|Dopamine D2 R/DRD2 in Human Brain. Dopamine D2 R/DRD2 was detected in immersion fixed paraffin-embedded sections of human brain (striatum) using Mouse Anti-Human Dopamine D2 R/DRD2 Monoclonal Antibody (Catalog # MAB9266) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell membranes and cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.|
Dopamine receptor D2 (DRD2) is localized to human striatum, motor cortex and neocortex. It is a highly conserved seven transmembrane receptor member of the G-protein coupled receptor 1 family, with three known isoforms. In the striatum, DRD2 suppresses voluntary activity in the striatopallidal pathway, and polymorphisms in this gene are associated with alcohol addiction, smoking behavior, schizophrenia, food addiction and post-traumatic stress disorder as well as myoclonus dystonia. DRD2 has been shown to heterodimerize with DDR4 and 5-HT2A receptors, and traffics between the plasma membrane and intracellular pools and this localization can be modulated by several drugs. DRD2 is localized to human striatum, motor cortex and neocortex.
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