|Detection of Human DEC2 by Western Blot. Western blot shows lysates of human brain tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human DEC2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5226) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for DEC2 at approximately 50-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.|
DEC-2 (Differentially expressed in chondrocytes protein 2; also bHLHB3, Sharp-1 and bHLHe41) is a 54-56 kDa Class B member of the bHLH superfamily of proteins. It utilizes both DNA binding and protein-protein interaction to mediate E-box transcriptional repression. Physiologically, DEC-2 is proposed to be a regulator of the sleep cycle, particularly during periods of sleep deprivation. It also interacts with HIF-1 alpha neutralizing its activation of the VEGF gene during hypoxia. Human DEC-2 is 482 amino acids (aa) in length. It contains a DNA binding motif (aa 45-57), an HLH region (aa 58-100), an Orange domain that may impart specificity to protein-protein interactions (aa 131-166) and a Gly/Ala-rich region (aa 297-431). DEC-2 forms homodimers, and heterodimers with MyoD and E-proteins. There is one potential alternate start site at Met280. Over aa 101‑287, there is less than 60% aa identity between human and mouse DEC-2.
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.
The document you requested is not available online. Please enter the Catalog Number and Lot Number below to have a document emailed to you at the address provided