Human CHRM5 Antibody

Catalog # Availability Size / Price Qty
MAB10323-100
MAB10323-SP
Human CHRM5 Antibody in Flow Cytometry
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Human CHRM5 Antibody Summary

Species Reactivity
Human
Specificity
Detects human CHRM5 in diect ELISAs.
Source
Monoclonal Mouse IgG2A Clone # 590123
Purification
Protein A or G purified from ascites
Immunogen
NS0 mouse myeloma cell line transfected with human  CHRM5
Met1-Pro532
Accession # P08912
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of CHRM5 in HEK293 Human Cell Line Transfected with Human CHRM5 and eGFP by Flow Cytometry. View Larger

Detection of CHRM5 in HEK293 Human Cell Line Transfected with Human CHRM5 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) human CHRM5 or (B) irrelevant protein and eGFP was stained with Mouse Anti-Human CHRM5 Monoclonal Antibody (Catalog # MAB10323) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB003). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CHRM5

Muscarinic receptors are widely distributed throughout the body and control distinct functions according to location and subtype (M1 - M5). They are predominantly expressed in the parasympathetic nervous system where they exert both inhibitory and excitatory effects. Stimulation of CHRM5 is known to increase cyclic AMP levels. The clinical implications of CHRM5 are unknown but it may be linked to schizophrenia.

Long Name
Cholinergic Receptor Muscarinic 5
Entrez Gene IDs
1133 (Human); 213788 (Mouse); 53949 (Rat)
Alternate Names
cholinergic receptor, muscarinic 5; CHRM5; HM5; M5; M5R; mAChR M5; MGC41838; muscarinic acetylcholine receptor M5

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

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Isotype Controls

Mouse IgG2A Isotype Control

Reconstitution Buffers

Secondary Antibodies

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