Human Cystatin A Antibody

Catalog #: MAB1407
1 Citation Datasheet / COA / SDS

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MAB1407
MAB1407-SP

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Product Details

Citations (1)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Cystatin A Antibody Summary

Species Reactivity

Human

Specificity

Detects human Cystatin A in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) Cystatins B, C, D, E/M, F, S, SA, rhFetuin A, rhFetuin B, rhHPRG, rhKininogen, or rhKininostatin is observed.

Source

Monoclonal Mouse IgG_2A Clone # 224705

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human Cystatin A
Ile2-Phe98
Accession # P01040

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human Cystatin A (Catalog # 1407-PI)

Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Recombinant Human Cystatin A (Catalog # 1407-PI), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cystatin A

Cystatin A, also called stefin A, Cystatin AS or keratolinin, is a member of family 1 of the cystatin superfamily (1, 2). Like Cystatin B, it is an intracellular inhibitor regulating the activities of cysteine proteases of the papain family such as cathepsins B, H and L (3). For example, immunohistochemical analysis of Cystatin A and cathepsin L is a useful indicator for malignancy in human epidermal keratinocytes (4). The ratio of cathepsin B and Cystatin A can be used in the differential diagnosis and treatment of patients with prostate carcinoma (5). The human Cystatin A consists of 98 amino acids (1).

References

Kartasova, T. et al. (1987) Nucleic Acids Res. 15:5945.
Abrahamson, M. (1994) Methods Enzymol. 244:685.
Jenko, S. et al. (2003) J. Mol. Biol. 326:875.
Palungwachira, P. et al. (2002) J. Dermatol. 29:573.
Sinha, A.A. et al. (2002) Cancer 94:3141.

Entrez Gene IDs

1475 (Human)

Alternate Names

AREI; CSTA; cystatin A (stefin A); Cystatin A; cystatin AS; cystatin-A; Stefin A; STF1; STF1Cystatin-AS; STFA; STFAStefin-A

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Cysteine Proteases and Regulators

Citation for Human Cystatin A Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Cystatin B as a tissue and urinary biomarker of bladder cancer recurrence and disease progression.
Authors: Feldman AS, Banyard J, Wu CL, McDougal WS, Zetter BR
Clin. Cancer Res., 2009-02-01;15(3):1024-31.
Species: Human
Sample Types: Urine
Applications: Western Blot