Human Cyclin A1 Antibody

Catalog # Availability Size / Price Qty
MAB7046
MAB7046-SP
Detection of Human Cyclin A1 by Western Blot.
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Product Details
Citations (1)
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Human Cyclin A1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Cyclin A1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human Cyclin A2, B1, B2, C, D1, D2, D3, E1, E2, or recombinant mouse Cyclin A1 is observed.
Source
Monoclonal Mouse IgG1 Clone # 722407
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Cyclin A1
Lys51-Arg220
Accession # P78396
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunohistochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human Cyclin A1 by Western Blot. View Larger

Detection of Human Cyclin A1 by Western Blot. Western blot shows lysates of THP-1 human acute monocytic leukemia cell line, HeLa human cervical epithelial carcinoma cell line, NTera-2 human testicular embryonic carcinoma cell line, MCF-7 human breast cancer cell line, HEK293 human embryonic kidney cell line, and human testis tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Cyclin A1 Monoclonal Antibody (Catalog # MAB7046) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Cyclin A1 at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry Cyclin A1 in Human Testis. View Larger

Cyclin A1 in Human Testis. Cyclin A1 was detected in immersion fixed paraffin-embedded sections of human testis using Mouse Anti-Human Cyclin A1 Monoclonal Antibody (Catalog # MAB7046) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei of spermatocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cyclin A1

Cyclin A1, also known as CCNA1, is a 64 kDa protein that associates with the kinase Cdk2. This complex phosphorylates multiple substrates involved in regulation of cell cycle progression, apoptosis, double strand DNA break repair, and mRNA splicing. Cyclin A1 activity promotes G2/M phase transition in spermatocyte meiosis and G1/S transition in somatic cells. Cyclin A1 is upregulated in myeloid leukemia, testicular germ cell tumors, and several carcinomas. It contains two cyclin box folds (aa 243-318 and 340-427). Over aa 51-220, human Cyclin A1 shares approximately 75% aa identity with mouse and rat Cyclin A1.

Entrez Gene IDs
8900 (Human); 12427 (Mouse); 295052 (Rat)
Alternate Names
CCNA1; Cyclin A1; cyclin-A1

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Citation for Human Cyclin A1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Androgen Receptor-Mediated Growth Suppression of HPr-1AR and PC3-Lenti-AR Prostate Epithelial Cells.
    Authors: Kim Y, Chen C, Bolton E
    PLoS ONE, 2015;10(9):e0138286.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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