Human CXCL14/BRAK Antibody

(2 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human CXCL14/BRAK in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross‑reactivity with recombinant mouse CXCL14/BRAK is observed and less than 1% cross‑reactivity with rh6Ckine, rhBLC/BCA-1, rhCTACK, rhENA-78, rhEotaxin, rhFractalkine, rhGCP-2, rhGRO alpha, rhGRO beta, rhGRO gamma, rhHCC-1, rhI-309, rhIL-8, rhIP-10, rhI-TAC, rhLeukotactin-1, rhLymphotactin, rhMCP-1, rhMDC, rhMIP-1 alpha, rhMIP-1 beta, rmMIP-2, rhMIP-3 alpha, rhMIP-3 beta, rhMPIF-1, rhNAP-2, rhPARC, rhPF4, rhRANTES, rhTARC and rhTECK is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human CXCL14/BRAK (R&D Systems, Catalog # 866-CX)
    Ser23-Glu99
    Accession # O95715
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Human CXCL14/BRAK (Catalog # 866-CX)
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CXCL14/BRAK

CXCL14/BRAK (breast and kidney-expressed chemokine), also named MIP-2 gamma, KEC (kidney-expressed chemokine), and BMAC (B cell and monocyte-activating chemokine), is a member of CXC chemokine superfamily (1 - 5). The deduced 99 amino acid (aa) residue precursor has a 22 aa putative signal peptide that is cleaved to produce the 77 aa mature protein. Mature human and mouse CXCL14 differ by only 2 residues. Human CXCL14 shares approximately 30% aa sequence identity with MIP-2 alpha (GRO beta ) as well as MIP-2 beta  (GRO gamma ). The gene for CXCL14 has been mapped human chromosome 5q31. Unlike the MIP-2 chemokines, CXCL14 lacks the ELR domain preceding the CXC motif. CXCL14 transcripts are constitutively expressed at high levels in the basal layer of epidermal keratinocytes and dermal fibroblasts of skin tissues as well as lamina propria cells in normal intestinal tissues. CXCL14 has been shown to be a highly selective chemoattractant for monocytes that have been treated with prostaglandin E2 or forskolin, agents that activate adenylate cyclase. CXCL14 has been proposed to be important for regulating the trafficking of macrophage precursor to regions in skin and mucosal tissues that support their development. Consistent with this hypothesis, macrophages were frequently found to co-localize with CXCL14-producing cells in the dermis and lamina propria.

  • References:
    1. Hromas, R. et al. (1999) Biochem. Biophys. Res. Commun. 255:703.
    2. Cao, X. et al. (2000) J. Immunol. 165:2588.
    3. Kurth, I. et al. (2001) J. Exp. Med. 194:855.
    4. Frederick, M.J. et al. (2000) Am. J. Pathol. 156:1937.
    5. Sleeman, M.A. et al. (2000) Int. Immunol. 12:677.
  • Entrez Gene IDs:
    9547 (Human); 57266 (Mouse)
  • Alternate Names:
    BMAC; Bolekine; BRAK; BRAKKEC; chemokine (C-X-C motif) ligand 14; Chemokine BRAK; CXCL14; CXC-X3; member 14 (BRAK); MIP-2 gamma; MIP2G; MIP-2G; NJACKec; SCYB14MGC10687; Small-inducible cytokine B14
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Species
Applications
Sample Type
  1. The cytokine gene CXCL14 restricts human trophoblast cell invasion by suppressing gelatinase activity.
    Authors: Kuang H, Chen Q, Zhang Y, Zhang L, Peng H, Ning L, Cao Y, Duan E
    Endocrinology, 2009;150(12):5596-605.
    Species: Human
    Sample Type: Whole Cells
    Application: Blocking
  2. Expression of the NF-kappaB targets BCL2 and BIRC5/Survivin characterizes small B-cell and aggressive B-cell lymphomas, respectively.
    Authors: Tracey L, Perez-Rosado A, Artiga MJ, Camacho FI, Rodriguez A, Martinez N, Ruiz-Ballesteros E, Mollejo M, Martinez B, Cuadros M, Garcia JF, Lawler M, Piris MA
    J. Pathol., 2005;206(2):123-34.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
Isotype Controls
Description Application Cat# Citations Images  

Normal Goat IgG Control

Ctrl AB-108-C 191  
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Secondary Antibodies
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Goat IgG HRP-conjugated Antibody

WB, Simple Western HAF109 19  
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Goat IgG HRP-conjugated Antibody

WB HAF017 15  
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Goat IgG (H+L) PE-conjugated Antibody

Flow F0107 4  
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Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL001 8
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Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL003 5
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Goat IgG (H+L) APC-conjugated Antibody

Flow F0108 6  
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Goat IgG Horseradish Peroxidase-conjugated Antibody

WB HAF019 6  
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Donkey Anti-Goat IgG Antibody

WB AF109 6
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Donkey Anti-Goat IgG Biotinylated Antibody

WB BAF109 3
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Goat IgG VisUCyte HRP Polymer

IHC VC004  
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Goat IgG (H+L) Fluorescein-conjugated Antibody

Flow F0109 2  
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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL002
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Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

WB, ELISA R-401-C-ABS
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Rabbit Anti-Goat IgG Biotinylated Antibody

WB BAF017
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Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

Flow F0124
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