Human Desmoglein-1 Antibody
Human Desmoglein-1 Antibody Summary
Glu50-His545
Accession # Q02413
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Desmoglein‑1 in Human Skin. Desmoglein-1 was detected in immersion fixed paraffin-embedded sections of human skin using Goat Anti-Human Desmoglein-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF944) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Desmoglein-1
Desmoglein-1 is one of three members of the desmoglein subfamily of calcium-dependent cadherin cell adhesion molecules. Together with desmocollins, another subfamily within the cadherin superfamily, the desmoglein isoforms form the adhesive components of desmosomes, the cell-cell adhesive structures that are found in epithelial cells. Human Desmoglein-1 is a type I transmembrane glycoprotein of 1049 amino acid (aa) residues with a 23 aa signal peptide and a 26 aa propeptide. It differs from other classic cadherins by having four instead of five cadherin repeat domains in its extracellular region, and a much larger cytoplasmic region containing five desmoglein repeat domains which share homology with the cadherin repeats. Instead of having the HAV adhesion motif found in type I cadherins, Desmoglein-1 has R/YAL as the adhesion motif on its amino-terminal cadherin repeat. The cytoplasmic tail of Desmoglein-1 interacts with desmoplakins, plakoglobin and plakophilins. In turn, these proteins link the Desmoglein-1 with the intermediate filaments. Desmoglein-1 has been shown to be important in establishing cell-cell adhesion and function in the epidermis. In the autoimmune skin disease pemphigus foliaceus, autoantibodies to Desmoglein-1 can cause the loss of keratinocyte adhesion resulting in blisters.
- Nollet, R. et al. (2000) J. Mol. Biol. 299:551.
- Elias, P. et al. (2001) J. Cell Biol. 153:243.
Product Datasheets
Citations for Human Desmoglein-1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 2
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Desmoglein 1 regulates invadopodia by suppressing EGFR/Erk signaling in an Erbin-dependent manner
Authors: A Valenzuela, HE Burks, CR Arnette, A Yalamanchi, O Nekrasova, LM Godsel, KJ Green
Mol. Cancer Res., 2019;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Desmosomal cadherin association with Tctex-1 and cortactin-Arp2/3 drives perijunctional actin polymerization to promote keratinocyte delamination
Authors: O Nekrasova, RM Harmon, JA Broussard, JL Koetsier, LM Godsel, GN Fitz, ML Gardel, KJ Green
Nat Commun, 2018;9(1):1053.
Species: Human
Sample Types: Whole Cells
Applications: ICC
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