Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all CD34 Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human CD34
Ser32-Thr290
Accession # P28906

Specificity

Detects human CD34 in direct ELISAs. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse CD34 and recombinant rat CD34 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human CD34 Antibody

Detection of Human CD34 antibody by Western Blot.

Detection of Human CD34 by Western Blot.

Western blot shows lysates of human testies tissue and human thymus tissue. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human CD34 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7227) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CD34 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
CD34 antibody in Human Liver by Immunohistochemistry (IHC-P).

CD34 in Human Liver.

CD34 was detected in immersion fixed paraffin-embedded sections of human liver using Sheep Anti-Human CD34 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7227) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to endothelial cells in vasculature. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Human CD34 by Immunohistochemistry

Detection of Human CD34 by Immunohistochemistry

sAng-2 concentration positively relates with Ang-2 expression on the epithelia and MVD in cervical tissues.Representative immunohistochemical staining of Ang-1 (A), Ang-2 (B) and CD34 (C) in 25 cervical cancer tissue specimens and 10 normal controls. Black arrows denote positively stained epithelial cells, whereas red arrows denote positively staining endothelial cells, all appearing brown. Scale bar, 20 µm. (D) sAng-2 is significantly higher in the patients with positive Ang-2 expression on cervix epithelia than those with negative Ang-2 expression. The scatter diagrams show the correlations of sAng-1 (E), sAng-2 (F) and sAng-1/ sAng-2 ratio (G) to MVD in the 35 cervical tissue specimens. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28584715), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human CD34 Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human liver

Western Blot

0.5 µg/mL
Sample: Human testies tissue and Human thymus tissue

Reviewed Applications

Read 1 review rated 5 using AF7227 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD34

CD34 is a 105-115 kDa member of the CD34/podocalyxin family of molecules. It is a sialomucin type glycoprotein, and presents carbohydrate to selectins during cell migration. CD34 is found on mast cells, eosinophils, vascular endothelial cells, stem cells and renal mesangial cells. Mature human CD34 is a 354 amino acid (aa) type I transmembrane protein (aa 32-385). It contains a 259 aa extracellular region (aa 35-287) with utilized N- and O-linked glycosylation sites, and a 74 aa cytoplasmic domain that may undergo Tyr phosphorylation. There is one splice variant that shows a four aa substitution for aa 325-385. Human CD34 can undergo membrane cleavage by bacterial proteases to generate 30-40 kDa soluble fragments. And notably, desialylated CD34 shows a 40 kDa increase in MW (to 150 kDa) when run in SDS-PAGE. Over aa 32-290, human CD34 shares 56% aa identity with mouse CD34.

Alternate Names

CD34, HPCA1

Entrez Gene IDs

947 (Human); 12490 (Mouse); 305081 (Rat); 397160 (Porcine); 415130 (Canine)

Gene Symbol

CD34

UniProt

P28906

Additional CD34 Products

Product Documents for Human CD34 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human CD34 Antibody

For research use only

Citations for Human CD34 Antibody

Customer Reviews for Human CD34 Antibody (1)

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  • Human CD34 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Lymph node tissue
    Species: Human
    Verified Customer | Posted 10/31/2017

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Protocols

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