Human CD63 Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB50482-100
MAB50482-SP
Detection of Human CD63 by Western Blot.
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Product Details
Citations (1)
FAQs
Supplemental Products
Reviews (1)

Human CD63 Antibody Summary

Species Reactivity
Human
Specificity
Detects human CD63 in direct ELISAs.
Source
Recombinant Monoclonal Rabbit IgG Clone # 2585J
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Chinese Hamster Ovary cell line CHO-derived human CD63
Ala103-Val203
Accession # P08962-1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
Simple Western
20 µg/mL
See below
Immunohistochemistry
0.3-25 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human CD63 antibody by Western Blot. View Larger

Detection of Human CD63 by Western Blot. Western blot shows lysates of human platelets. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human CD63 Monoclonal Antibody (Catalog # MAB50482) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for CD63 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry CD63 antibody in Human Lung Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

CD63 in Human Lung Cancer Tissue. CD63 was detected in immersion fixed paraffin-embedded sections of human lung cancer tissue using Rabbit Anti-Human CD63 Monoclonal Antibody (Catalog # MAB50482) at 0.3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell membrane in sperm cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Intracellular Staining by Flow Cytometry Detection of CD63 antibody in Human PBMC antibody by Flow Cytometry. View Larger

Detection of CD63 in Human PBMC by Flow Cytometry. Human PBMC were stained with Rabbit Anti-Human CD63 Monoclonal Antibody (Catalog # MAB50482, filled histogram) or isotype control antibody (Catalog # MAB1050, open histogram), followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3/Transcription Factor Fixation & Perm Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

Simple Western Detection of Human CD63 antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human CD63 by Simple WesternTM. Simple Western lane view shows lysates of human platelets, loaded at 0.2 mg/mL. A specific band was detected for CD63 at approximately 59 kDa (as indicated) using 20 µg/mL of Rabbit Anti-Human CD63 Monoclonal Antibody (Catalog # MAB50482). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD63

CD63, also known as LAMP-3 or ME491 (melanoma-associated antigen), is a 30-60 kDa member of the tetraspanin superfamily of protein trafficking proteins. CD63 is ubiquitously expressed and found in late endocytic vesicles, but following cell activation is also present on the plasma membrane. Interaction of CD63 with other membrane proteins or adaptors regulates cell activities such as adhesion, migration and degranulation. Extracellular regions of human CD63 share 67% and 65% amino acid sequence identity with mouse and rat CD63, respectively.

Entrez Gene IDs
967 (Human); 12512 (Mouse); 29186 (Rat); 474391 (Canine)
Alternate Names
CD_antigen: CD63; CD63 antigen (melanoma 1 antigen); CD63 antigen; CD63 molecule; CD63; Granulophysin; Lamp-3; Lysosomal-associated membrane protein 3; lysosome-associated membrane glycoprotein 3; ME491; melanoma 1 antigen; Melanoma-associated antigen ME491; melanoma-associated antigen MLA1; MLA1; Ocular melanoma-associated antigen; OMA81H; tetraspanin-30; Tspan30; tspan-30

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Citation for Human CD63 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Extracellular vesicle-derived long non-coding RNA as circulating biomarkers for endometriosis
    Authors: S Shan, Y Yang, J Jiang, B Yang, Y Yang, F Sun, J Zhang, Y Lin, H Xu
    Reproductive biomedicine online, 2021;0(0):.
    Species: Human
    Sample Types: Extracellular Vesicles
    Applications: Cell Isolation

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Human CD63 Antibody
By Anonymous on 11/25/2021
Application: IHC Sample Tested: Uterine epithelium Species: Human