Detects human Desmoglein-4 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity
with recombinant human Desmoglein-1, -2, or -3 is observed and approximately
40% cross-reactivity with recombinant mouse Desmoglein-4 is observed.
Monoclonal Mouse IgG2A Clone # 683135
Protein A or G purified from hybridoma culture supernatant
Mouse myeloma cell line NS0-derived recombinant human Desmoglein-4 Glu24-Ala631 Accession # Q86SJ6
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Detection of Human Desmoglein-4 by Western Blot.
Western blot shows lysates of human skin tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Desmoglein-4 Monoclonal Antibody (Catalog # MAB6589) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Desmoglein-4 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Desmoglein-4 (DSG4) is a 110-130 kDa type I transmembrane glycoprotein in the cadherin family of calcium-dependent cell adhesion molecules. Desmogleins interact with transmembrane Desmocollins to form the adhesive components of desmosomes on epithelial cells. Desmoglein-4 contains four cadherin repeats in its extracellular region and two desmoglein repeat domains in its cytoplasmic region. Alternate splicing generates an additional isoform with a substitution in the cytoplasmic region. Desmoglein-4 is expressed on keratinocytes and trichocytes in the epidermis and hair follicles. It is required for hair follicle development, and DSG4 mutations are associated with autosomal recessive hypotrichosis. Within amino acids 24-631 (which includes the 26 aa propeptide and the ECD), human Desmoglein-4 shares 82% aa sequence identity with mouse and rat Desmoglein-4.
Entrez Gene IDs:
147409 (Human); 16769 (Mouse); 291754 (Rat)
Cad13; Cadherin family member 13; CDGF13; CDHF13; CDHF13CDH family member 13; desmoglein 4; Desmoglein4; Desmoglein-4; DSG4; LAHdesmoglein-4
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