Human CXCR1/IL-8 RA Antibody Summary
Accession # AAA59159
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of CXCR1/IL‑8 RA in Human Blood Granulocytes by Flow Cytometry. Human peripheral blood granulocytes were stained with Mouse Anti-HumanCXCR1/IL‑8 RA Monoclonal Antibody (Catalog # MAB330R, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by PE-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.
CXCR1/IL‑8 RA in Human Stomach Cancer Tissue. CXCR1/IL‑8 RA was detected in immersion fixed paraffin-embedded sections of human stomach cancer tissue using Mouse Anti-Human CXCR1/IL‑8 RA Monoclonal Antibody (Catalog # MAB330R) at 0.5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and cell surfaces in cancer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Chemotaxis Induced by CXCL8/IL-8 and Neutralization by Human CXCR1/IL-8 RA Antibody. Recombinant Human CXCL8/IL-8 (Catalog # 208-IL) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CXCL8/IL-8 (1 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CXCR1/IL-8 RA Monoclonal Antibody (Catalog # MAB330R). The ND50 is typically 0.4-2.0 µg/mL.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CXCR1/IL-8 RA
The human C-X-C chemokine IL-8 is a potent neutrophil chemotactic and activating factor. Two distinct G protein-linked cell surface receptors, known as IL-8 RA (type I or CXCR1) and IL-8 RB (type II or CXCR2), can interact with the IL-8 molecule. These two receptors share 77% amino acid homology. CXCR1 expression has been documented on neutrophils, monocytes, and a small population of T cells.
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