Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 889022
View all Dopamine D5R/DRD5 Primary Antibodies »

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with human Dopamine D5 R/DRD5
Met1-His477
Accession # P21918

Specificity

Detects HEK293 human embryonic kidney cell line transfected with human Dopamine D5 R/DRD5 by Flow Cytometry. Does not detect untransfected or irrelevant transfected HEK293 cells.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human Dopamine D5R/DRD5 Antibody

Detection of Dopamine D5 R/DRD5 antibody in HEK293 Human Cell Line Transfected with Human Dopamine D5 R/DRD5 and eGFP antibody by Flow Cytometry.

Detection of Dopamine D5 R/DRD5 in HEK293 Human Cell Line Transfected with Human Dopamine D5 R/DRD5 and eGFP by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with either (A) human Dopamine D5 R/DRD5 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human Dopamine D5 R/DRD5 Monoclonal Antibody (Catalog # MAB82861) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB003).

Applications for Human Dopamine D5R/DRD5 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: HEK293 human embryonic kidney cell line transfected with human Dopamine D5 R/DRD5 and eGFP

Reviewed Applications

Read 2 reviews rated 4 using MAB82861 in the following applications:

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: Dopamine D5R/DRD5

DRD5, also known as Dopamine D5 Receptor has a higher affinity for neurotransmitter dopamine than DRD1 (1). DRD5 is expressed in neurons in many human brain regions, including cortex regions, hippocampus, choroid plexus, and brainstem (2). Polymorphisms in the DRD5 gene have been associated with Attention Deficit Hyperactivity Disorder (ADHD) (3), schizopherenia (4) and nicotine dependence (5). Dopamine receptors undergo endocytosis upon interaction with receptor agonists and by activation of Protein Kinase C (PKC) (6).

References

  1. Sunahra, R. et al. (1991) Nature. 350:614.
  2. Weinshank, R. et al. (1991) J Biol Chem. 266:22427.
  3. Squassina, A. et al.  (2008) Neurosci Lett. Feb 13.
  4. Golimbet, V. et al.  (2008) Bull Exp Biol Med. Jan.
  5. Wei, J. et al.  (2012) Addict Behav. 37:622.
  6. Thompson, D. et al. (2011) Traffic. 12:644.

Long Name

Dopamine D5 Receptor

Alternate Names

DBDR, Dopamine D5 R, DRD1B, DRD1L2, DRD5

Entrez Gene IDs

1816 (Human); 13492 (Mouse); 25195 (Rat)

Gene Symbol

DRD5

UniProt

P21918

Additional Dopamine D5R/DRD5 Products

Product Documents for Human Dopamine D5R/DRD5 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Dopamine D5R/DRD5 Antibody

For research use only

Customer Reviews for Human Dopamine D5R/DRD5 Antibody (2)

4 out of 5
2 Customer Ratings
5 Stars
0%
4 Stars
100%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used Human Dopamine D5R/DRD5 Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 2 of 2 reviews Showing All
Filter By:
Clear Filters X
  • Human Dopamine D5R/DRD5 Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Whole blood
    Species: Human
    Verified Customer | Posted 12/17/2020
    Whole blood was diluted with PBS, overlaid atop Ficoll, and centrifuged to separate the PBMC layer. The PBMC layer was washed twice with PBS and cells were aliquoted into centrifuge tubes for staining. Cells were counted and concentration was adjusted to 10,000 cells per uL. 100 uL of cells was aliquoted into each centrifuge tube for surface staining. 1uL of anti-DRD5 antibody was added to the 'primary + secondary' condition. Cells were incubated on ice for 30 mins protected from light. Following incubation, cells were washed twice with PBS and then fixed at room temperature for 30 mins protected from light. Cells were washed twice with permeabilization buffer and resuspended in 100 uL perm buffer for secondary staining. 1uL of secondary antibody (R&D systems, Cat#F0102B) was added to both the 'secondary only' and 'primary + secondary' condition. Cells were incubated at room temperature for 30 mins protected from light. Following 2x perm buffer washes, cells were resuspended in 500uL PBS for flow cytometry acquisition. 'Secondary only' condition (left) was used to determine the gate placement.'Primary + secondary' condition (right) shows successful flow cytometry staining for DRD5 in total PBMC fraction.
    Human Dopamine D5R/DRD5 Antibody MAB82861
  • Human Dopamine D5R/DRD5 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: 293T human embryonic kidney cell line and HEK293 human embryonic kidney cell line
    Species: Human
    Verified Customer | Posted 12/01/2020
    We transfected HEK cells with a DRD5 plasmid and stained them for DRD5 at a 1:1000 concentration. Secondary only and parental HEK cells were used as controls to confirm specific staining.
    Human Dopamine D5R/DRD5 Antibody MAB82861

There are no reviews that match your criteria.

Showing  1 - 2 of 2 reviews Showing All

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies