Human Chordin-like 2/CHRDL2 Antibody

Catalog # Availability Size / Price Qty
AF2448
AF2448-SP
Chordin-like 2/CHRDL2 in Human Breast Cancer Tissue.
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Human Chordin-like 2/CHRDL2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Chordin-like 2/CHRDL2 in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Chordin-like 2/CHRDL2
Arg28-Thr451
Accession # NP_056239
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Chordin-Like 2/CHRDL2
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunohistochemistry Chordin-like 2/CHRDL2 in Human Breast Cancer Tissue. View Larger

Chordin-like 2/CHRDL2 in Human Breast Cancer Tissue. Chordin-like 2/CHRDL2 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using 15 µg/mL Goat Anti-Human Chordin-like 2/CHRDL2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2448) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Chordin-like 2/CHRDL2

CHRDL2, also known as breast tumor novel factor 1 (BNF-1), is a secreted BMP antagonist implicated in chondrocyte development.

Entrez Gene IDs
25884 (Human); 69121 (Mouse)
Alternate Names
BNF-1; Chordin like 2; Chordin-like 2; chordin-like protein 2; CHRDL2

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human Chordin-like 2/CHRDL2 Antibody

Average Rating: 4 (Based on 2 Reviews)

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Human Chordin-like 2/CHRDL2 Antibody
By Anonymous on 03/11/2019
Application: Microarrays Sample Tested: EDTA Plasma Species: Human

Human Chordin-like 2/CHRDL2 Antibody
By Anonymous on 10/31/2018
Application: MiAr Sample Tested: EDTA Plasma Species: Human