Detection of Human DEC2 by Western Blot.
Western blot shows lysates of human brain tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human DEC2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5226) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for DEC2 at approximately 50-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
DEC-2 (Differentially expressed in chondrocytes protein 2; also bHLHB3, Sharp-1 and bHLHe41) is a 54-56 kDa Class B member of the bHLH superfamily of proteins. It utilizes both DNA binding and protein-protein interaction to mediate E-box transcriptional repression. Physiologically, DEC-2 is proposed to be a regulator of the sleep cycle, particularly during periods of sleep deprivation. It also interacts with HIF-1 alpha neutralizing its activation of the VEGF gene during hypoxia. Human DEC-2 is 482 amino acids (aa) in length. It contains a DNA binding motif (aa 45-57), an HLH region (aa 58-100), an Orange domain that may impart specificity to protein-protein interactions (aa 131-166) and a Gly/Ala-rich region (aa 297-431). DEC-2 forms homodimers, and heterodimers with MyoD and E-proteins. There is one potential alternate start site at Met280. Over aa 101‑287, there is less than 60% aa identity between human and mouse DEC-2.
Differentially expressed in chondrocytes protein 2
Entrez Gene IDs:
79365 (Human); 79362 (Mouse); 117095 (Rat)
basic helix-loop-helix domain containing, class B, 3; basic helix-loop-helix family, member e41; BHLHB3; BHLHB3class E basic helix-loop-helix protein 41; BHLHE41; Class B basic helix-loop-helix protein 3; DEC2; DEC2Enhancer-of-split and hairy-related protein 1; Differentially expressed in chondrocytes protein 2; SHARP-1; SHARP1hDEC2
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