Detects human Common gamma Chain/IL-2 R gamma in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) IL-2 R alpha, rhIL-2 R beta, rhIL-15 R alpha, recombinant mouse IL-2 R gamma, or recombinant canine IL-2 R alpha is observed. In Western blots, approximately 80% cross-reactivity with recombinant human IL-2 R beta, 50% cross-reactivity with rhIL-15 R alpha and recombinant mouse IL-2 R alpha, and no cross-reactivity with rhIL-2 R alpha is observed.
Monoclonal Mouse IgG2B Clone # 633162
Protein A or G purified from hybridoma culture supernatant
S. frugiperda insect ovarian cell line Sf21-derived recombinant human Common gamma Chain/IL-2 gamma Leu23-Asn254 Accession # P31785
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
<0.10 EU per 1 μg of the antibody by the LAL method.
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Measured by its ability to neutralize IL‑2-induced proliferation in the MO7e human megakaryocytic leukemic cell line. Avanzi, G. et al. (1988) Br. J. Haematol. 69:359. The Neutralization Dose (ND50) is typically 0.08-0.4 µg/mL in the presence of 200 ng/mL Recombinant Human IL‑2.
Please Note: Optimal dilutions should be determined by each laboratory for each application.
are available in the Technical Information section on our website.
Detection of Common gamma Chain/IL‑2 R gamma in Human PBMC by Flow Cytometry.
Human peripheral blood lymphocytes were stained with Mouse Anti-Human Common gamma Chain/IL‑2 R gamma Monoclonal Antibody (Catalog # MAB2842, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0101B).
Cell Proliferation Induced by IL‑2 and Neutralization by Human Common gamma Chain/ IL‑2 R gamma Antibody.
Recombinant Human IL‑2 (Catalog # 202-IL) stimulates proliferation in the MO7e human megakaryocytic leukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑2 (200 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human Common gamma Chain/IL‑2 R gamma Monoclonal Antibody (Catalog # MAB2842). The ND50 is typically 0.08-0.4 µg/mL.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Common gamma Chain/IL-2 R gamma
The gamma chain of the high affinity functional human IL-2 receptor complex belongs to the hematopoietin receptor family. IL-2 R gamma is a 369 amino acid protein consisting of a 22 aa signal sequence, a 232 aa extracellular domain, a 29 aa transmembrane domain and an 86 aa cytoplasmic domain. Although IL-2 R gamma by itself does not bind IL‑2 with any appreciable affinity, it is required for IL-2 receptor signaling. Besides IL-2, the gamma chain has been shown to be a component of the functional receptor complexes for IL-4, IL-7, IL-9 and IL-15. IL-2 R gamma has been designated the common gamma chain ( gamma c). The site of molecular defects in X-linked SCID (severe combined immunodeficiency) has been mapped to the IL-2 R gamma gene.
Minami, Y. et al. (1993) Annu. Rev. Immunol. 11:245.
Noguchi, M. et al. (1993) Science 262:1877.
IL-2 Receptor gamma
Entrez Gene IDs:
3561 (Human); 16186 (Mouse)
CD132 antigen; CD132; CIDX; combined immunodeficiency, X-linked; common cytokine receptor gamma chain; Common gamma Chain; common gamma-chain; cytokine receptor common subunit gamma; gamma(c); IL-2 R gamma; IL-2 receptor subunit gamma; IL2R gamma; IL-2R subunit gamma; IL2RG; IL-2RG; IMD4; interleukin 2 receptor, gamma; Interleukin-2 receptor subunit gamma; P64; SCIDX; SCIDX1; severe combined immunodeficiency
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
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