Human Dopamine D2 R/DRD2 Antibody (960710)

Dopamine D2 R/DRD2 in Human Brain.

Dopamine D2 R/DRD2 was detected in immersion fixed paraffin-embedded sections of human brain (striatum) using Mouse Anti-Human Dopamine D2 R/DRD2 Monoclonal Antibody (Catalog # MAB9266) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell membranes and cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Human Dopamine D2R/DRD2 by Immunocytochemistry/ Immunofluorescence

Expression of MCT8 and OATP1C1 in D2-MSN (indirect pathway medium-sized spiny neurons) in human and macaque striatum. Representative confocal microscope compositions from double-stained sections for MCT8 (green) or OATP1C1 (green) and for the D2-MSN marker DRD2 (red) in the putamen. Merged images (right side) show the colocalization of the two signals. Coexpression of MCT8 and DRD2 is observed in human (A–C) and macaque (D–F) striatum. Coexpression of OATP1C1 and DRD2 is observed in human (G–I) and macaque (J–L) striatum. Counterstaining with DAPI (blue) shows nuclei of all cells. Note that in humans, the MCT8 signals are located mainly at the cell membrane, while in macaques, they are also located in the cytoplasm. D2-MSN: D2 receptor-expressing medium-sized spiny neurons; DRD2: Dopamine receptor type 2; Put: putamen. Scale bar = 50 μm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37298594), licensed under a CC-BY license. Not internally tested by R&D Systems.