Detection of Human DC-LAMP by Western Blot. Western blot shows lysates of in vitro differentiated human myeloid mature dendritic cells. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human DC-LAMP Monoclonal Antibody (Catalog # MAB4087) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for DC-LAMP at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
DC-LAMP (dendritic cell lysosome-associated membrane protein; also CD208 and LAMP3) is a member of the LAMP family of proteins. Mature human DC-LAMP is a 389 amino acid (aa) type I transmembrane protein. It can be variably glycosylated, and apparent sizes from 54kDa to 90 kDa have been reported. It has a 254 aa luminal N-terminus and a short 14 aa cytoplasmic tail. The molecule is found in type II pneumocytes, interdigitating DC, and various tumors. It may play a role in normal lysosome and endosome function. Over aa 21‑381, human DC-LAMP shares 72% and 55% aa sequence identity with dog and mouse DC‑LAMP, respectively.
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