Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 530217
View all DC-LAMP Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human DC-LAMP
Asp21-Thr381
Accession # EAW78338

Specificity

Detects human DC-LAMP in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human LAMP is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human DC‑LAMP Antibody

Detection of Human DC-LAMP antibody by Western Blot.

Detection of Human DC-LAMP by Western Blot.

Western blot shows lysates ofin vitrodifferentiated human myeloid mature dendritic cells. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human DC-LAMP Monoclonal Antibody (Catalog # MAB4087) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for DC-LAMP at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human DC‑LAMP Antibody

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: In vitro differentiated human myeloid mature dendritic cells

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: DC-LAMP

DC-LAMP (dendritic cell lysosome-associated membrane protein; also CD208 and LAMP3) is a  member of the LAMP family of proteins. Mature human DC-LAMP is a 389 amino acid (aa) type I transmembrane protein. It can be variably glycosylated, and apparent sizes from 54kDa to 90 kDa have been reported. It has a 254 aa luminal N-terminus and a short 14 aa cytoplasmic tail. The molecule is found in type II pneumocytes, interdigitating DC, and various tumors. It may play a role in normal lysosome and endosome function. Over aa 21‑381, human DC-LAMP shares 72% and 55% aa sequence identity with dog and mouse DC‑LAMP, respectively.

Long Name

Dendritic Cell-lysosome-associated Membrane Glycoprotein

Alternate Names

CD208, DCLAMP, LAMP3, TSC403

Entrez Gene IDs

27074 (Human); 239739 (Mouse); 303801 (Rat)

Gene Symbol

LAMP3

UniProt

EAW78338

Additional DC-LAMP Products

Product Documents for Human DC‑LAMP Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human DC‑LAMP Antibody

For research use only

Related Research Areas

Cancer Biomarkers
Lysosomal Enzymes

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