Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Rat, Transgenic Mouse

Applications

Validated:

Immunohistochemistry, Flow Cytometry, CyTOF-ready

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Neutralization, Flow Cytometry, Immunocytochemistry, Immunoprecipitation, Bioassay, Cell Selection, Functional Assay, IHC-, Inhibition

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 11G8
View all CXCR7/RDC-1 Primary Antibodies »

Product Specifications

Immunogen

Human CXCR7 encoding plasmid
Accession # P25106

Specificity

Detects human CXCR7/RDC-1 in flow cytometry of five distinct human CXCR7 transfectants, but not their respective parental lines.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human CXCR7/RDC‑1 Antibody

Detection of CXCR7/RDC‑1 antibody in MCF‑7 Human Cell Line antibody by Flow Cytometry.

Detection of CXCR7/RDC‑1 in MCF‑7 Human Cell Line by Flow Cytometry.

MCF-7 human breast cancer cell line was stained with Mouse Anti-Human CXCR7/RDC-1 Monoclonal Antibody (Catalog # MAB42273, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0101B).

CXCR7/RDC-1 antibody in Human Squamous Cell Carcinoma by Immunohistochemistry (IHC-P).

CXCR7/RDC‑1 in Human Squamous Cell Carcinoma.

CXCR7/RDC-1 was detected in immersion fixed paraffin-embedded sections of human squamous cell carcinoma using Mouse Anti-Human CXCR7/RDC-1 Monoclonal Antibody (Catalog # MAB42273) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and plasma membrane in cancer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Detection of Human CXCR7/RDC-1 by Functional

Detection of Human CXCR7/RDC-1 by Functional

CXCL12 induces CXCR4/CXCR7 and cell polarization in HUVECs. HUVECs were cultured on plastic Petri dishes (pretreated with PDL) with BSA stripes (the control group) and BSA plus CXCL12 stripes (CXCL12 group) for 5 min. HUVECs were fixed and stained with antibodies against CXCR4 (purple), CXCR7 (blue) and F-Actin (red). The micro stripes of fluorescein-conjugated BSA (A) or CXCL12 (H) are shown. CXCR4 (B), CXCR7 (C) and F-Actin (D) in HUVECs cultured on BSA stripes stayed in the resting state, while the polarization of CXCR4 (arrow: I), CXCR7 (arrow: J) and F-Actin (arrow: K) was observed in HUVECs cultured on CXCL12 stripes for 5 min. Moreover, the HUVECs in the BSA control group showed no morphological changes (E–G). The CXCL12 group HUVECs shows polarization towards the CXCL12 stripe (L–N). Scale bar: 10 μm. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28811579), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human CXCR7/RDC-1 by Functional

Detection of Human CXCR7/RDC-1 by Functional

CXCL12 induces CXCR4/CXCR7 and cell polarization in HUVECs. HUVECs were cultured on plastic Petri dishes (pretreated with PDL) with BSA stripes (the control group) and BSA plus CXCL12 stripes (CXCL12 group) for 5 min. HUVECs were fixed and stained with antibodies against CXCR4 (purple), CXCR7 (blue) and F-Actin (red). The micro stripes of fluorescein-conjugated BSA (A) or CXCL12 (H) are shown. CXCR4 (B), CXCR7 (C) and F-Actin (D) in HUVECs cultured on BSA stripes stayed in the resting state, while the polarization of CXCR4 (arrow: I), CXCR7 (arrow: J) and F-Actin (arrow: K) was observed in HUVECs cultured on CXCL12 stripes for 5 min. Moreover, the HUVECs in the BSA control group showed no morphological changes (E–G). The CXCL12 group HUVECs shows polarization towards the CXCL12 stripe (L–N). Scale bar: 10 μm. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28811579), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of CXCR7/RDC-1 by Western Blot

