Human CXCR7/RDC-1 Antibody

(10 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human CXCR7/RDC-1 in flow cytometry of five distinct human CXCR7 transfectants, but not their respective parental lines.
  • Source
    Monoclonal Mouse IgG1 Clone # 11G8
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Human CXCR7 encoding plasmid
    Accession # P25106
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    0.25 µg/106 cells
    See below
  • Immunohistochemistry
    8-25 µg/mL
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of CXCR7/RDC‑1 in MCF‑7 Human Cell Line by Flow Cytometry. MCF‑7 human breast cancer cell line was stained with Human CXCR7/
RDC‑1 Monoclonal Antibody (Catalog # MAB42273, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0101B).
Immunohistochemistry
CXCR7/RDC‑1 in Human Breast Cancer Tissue in a Mouse Model. CXCR7/RDC‑1 was detected in perfusion fixed paraffin-embedded sections of human breast cancer tissue in a mouse model using Human CXCR7/RDC‑1 Monoclonal Antibody (Catalog # MAB42273) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CXCR7/RDC-1

The G protein-coupled receptor, RDC1, belongs to a subgroup of chemokine receptors and has been designated CXCR7. CXCR7 can bind with high-affinity to CXCL12/SDF-1 and CXCL11/I‑TAC. It is also a co-receptor for several HIV and SIV strains. In their N-termini and extracellular loops 1, 2, and 3, human and mouse CXCR7 share 84%, 100%, 96%, and 86% amino acid sequence identity, respectively. Reports of mRNA levels and/or protein expression (as assessed using anti-CXCR7, clone 9C4) (J. Biol. Chem. 2005, 280(42):35760, J. Immunol. 2006, 176(4):2197) indicate that CXCR7 occurs on a wide variety of tissues and cells including monocytes, B cells, T cells and mature dendritic cells. In contrast, based on ligand binding analysis and receptor level (as assessed using anti-CXCR7, clone 11G8), surface expression of CXCR7 was reported to be restricted to tumor cells, activated endothelial cells, fetal liver cells, and few other cell types (J. Exp. Med. 2006, 203(9):2201). The basis of these inconsistent observations is not known but may be attributed to cell context and the use of different antibodies that may recognize different epitopes.

  • Entrez Gene IDs:
    57007 (Human); 12778 (Mouse); 84348 (Rat)
  • Alternate Names:
    ACKR3; chemokine (C-X-C motif) receptor 7; Chemokine orphan receptor 1CMKOR1C-X-C chemokine receptor type 7; CMKOR1; CXCR7; CXC-R7; CXCR-7; G protein-coupled receptor; GPR159; GPR159G-protein coupled receptor 159; RDC-1; RDC1G-protein coupled receptor RDC1 homolog
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. CXCL12 enhances angiogenesis through CXCR7 activation in human umbilical vein endothelial cells
    Authors: M Zhang, L Qiu, Y Zhang, D Xu, JC Zheng, L Jiang
    Sci Rep, 2017;7(1):8289.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  2. SDF-1alpha is a novel autocrine activator of platelets operating through its receptor CXCR4.
    Authors: Walsh T, Harper M, Poole A
    Cell Signal, 2015;27(1):37-46.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  3. The CXCR4/CXCR7/CXCL12 Axis Is Involved in a Secondary but Complex Control of Neuroblastoma Metastatic Cell Homing.
    Authors: Muhlethaler-Mottet A, Liberman J, Ascencao K, Flahaut M, Balmas Bourloud K, Yan P, Jauquier N, Gross N, Joseph J
    PLoS ONE, 2015;10(5):e0125616.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  4. Divergent angiocrine signals from vascular niche balance liver regeneration and fibrosis.
    Authors: Ding B, Cao Z, Lis R, Nolan D, Guo P, Simons M, Penfold M, Shido K, Rabbany S, Rafii S
    Nature, 2014;505(7481):97-102.
    Species: Human
    Sample Type: Cell Lysates
    Application: IP
  5. Inhibition of CXCR7 extends survival following irradiation of brain tumours in mice and rats.
    Authors: Walters, M J, Ebsworth, K, Berahovich, R D, Penfold, M E T, Liu, S-C, Al Omran, R, Kioi, M, Chernikova, S B, Tseng, D, Mulkearns-Hubert, E E, Sinyuk, M, Ransohoff, R M, Lathia, J D, Karamchandani, J, Kohrt, H E K, Zhang, P, Powers, J P, Jaen, J C, Schall, T J, Merchant, M, Recht, L, Brown, J M
    Br J Cancer, 2014;110(5):1179-88.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  6. COUP-TFI modifies CXCL12 and CXCR4 expression by activating EGF signaling and stimulates breast cancer cell migration.
    Authors: Boudot A, Kerdivel G, Lecomte S, Flouriot G, Desille M, Godey F, Leveque J, Tas P, Le Drean Y, Pakdel F
    BMC Cancer, 2014;14(0):407.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  7. Chemokine receptor CXCR7 is a functional receptor for CXCL12 in brain endothelial cells.
    Authors: Liu Y, Carson-Walter E, Walter K
    PLoS ONE, 2014;9(8):e103938.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  8. Ubiquitination of CXCR7 controls receptor trafficking.
    Authors: Canals M, Scholten DJ, de Munnik S, Han MK, Smit MJ, Leurs R
    PLoS ONE, 2012;7(3):e34192.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  9. Involvement of the CXCR7/CXCR4/CXCL12 axis in the malignant progression of human neuroblastoma.
    Authors: Liberman J, Sartelet H, Flahaut M, Muhlethaler-Mottet A, Coulon A, Nyalendo C, Vassal G, Joseph J, Gross N
    PLoS ONE, 2012;7(8):e43665.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  10. CXCR7 functions as a scavenger for CXCL12 and CXCL11.
    Authors: Naumann U, Cameroni E, Pruenster M, Mahabaleshwar H, Raz E, Zerwes HG, Rot A, Thelen M
    PLoS ONE, 2010;5(2):e9175.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
Cell and Tissue Staining Kits
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Anti-Mouse HRP-DAB Cell & Tissue Staining Kit

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Immunohistochemistry Reagents
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Isotype Controls
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Mouse IgG1 Isotype Control

Ctrl MAB002 138  
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Mouse IgG1 Isotype Control

Ctrl MAB002R 1
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Secondary Antibodies
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Mouse IgG HRP-conjugated Antibody

WB HAF007 26  
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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0102B 10  
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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow, IHC, ICC NL007 9
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
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Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 8
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2  
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 5
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Mouse IgG Antibody

WB AF007 4
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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