Human CXCL14/BRAK Antibody

Catalog # Availability Size / Price Qty
AF866
AF866-SP
Product Details
Citations (2)
FAQs
Supplemental Products
Reviews

Human CXCL14/BRAK Antibody Summary

Species Reactivity
Human
Specificity
Detects human CXCL14/BRAK in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross‑reactivity with recombinant mouse CXCL14/BRAK is observed and less than 1% cross‑reactivity with rh6Ckine, rhBLC/BCA-1, rhCTACK, rhENA-78, rhEotaxin, rhFractalkine, rhGCP-2, rhGRO alpha, rhGRO beta, rhGRO gamma, rhHCC-1, rhI-309, rhIL-8, rhIP-10, rhI-TAC, rhLeukotactin-1, rhLymphotactin, rhMCP-1, rhMDC, rhMIP-1 alpha, rhMIP-1 beta, rmMIP-2, rhMIP-3 alpha, rhMIP-3 beta, rhMPIF-1, rhNAP-2, rhPARC, rhPF4, rhRANTES, rhTARC and rhTECK is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human CXCL14/BRAK (R&D Systems, Catalog # 866-CX)
Ser23-Glu99
Accession # O95715
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human CXCL14/BRAK (Catalog # 866-CX)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CXCL14/BRAK

CXCL14/BRAK (breast and kidney-expressed chemokine), also named MIP-2 gamma, KEC (kidney-expressed chemokine), and BMAC (B cell and monocyte-activating chemokine), is a member of CXC chemokine superfamily (1 - 5). The deduced 99 amino acid (aa) residue precursor has a 22 aa putative signal peptide that is cleaved to produce the 77 aa mature protein. Mature human and mouse CXCL14 differ by only 2 residues. Human CXCL14 shares approximately 30% aa sequence identity with MIP-2 alpha (GRO beta ) as well as MIP-2 beta  (GRO gamma ). The gene for CXCL14 has been mapped human chromosome 5q31. Unlike the MIP-2 chemokines, CXCL14 lacks the ELR domain preceding the CXC motif. CXCL14 transcripts are constitutively expressed at high levels in the basal layer of epidermal keratinocytes and dermal fibroblasts of skin tissues as well as lamina propria cells in normal intestinal tissues. CXCL14 has been shown to be a highly selective chemoattractant for monocytes that have been treated with prostaglandin E2 or forskolin, agents that activate adenylate cyclase. CXCL14 has been proposed to be important for regulating the trafficking of macrophage precursor to regions in skin and mucosal tissues that support their development. Consistent with this hypothesis, macrophages were frequently found to co-localize with CXCL14-producing cells in the dermis and lamina propria.

References
  1. Hromas, R. et al. (1999) Biochem. Biophys. Res. Commun. 255:703.
  2. Cao, X. et al. (2000) J. Immunol. 165:2588.
  3. Kurth, I. et al. (2001) J. Exp. Med. 194:855.
  4. Frederick, M.J. et al. (2000) Am. J. Pathol. 156:1937.
  5. Sleeman, M.A. et al. (2000) Int. Immunol. 12:677.
Entrez Gene IDs
9547 (Human); 57266 (Mouse)
Alternate Names
BMAC; Bolekine; BRAK; BRAKKEC; chemokine (C-X-C motif) ligand 14; Chemokine BRAK; CXCL14; CXC-X3; member 14 (BRAK); MIP-2 gamma; MIP2G; MIP-2G; NJACKec; SCYB14MGC10687; Small-inducible cytokine B14

Product Datasheets

Citations for Human CXCL14/BRAK Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Fascaplysin Exerts Anti-Cancer Effects through the Downregulation of Survivin and HIF-1? and Inhibition of VEGFR2 and TRKA
    Authors: TI Oh, YM Lee, TJ Nam, YS Ko, S Mah, J Kim, Y Kim, RH Reddy, YJ Kim, S Hong, JH Lim
    Int J Mol Sci, 2017;18(10):.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: WB
  2. Expression of the NF-kappaB targets BCL2 and BIRC5/Survivin characterizes small B-cell and aggressive B-cell lymphomas, respectively.
    Authors: Tracey L, Perez-Rosado A, Artiga MJ, Camacho FI, Rodriguez A, Martinez N, Ruiz-Ballesteros E, Mollejo M, Martinez B, Cuadros M, Garcia JF, Lawler M, Piris MA
    J. Pathol., 2005;206(2):123-34.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

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Secondary Antibodies

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