Detection of Dopamine D5 R/DRD5 in HEK293 Human Cell Line Transfected with Human Dopamine D5 R/DRD5 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) human Dopamine D5 R/DRD5 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human Dopamine D5 R/DRD5 Monoclonal Antibody (Catalog # MAB82861) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB003).
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Dopamine D5 R/DRD5
DRD5, also known as Dopamine D5 Receptor has a higher affinity for neurotransmitter dopamine than DRD1 (1). DRD5 is expressed in neurons in many human brain regions, including cortex regions, hippocampus, choroid plexus, and brainstem (2). Polymorphisms in the DRD5 gene have been associated with Attention Deficit Hyperactivity Disorder (ADHD) (3), schizopherenia (4) and nicotine dependence (5). Dopamine receptors undergo endocytosis upon interaction with receptor agonists and by activation of Protein Kinase C (PKC) (6).
Sunahra, R. et al. (1991) Nature. 350:614.
Weinshank, R. et al. (1991) J Biol Chem. 266:22427.
Squassina, A. et al. (2008) Neurosci Lett. Feb 13.
Golimbet, V. et al. (2008) Bull Exp Biol Med. Jan.
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.