hh Human Decorin Antibody MAB143-100: R&D Systems

Human Decorin Antibody

(7 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human Decorin in direct ELISAs and Western blots. In direct ELISAs, this antibody does not cross-react with recombinant mouse Decorin.
  • Source
    Monoclonal Mouse IgG1 Clone # 115402
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Decorin
    Gly17-Lys172
    Accession # NP_598013.1
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Immunohistochemistry
    8-25 µg/mL
    Immersion fixed paraffin-embedded sections of human breast
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human Decorin by Western Blot. Western blot shows lysates of human uterus tissue and human heart tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Decorin Monoclonal Antibody (Catalog # MAB143) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Decorin at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Decorin

Decorin is a small secreted chondroitin/dermatan sulfate proteoglycan in the family of small leucine-rich proteoglycans (SLRPs). SLRP family members are characterized by N-terminal and C-terminal cysteine-rich regions which flank the central region containing 10-12 tandem leucine-rich repeats (LRR) (1, 2). The human Decorin cDNA encodes a 359 amino acid (aa) precursor that includes a 16 aa signal sequence and a 14 aa propeptide. The 329 aa mature protein contains twelve LRR. Alternate splicing generates five isoforms with variable length deletions (3). Mature human and mouse Decorin share 80% aa sequence identity. In Decorin, serine 34 in the N-terminal domain is O-glycosylated. Naturally occurring Decorin proteoglycan has a molecular mass of approximately 100 kDa, and the deglycosylated Decorin core protein has a mass of approximately 40 kDa. Decorin binds to fibronectin, TGF-beta, and type I and type II collagens. The binding of Decorin to various molecules was reported to be mediated via the core protein. Decorin has been implicated in matrix assembly and has also been reported to suppress the growth of various tumor cell lines by activating the epidermal growth factor receptor.

  • References:
    1. Naito, Z. (2005) J. Nippon Med. Sch. 72:137.
    2. Matsushima, N. et al. (2005) Cell. Mol. Life Sci. 62:2771.
    3. Danielson, K. et al. (1993) Genomics 15:146.
  • Entrez Gene IDs:
    1634 (Human); 13179 (Mouse)
  • Alternate Names:
    Bone proteoglycan II; CSCD; DCN; decorin proteoglycan; Decorin; dermatan sulphate proteoglycans II; DSPG2; PG40; PGII; PG-II; PGS2; PG-S2; proteoglycan core protein; SLRR1B; SLRR1BPGS2; small leucine-rich protein 1B
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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Species
Applications
Sample Type
  1. Dermatan sulfate is a player in the transglutaminase 2 interaction network
    Authors: G Wisowski, EM Ko?ma, T Bielecki, A Pude?ko, K Olczyk
    PLoS ONE, 2017;12(2):e0172263.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: IP
  2. Proteomics of thyroid tumours provides new insights into their molecular composition and changes associated with malignancy
    Authors: J Martínez-A, R Clifton-Bl, MP Molloy
    Sci Rep, 2016;6(0):23660.
    Species: Human
    Sample Type: Tissue Homogenates
    Application: WB
  3. Possible dual role of decorin in abdominal aortic aneurysm.
    Authors: Ueda, Koshiro, Yoshimura, Koichi, Yamashita, Osamu, Harada, Takasuke, Morikage, Noriyasu, Hamano, Kimikazu
    PLoS ONE, 2015;10(3):e0120689.
    Species: Human
    Sample Type: Tissue Homogenates
    Application: WB
  4. Defective Proteolytic Processing of Fibrillar Procollagens and Prodecorin Due to Biallelic BMP1 Mutations Results in a Severe, Progressive Form of Osteogenesis Imperfecta.
    Authors: Syx D, Guillemyn B, Symoens S, Sousa A, Medeira A, Whiteford M, Hermanns-Le T, Coucke P, De Paepe A, Malfait F
    J Bone Miner Res, 2015;30(8):1445-56.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: WB
  5. Faulty initiation of proteoglycan synthesis causes cardiac and joint defects.
    Authors: Baasanjav S, Al-Gazali L, Hashiguchi T, Mizumoto S, Fischer B, Horn D, Seelow D, Ali BR, Aziz SA, Langer R, Saleh AA, Becker C, Nurnberg G, Cantagrel V, Gleeson JG, Gomez D, Michel JB, Stricker S, Lindner TH, Nurnberg P, Sugahara K, Mundlos S, Hoffmann K
    Am. J. Hum. Genet., 2011;89(1):15-27.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: WB
  6. Lack of CXC chemokine receptor 3 signaling leads to hypertrophic and hypercellular scarring.
    Authors: Yates CC, Krishna P, Whaley D, Bodnar R, Turner T, Wells A
    Am. J. Pathol., 2010;176(4):1743-55.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  7. Role of decorin in the antimyeloma effects of osteoblasts.
    Authors: Li X, Pennisi A, Yaccoby S
    Blood, 2008;112(1):159-68.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
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