sampler (50 mL each)

CTS016 1
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Antigen Retrieval Reagent-Acidic

CTS014
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VisUCyte Antigen Retrieval Reagent-Acidic

VCTS022
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NorthernLights Guard Mounting Media

NL996
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VisUCyte Antigen Retrieval Reagent-Universal

VCTS023
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VisUCyte Antigen Retrieval Reagent-Basic

VCTS021
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Isotype Controls
Description Application Cat# Citations Images  

Normal Sheep IgG Control

Ctrl 5-001-A 11
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Reconstitution Buffers
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Reconstitution Buffer 1 (PBS)

RB01
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Secondary Antibodies
Description Application Cat# Citations Images  

Sheep IgG HRP-conjugated Antibody

WB , Simple Western HAF016 18  
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Donkey Anti-Sheep IgG NorthernLights™ NL557-conjugated Antibody

Flow , IHC , ICC NL010 3
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Donkey Anti-Sheep IgG NorthernLights™ NL637-conjugated Antibody

Flow , IHC , ICC NL011 5
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Donkey Anti-Sheep IgG NorthernLights™ NL493-conjugated Antibody

Flow , IHC , ICC NL012 3
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Sheep IgG (H+L) PE-conjugated Antibody

Flow F0126  
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Donkey Anti-Sheep IgG Biotinylated Antibody

WB BAF016 2
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Sheep IgG (H+L) APC-conjugated Antibody

Flow F0127  
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Sheep IgG VisUCyte HRP Polymer Antibody

IHC VC006  
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Donkey Anti-Sheep IgG (H+L) PerCP-conjugated Antibody

Flow F0128
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Average Rating: 5 (Based on 1 review)

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Read what people are saying who have used Human CD34 Antibody.

We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: Immunohistochemistry.

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Images Ratings Applications Species Reviewed By Date Details
Excellent
 IHC Human Anonymous 10/31/2017
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Summary

Application Immunohistochemistry
Sample Tested Lymph node tissue
Species Human

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