Human DEC2 Antibody

Catalog # Availability Size / Price Qty
Detection of Human DEC2 by Western Blot.
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Product Details
Supplemental Products

Human DEC2 Antibody Summary

Species Reactivity
Detects human DEC2 direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human DEC1 is observed.
Polyclonal Goat IgG
Antigen Affinity-purified
E. coli-derived recombinant human DEC2
Accession # Q9C0J9
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human DEC2 antibody by Western Blot. View Larger

Detection of Human DEC2 by Western Blot. Western blot shows lysates of human brain tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human DEC2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5226) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for DEC2 at approximately 50-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DEC2

DEC-2 (Differentially expressed in chondrocytes protein 2; also bHLHB3, Sharp-1 and bHLHe41) is a 54-56 kDa Class B member of the bHLH superfamily of proteins. It utilizes both DNA binding and protein-protein interaction to mediate E-box transcriptional repression. Physiologically, DEC-2 is proposed to be a regulator of the sleep cycle, particularly during periods of sleep deprivation. It also interacts with HIF-1 alpha neutralizing its activation of the VEGF gene during hypoxia. Human DEC-2 is 482 amino acids (aa) in length. It contains a DNA binding motif (aa 45-57), an HLH region (aa 58-100), an Orange domain that may impart specificity to protein-protein interactions (aa 131-166) and a Gly/Ala-rich region (aa 297-431). DEC-2 forms homodimers, and heterodimers with MyoD and E-proteins. There is one potential alternate start site at Met280. Over aa 101‑287, there is less than 60% aa identity between human and mouse DEC-2.

Long Name
Differentially expressed in chondrocytes protein 2
Entrez Gene IDs
79365 (Human); 79362 (Mouse); 117095 (Rat)
Alternate Names
basic helix-loop-helix domain containing, class B, 3; basic helix-loop-helix family, member e41; BHLHB3; BHLHB3class E basic helix-loop-helix protein 41; BHLHE41; Class B basic helix-loop-helix protein 3; DEC2; DEC2Enhancer-of-split and hairy-related protein 1; Differentially expressed in chondrocytes protein 2; SHARP-1; SHARP1hDEC2

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Goat IgG (H+L) Antibody


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