Human CTGF/CCN2 C-Terminus Antibody

Catalog # Availability Size / Price Qty
AF660
AF660-SP
Human CTGF/CCN2 C-Terminus Antibody in Immunohistochemistry
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Product Details
Citations (3)
FAQs
Supplemental Products
Reviews (3)

Human CTGF/CCN2 C-Terminus Antibody Summary

Species Reactivity
Human
Specificity
Detects human CTGF/CCN2 C-Terminus in direct ELISAs. In direct ELISAs, approximately 15% cross‑reactivity with recombinant rat CTGF is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human CTGF/CCN2 C-Terminus
Glu247-Ala349
Accession # P29279
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Immunohistochemistry CTGF/CCN2 in Human Kidney. View Larger

CTGF/CCN2 in Human Kidney. CTGF/CCN2 was detected in immersion fixed paraffin-embedded sections of human kidney array using Goat Anti-Human CTGF/CCN2 C‑Terminus Antigen Affinity-purified Polyclonal Antibody (Catalog # AF660) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunohistochemistry CTGF/CCN2 in Human Pancreas. View Larger

CTGF/CCN2 in Human Pancreas. CTGF/CCN2 was detected in immersion fixed paraffin-embedded sections of human pancreas array using Goat Anti-Human CTGF/CCN2 C-Terminus Antigen Affinity-purified Polyclonal Antibody (Catalog # AF660) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CTGF/CCN2

CTGF belongs to the CCN (CYR61/CTGF/NOV) family of secreted proteins that share a common multimodular organization. Each protein contains an IGF-binding protein domain, a von Willebrand factor type C domain, a thrombospondin type I domain, and a cysteine knot domain. The multimodular CTGF has the ability to bind multiple ligands and has numerous effects on development, differentation, and disease. The C-terminal cysteine knot motif contains the heparin and low density lipoprotein receptor (LDLR) binding sites that contributes to the adhesive and mitogenic properties of CTGF.

Long Name
Connective Tissue Growth Factor
Entrez Gene IDs
1490 (Human); 14219 (Mouse); 64032 (Rat)
Alternate Names
CCN2; CCN2IGFBP-8; connective tissue growth factor; CTGF; CTGRP; Fisp12; HCS24; Hypertrophic chondrocyte-specific protein 24; IBP-8; IGF-binding protein 8; IGFBP-8; IGFBP8CCN family member 2; Insulin-like growth factor-binding protein 8; MGC102839; NOV2

Product Datasheets

Citations for Human CTGF/CCN2 C-Terminus Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Automated Analysis and Classification of Histological Tissue Features by Multi-Dimensional Microscopic Molecular Profiling.
    Authors: Riordan D, Varma S, West R, Brown P
    PLoS ONE, 2015;10(7):e0128975.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  2. SPARC mediates early extracellular matrix remodeling following myocardial infarction.
    Authors: McCurdy S, Dai Q, Zhang J, Zamilpa R, Ramirez T, Dayah T, Nguyen N, Jin Y, Bradshaw A, Lindsey M
    Am J Physiol Heart Circ Physiol, 2011;301(2):H497-505.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  3. Connective tissue growth factor and its correlation to other growth factors in experimental granulation tissue.
    Authors: Inkinen K, Wolff H, Lindroos P, Ahonen J
    Connect. Tissue Res., 2003;44(1):19-29.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC-P

FAQs

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human CTGF/CCN2 C-Terminus Antibody

Average Rating: 3.7 (Based on 3 Reviews)

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Human CTGF/CCN2 C-Terminus Antibody
By Anonymous on 11/10/2018
Application: ELISA Sample Tested: Serum,Plasma Species: Human

Human CTGF/CCN2 C-Terminus Antibody
By Anonymous on 11/09/2018
Application: MiAr Sample Tested: EDTA Plasma Species: Human

Human CTGF/CCN2 C-Terminus Antibody
By Anonymous on 11/07/2018
Application: Microarrays Sample Tested: EDTA Plasma Species: Human