Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 722407
View all Cyclin A1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Cyclin A1
Lys51-Arg220
Accession # P78396

Specificity

Detects human Cyclin A1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human Cyclin A2, B1, B2, C, D1, D2, D3, E1, E2, or recombinant mouse Cyclin A1 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Cyclin A1 Antibody

Detection of Human Cyclin A1 antibody by Western Blot.

Detection of Human Cyclin A1 by Western Blot.

Western blot shows lysates of THP-1 human acute monocytic leukemia cell line, HeLa human cervical epithelial carcinoma cell line, NTera-2 human testicular embryonic carcinoma cell line, MCF-7 human breast cancer cell line, HEK293 human embryonic kidney cell line, and human testis tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Cyclin A1 Monoclonal Antibody (Catalog # MAB7046) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Cyclin A1 at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Cyclin A1 antibody in Human Testis by Immunohistochemistry (IHC-P).

Cyclin A1 in Human Testis.

Cyclin A1 was detected in immersion fixed paraffin-embedded sections of human testis using Mouse Anti-Human Cyclin A1 Monoclonal Antibody (Catalog # MAB7046) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei of spermatocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human Cyclin A1 Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human testis

Western Blot

1 µg/mL
Sample: THP‑1 human acute monocytic leukemia cell line, HeLa human cervical epithelial carcinoma cell line, NTera‑2 human testicular embryonic carcinoma cell line, MCF‑7 human breast cancer cell line, HEK293 human embryonic kidney cell line, and human testis tissue

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Cyclin A1

Cyclin A1, also known as CCNA1, is a 64 kDa protein that associates with the kinase Cdk2. This complex phosphorylates multiple substrates involved in regulation of cell cycle progression, apoptosis, double strand DNA break repair, and mRNA splicing. Cyclin A1 activity promotes G2/M phase transition in spermatocyte meiosis and G1/S transition in somatic cells. Cyclin A1 is upregulated in myeloid leukemia, testicular germ cell tumors, and several carcinomas. It contains two cyclin box folds (aa 243-318 and 340-427). Over aa 51-220, human Cyclin A1 shares approximately 75% aa identity with mouse and rat Cyclin A1.

Alternate Names

CCNA1

Entrez Gene IDs

8900 (Human); 12427 (Mouse); 295052 (Rat)

Gene Symbol

CCNA1

UniProt

P78396

Additional Cyclin A1 Products

Product Documents for Human Cyclin A1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Cyclin A1 Antibody

For research use only

Citations for Human Cyclin A1 Antibody

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Protocols

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