Human Collagen XXIII  alpha 1 Antibody

Catalog # Availability Size / Price Qty
MAB4165
MAB4165-SP
Human Collagen XXIII  alpha 1 Antibody in Data
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Product Details
Citations (1)
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Human Collagen XXIII  alpha 1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Collagen XXIII alpha 1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, 50% cross-reactivity with recombinant human Collagen XXV alpha 1 is observed.
Source
Monoclonal Mouse IgG2B Clone # 468642
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Collagen XXIII alpha 1
Glu111-Lys540
Accession # NP_775736
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Immunohistochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human Collagen XXIII a1 antibody by Western Blot. View Larger

Detection of Human Collagen XXIII alpha 1 by Western Blot. Western blot shows lysates of NCI-H460 human large cell lung carcinoma cell line. PVDF Membrane was probed with 2 µg/mL of Human Collagen XXIII a1 Monoclonal Antibody (Catalog # MAB4165) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for Collagen XXIII a1 at approximately 75 and 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry Collagen XXIII a1 antibody in Human Adenocarcinoma by Immunohistochemistry (IHC-P). View Larger

Collagen XXIII alpha 1 in Human Adenocarcinoma. Collagen XXIII a1 was detected in immersion fixed paraffin-embedded sections of human adenocarcinoma using Human Collagen XXIII a1 Monoclonal Antibody (Catalog # MAB4165) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Collagen XXIII alpha 1

Collagen XXIII alpha 1 (COL23A1) is an epithelial cell type 2 transmembrane glycoprotein with multiple collagen like domains in its extracellular region. Intracellular furin mediated cleavage of the 68 kDa molecule generates a 58 kDa shed fragment that forms disulfide linked trimers. An alternately spliced isoform consists of nearly 300 amino acids of the membrane proximal extracellular domain (ECD), flanked by substituted sequences. COL23A1 is upregulated in many transformed cell lines and serves as an indicator for tumor aggressiveness in prostate cancer. Within the ECD, human COL23A1 shares 89% aa sequence identity with mouse and rat COL23A1.

Entrez Gene IDs
91522 (Human); 237759 (Mouse); 353303 (Rat)
Alternate Names
COL23A1; collagen alpha-1(XXIII) chain; Collagen XXIII alpha 1; collagen, type XXIII, alpha 1; DKFZp434K0621; procollagen, type XXIII, alpha 1

Product Datasheets

Citation for Human Collagen XXIII  alpha 1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. A role for collagen XXIII in cancer cell adhesion, anchorage-independence and metastasis.
    Authors: Spivey KA, Chung I, Banyard J
    Oncogene, 2012;31(18):2362-72.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

FAQs

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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