Human Desmoglein-3 Antibody

Catalog # Availability Size / Price Qty
AF1720
AF1720-SP
Product Details
Citations (1)
FAQs
Supplemental Products
Reviews

Human Desmoglein-3 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Desmoglein-3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross‑reactivity with recombinant human (rh) Desmoglein-1 and rhDesmoglein-2 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Desmoglein-3
Glu50-Arg615
Accession # P32926
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Desmoglein‑3 Fc Chimera (Catalog # 1720-DM)
Immunohistochemistry
5-15 µg/mL
Immersion fixed paraffin-embedded sections of human skin

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Desmoglein-3

Desmoglein-3, also known as pemphigus vulgaris antigen (PVA), is a 130 kDa transmembrane glycoprotein that belongs to the cadherin family of calcium dependent adhesion molecules (1‑3). Human Desmoglein-3 is synthesized with a 26 amino acid (aa) propeptide. The mature protein consists of a 566 aa extracellular domain (ECD) that contains four cadherin-like domains, a 25 aa transmembrane segment, and a 359 aa cytoplasmic domain (4). Within the ECD, human Desmoglein-3 shares 78% aa sequence identity with mouse and rat Desmoglein-3. It shares 44%, 47%, and 56% aa sequence identity with Desmoglein-1, -2, and -4 respectively. Desmoglein-3 is one of the principal components of desmosomes which form adhesive contacts between epithelial cells (1, 2). It is expressed in the basal and suprabasal layers of stratified epithelia in many tissues (4‑6). During apoptosis, Desmoglein-3 is cleaved by caspases, plus MMP-2, and MMP-9 at sites within the cytoplasmic and extracellular regions, resulting in shortened transmembrane forms and a soluble 75 kDa ECD fragment (7, 8). The downregulation of Desmoglein-3 in oral squamous cell carcinoma correlates with metastatic potential (9). Desmoglein-3 is the target of autoantibodies in pemphigus vulgaris, a blistering skin disorder with compromised inter-keratinocyte adhesion (6, 10). Binding of these antibodies triggers Desmoglein-3 internalization and degradation by keratinocytes (10).

References
  1. Kottke, M.D. et al. (2006) J. Cell Sci. 119:797.
  2. Garrod, D.R. et al. (2002) Mol. Membr. Biol. 19:81.
  3. Leckband, D. and A. Prakasam (2006) Annu. Rev. Biomed. Eng. 8:259.
  4. Amagai, M. et al. (1991) Cell 67:869.
  5. Silos, S.A. et al. (1996) J. Biol. Chem. 271:17504.
  6. Amagai, M. et al (1996) J. Invest. Dermatol. 106:351.
  7. Weiske, J. et al. (2001) J. Biol. Chem. 276:41175.
  8. Cirillo, N. et al. (2007) Oral Dis. 13:341.
  9. Wang, L. et al. (2007) Virchows Arch. Sept. 11 epub.
  10. Calkins, C.C. et al. (2006) J. Biol. Chem. 281:7623.
Entrez Gene IDs
1830 (Human); 13512 (Mouse)
Alternate Names
Cadherin family member 6; CDHF6; CDHF6130-kD pemphigus vulgaris antigen; desmoglein 3 (pemphigus vulgaris antigen); desmoglein 3; Desmoglein3; Desmoglein-3; DKFZp686P23184,130 kDa pemphigus vulgaris antigen; DSG3; pemphigus vulgaris antigen; PVA

Product Datasheets

Citation for Human Desmoglein-3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Critical Role of the Neonatal Fc Receptor (FcRn) in the Pathogenic Action of Antimitochondrial Autoantibodies Synergizing with Anti-desmoglein Autoantibodies in Pemphigus Vulgaris.
    Authors: Chen Y, Chernyavsky A, Webber R, Grando S, Wang P
    J Biol Chem, 2015;290(39):23826-37.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human Desmoglein-3 Antibody

There are currently no reviews for this product. Be the first to review Human Desmoglein-3 Antibody and earn rewards!

Have you used Human Desmoglein-3 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review