Detection of CXCR7/RDC‑1 in MCF‑7 Human Cell Line by Flow Cytometry. MCF‑7 human breast cancer cell line was stained with Human CXCR7/ RDC‑1 Monoclonal Antibody (Catalog # MAB42273, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0101B).
CXCR7/RDC‑1 in Human Breast Cancer Tissue in a Mouse Model. CXCR7/RDC‑1 was detected in perfusion fixed paraffin-embedded sections of human breast cancer tissue in a mouse model using Human CXCR7/RDC‑1 Monoclonal Antibody (Catalog # MAB42273) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
The G protein-coupled receptor, RDC1, belongs to a subgroup of chemokine receptors and has been designated CXCR7. CXCR7 can bind with high-affinity to CXCL12/SDF-1 and CXCL11/I‑TAC. It is also a co-receptor for several HIV and SIV strains. In their N-termini and extracellular loops 1, 2, and 3, human and mouse CXCR7 share 84%, 100%, 96%, and 86% amino acid sequence identity, respectively. Reports of mRNA levels and/or protein expression (as assessed using anti-CXCR7, clone 9C4) (J. Biol. Chem. 2005, 280(42):35760, J. Immunol. 2006, 176(4):2197) indicate that CXCR7 occurs on a wide variety of tissues and cells including monocytes, B cells, T cells and mature dendritic cells. In contrast, based on ligand binding analysis and receptor level (as assessed using anti-CXCR7, clone 11G8), surface expression of CXCR7 was reported to be restricted to tumor cells, activated endothelial cells, fetal liver cells, and few other cell types (J. Exp. Med. 2006, 203(9):2201). The basis of these inconsistent observations is not known but may be attributed to cell context and the use of different antibodies that may recognize different epitopes.
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
Inhibition of CXCR7 extends survival following irradiation of brain tumours in mice and rats. Authors: Walters, M J, Ebsworth, K, Berahovich, R D, Penfold, M E T, Liu, S-C, Al Omran, R, Kioi, M, Chernikova, S B, Tseng, D, Mulkearns-Hubert, E E, Sinyuk, M, Ransohoff, R M, Lathia, J D, Karamchandani, J, Kohrt, H E K, Zhang, P, Powers, J P, Jaen, J C, Schall, T J, Merchant, M, Recht, L, Brown, J M Br J Cancer, 2014;110(5):1179-88. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded
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