Human CGRP Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
CGRP in Human Dorsal Root Ganglion.
2 Images
Product Details
Supplemental Products

Human CGRP Antibody Summary

Species Reactivity
Detects human CGRP in direct ELISA.
Recombinant Monoclonal Rabbit IgG Clone # 2413G
Protein A or G purified from cell culture supernatant
Human CGRP synthetic peptide (Tocris Catalog # 3012)
Accession # P06881-1
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
0.5 µg/mL
TT human medullary thyroid cancer cell line
3-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry CGRP antibody in Human Dorsal Root Ganglion by Immunohistochemistry (IHC-P). View Larger

CGRP in Human Dorsal Root Ganglion. CGRP was detected in immersion fixed paraffin-embedded sections of human dorsal root ganglion using Rabbit Anti-Human CGRP Monoclonal Antibody (Catalog # MAB83503) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Western Blot View Larger

Detection of Human CGRP by Western Blot. Western blot shows lysates of TT human medullary thyroid cancer cell line. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human CGRP Monoclonal Antibody (Catalog # MAB83503) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for CGRP at approximately 8 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CGRP1

Calcitonin gene-related peptide or CGRP is a 37-amino acid neuropeptide formed by alternative splicing of the calcitonin/CGRP gene. CGRP is a member of the calcitonin family of peptides consisting of calcitonin, amylin, adrenomedullin, adrenomedullin 2 (intermedin) and calcitonin‑receptor‑stimulating peptide. CGRP is produced in both peripheral and central neurons and induces vasodilation of a variety of vessels including the coronary, cerebral and systemic vasculature. Its abundance in the CNS also points toward a neurotransmitter or neuromodulator role.

Long Name
Calcitonin Gene Related Peptide I
Entrez Gene IDs
796 (Human)
Alternate Names
a-CGRP; alpha-CGRP; alpha-type CGRP; CALCA; calcitonin gene-related peptide I; calcitonin/calcitonin-related polypeptide, alpha; calcitonin; calcitonin gene-related peptide 1; CGRP 1; CGRP alpha; CGRP I; CGRP1; CGRP-I

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