Human Dopamine D5 R/DRD5 Antibody

    
Datasheet / CoA / SDS
Product Details
FAQs
Supplemental Products
Reviews
  • Species Reactivity
    Human
  • Specificity
    Detects HEK293 human embryonic kidney cell line transfected with human Dopamine D5 R/DRD5 by Flow Cytometry. Does not detect untransfected or irrelevant transfected HEK293 cells.
  • Source
    Monoclonal Mouse IgG2A Clone # 889022
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    NS0 mouse myeloma cell line transfected with human Dopamine D5 R/DRD5
    Met1-His477
    Accession # P21918
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Product Datasheets

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Certificate of Analysis Lookup

Certificate of Analysis Request Form

To download a Certificate of Analysis, please enter the catalog and lot numbers in the search box below.

Note: Certificate of Analysis not available for kit components.

Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    0.25 µg/106 cells
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Detection of Dopamine D5 R/DRD5 in HEK293 Human Cell Line Transfected with Human Dopamine D5 R/DRD5 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) human Dopamine D5 R/DRD5 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human Dopamine D5 R/DRD5 Monoclonal Antibody (Catalog # MAB82861) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB003).
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
    Reconstitution Buffer Available
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Dopamine D5 R/DRD5
DRD5, also known as Dopamine D5 Receptor has a higher affinity for neurotransmitter dopamine than DRD1 (1). DRD5 is expressed in neurons in many human brain regions, including cortex regions, hippocampus, choroid plexus, and brainstem (2). Polymorphisms in the DRD5 gene have been associated with Attention Deficit Hyperactivity Disorder (ADHD) (3), schizopherenia (4) and nicotine dependence (5). Dopamine receptors undergo endocytosis upon interaction with receptor agonists and by activation of Protein Kinase C (PKC) (6).
  • References:
    1. Sunahra, R. et al. (1991) Nature. 350:614.
    2. Weinshank, R. et al. (1991) J Biol Chem. 266:22427.
    3. Squassina, A. et al.  (2008) Neurosci Lett. Feb 13.
    4. Golimbet, V. et al.  (2008) Bull Exp Biol Med. Jan.
    5. Wei, J. et al.  (2012) Addict Behav. 37:622.
    6. Thompson, D. et al. (2011) Traffic. 12:644.
  • Long Name:
    Dopamine D5 Receptor
  • Entrez Gene IDs:
    1816 (Human); 13492 (Mouse); 25195 (Rat)
  • Alternate Names:
    D(1B) dopamine receptor; D(5) dopamine receptor; D1beta dopamine receptor; DBDR; Dopamine D5 R; Dopamine D5 receptor; Dopamine D5R; dopamine receptor D1B; dopamine receptor D5; DRD1B; DRD1BDBDR; DRD1L2; DRD1L2MGC10601; DRD5
Related Research Areas

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2A Isotype Control

Ctrl MAB003 35  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse IgG2A Isotype Control

Ctrl MAB0031 7  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse IgG2A Isotype Control

Ctrl MAB003R
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Reconstitution Buffers
Description Application Cat# Citations Images  

Reconstitution Buffer 1 (PBS)

RB01
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Secondary Antibodies
Description Application Cat# Citations Images  

Mouse IgG HRP-conjugated Antibody

WB HAF007 32  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0102B 12  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow , IHC , ICC NL007 10
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow , IHC , ICC NL008 8
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 4  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse IgG Antibody

WB AF007 4
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow , IHC , ICC NL009 5
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse IgG2A Antibody

WB , Flow MAB0033  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Rat Anti-Mouse IgG2A PE-conjugated Antibody

Flow F0129 1  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Rat Anti-Mouse IgG2A APC-conjugated Antibody

Flow F0130  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse IgG2A Antibody

Flow MAB0032  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Rat Anti-Mouse IgG2A PerCP-conjugated Antibody

Flow F0131  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Have you used Human Dopamine D5 R/DRD5 Antibody?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Order Details

Contact Us to Order
reload

Feedback

Customer Information
Calculators

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Monoclonal
Antibody
Panels For
Screening

Learn More
Monoclonal Antibodies
R&D Systems Guarantee