Human Common gamma Chain/IL‑2 R gamma APC‑conjugated Antibody

Name: Human Common gamma Chain/IL‑2 R gamma APC‑conjugated Antibody
Brand: R&D Systems
SKU: FAB2842A
Price: 439 USD
Availability: InStock

R&D Systems | Catalog # FAB2842A

Submit a review

Citations (1)

Product Documents

View Available Conjugates

100% Guarantee

Product Specifications
Scientific Data
Applications
Spectra Viewer
Flow Cytometry
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Mouse IgG_2B Clone # 633162

View all Common gamma Chain/IL-2 R gamma Primary Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf21-derived recombinant human Common gamma Chain/IL-2 gamma
Leu23-Asn254
Accession # P31785

Specificity

Detects human Common gamma Chain/IL-2 R gamma in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) IL-2 R alpha, rhIL-2 R beta, rhIL-15 R alpha, recombinant mouse IL-2 R gamma, or recombinant canine IL-2 R alpha is observed. In Western blots, approximately 80% cross-reactivity with rhIL-2 R beta, 50% cross-reactivity with rhIL-15 R alpha and recombinant mouse IL-2 R alpha, and no cross-reactivity withrhIL-2 R alpha is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2B

Scientific Data Images for Human Common gamma Chain/IL‑2 R gamma APC‑conjugated Antibody

Detection of Common gamma Chain/IL‑2 R gamma in Human Blood Lymphocytes by Flow Cytometry.

Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD3e PE-conjugated Monoclonal Antibody (Catalog # FAB100P) and either (A) Mouse Anti-Human Common gamma Chain/IL-2 R gamma APC-conjugated Monoclonal Antibody (Catalog # FAB2842A) or (B) Mouse IgG_2BAllophycocyanin Isotype Control (Catalog # IC0041A). View our protocol for Staining Membrane-associated Proteins.

Applications for Human Common gamma Chain/IL‑2 R gamma APC‑conjugated Antibody

Application

Recommended Usage

Flow Cytometry

10 µL/10⁶ cells
Sample: Human peripheral blood lymphocytes

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
Spillover Popups - Visualize the spectra of individual fluorochromes
Antigen Density Selector - Match fluorochrome brightness with antigen density

Build Your Panel Now

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: Common gamma Chain/IL-2 R gamma

The gamma chain of the high affinity functional human IL-2 receptor complex belongs to the hematopoietin receptor family. IL-2 R gamma is a 369 amino acid protein consisting of a 22 aa signal sequence, a 232 aa extracellular domain, a 29 aa transmembrane domain and an 86 aa cytoplasmic domain. Although IL-2 R gamma by itself does not bind IL‑2 with any appreciable affinity, it is required for IL-2 receptor signaling. Besides IL-2, the gamma chain has been shown to be a component of the functional receptor complexes for IL-4, IL-7, IL-9 and IL-15. IL-2 R gamma has been designated the common gamma chain ( gamma _c). The site of molecular defects in X-linked SCID (severe combined immunodeficiency) has been mapped to the IL-2 R gamma gene.