Detection of CXCR7/RDC-1 by Western Blot

Impact of CXCR7 over-expression on the E2-dependent and -independent growth of MCF-7 cells.MCF-7 cells were transiently transfected with either a control expression vector or one containing the human CXCR7 open reading frame. (A) Total protein extracts were prepared 48 h after transfection, and a Western blot analysis was performed to confirm CXCR7 over-expression. (B) Transfected cells were cultured in the presence of EtOH (−) or 10−8 M E2 (+) for seven days. E2-dependent and E2-independent cell growth rates were then evaluated by cell count of three independent experiments (n = 3). The results are expressed as a percentage of the relative cell number obtained from control cells treated with E2 (considered as 100%). Significant differences (p<0.05) between transfected cells in the absence of E2 are indicated by an asterisk and between transfected cells in the presence of E2 by a sharp symbol. (C) A proposed model for the involvement of the CXCL12 signaling axis in E2-dependent and -independent cell growth is shown. The binding of CXCL12 to CXCR4 and CXCR7 leads to the stimulation of cell growth through diverse pathways [28]. CXCR7 can also modulate CXCL12 availability by removing the chemokine from the extracellular space (left panel). Estrogens could stimulate cell growth by favoring the activation of CXCL12 through CXCR4 and reducing the expression of CXCR7 (right panel). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/21695171), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human CXCR7/RDC‑1 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: MCF‑7 human breast cancer cell line

Immunohistochemistry

8-25 µg/mL
Sample: Perfusion fixed paraffin-embedded sections of nude mice injected with human breast cancer tissue cells and immersion fixed paraffin-embedded sections of human squamous cell carcinoma

Reviewed Applications

Read 2 reviews rated 4 using MAB42273 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CXCR7/RDC-1

The G protein-coupled receptor, RDC1, belongs to a subgroup of chemokine receptors and has been designated CXCR7. CXCR7 can bind with high-affinity to CXCL12/SDF-1 and CXCL11/I‑TAC. It is also a co-receptor for several HIV and SIV strains. In their N-termini and extracellular loops 1, 2, and 3, human and mouse CXCR7 share 84%, 100%, 96%, and 86% amino acid sequence identity, respectively. Reports of mRNA levels and/or protein expression (as assessed using anti-CXCR7, clone 9C4) (J. Biol. Chem. 2005, 280(42):35760, J. Immunol. 2006, 176(4):2197) indicate that CXCR7 occurs on a wide variety of tissues and cells including monocytes, B cells, T cells and mature dendritic cells. In contrast, based on ligand binding analysis and receptor level (as assessed using anti-CXCR7, clone 11G8), surface expression of CXCR7 was reported to be restricted to tumor cells, activated endothelial cells, fetal liver cells, and few other cell types (J. Exp. Med. 2006, 203(9):2201). The basis of these inconsistent observations is not known but may be attributed to cell context and the use of different antibodies that may recognize different epitopes.

Alternate Names

ACKR3, CMKOR1, CXCR7, GPR159, RDC-1

Entrez Gene IDs

57007 (Human); 12778 (Mouse); 84348 (Rat)

Gene Symbol

ACKR3

UniProt

P25106

Additional CXCR7/RDC-1 Products

Product Documents for Human CXCR7/RDC‑1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human CXCR7/RDC‑1 Antibody

For research use only

Citations for Human CXCR7/RDC‑1 Antibody

Customer Reviews for Human CXCR7/RDC‑1 Antibody (2)

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Customer Images

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  • Human CXCR7/RDC-1 Antibody
    Name: Kirsten Hattermann-Koch
    Application: Immunohistochemistry-Frozen
    Sample Tested: Mouse brain
    Species: Mouse
    Verified Customer | Posted 03/11/2019
    10 µm Mouse brain cryosections stained with CXCR7 antibody (1:100) and Alexa Fluor 555 donkey anti mouse IgG. Antibody works quite well with mouse tissue, blood vessels are positive in the brain as they should be, for example.
    Human CXCR7/RDC‑1 Antibody MAB42273
  • Name: Anonymous
    Application: ELISA
    Sample Tested: See PMID 22457824
    Species: Human
    Verified Customer | Posted 02/20/2015

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Protocols

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FAQs for Human CXCR7/RDC‑1 Antibody

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    • A: MAB42273 and MAB42273R have a kappa light chain. 
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