Western blot shows lysates of human liver tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human DPP9 Monoclonal Antibody (Catalog # MAB5419) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for DPP9 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
DPP9 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human DPP9 Monoclonal Antibody (Catalog # MAB5419) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Simple Western lane view shows lysates of human liver tissue, loaded at 0.5 mg/mL. A specific band was detected for DPP9 at approximately 101 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human DPP9 Monoclonal Antibody (Catalog # MAB5419). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
DPP9 is a member of the S9b family of serine peptidases (1, 2). It shares 19% amino acid identity with DPP4 and 58% amino acid identity with DPP8. It exhibits post‑proline dipeptidyl aminopeptidase activity, cleaving Xaa-Pro dipeptides from the N-terminus of oligo- and polypeptides (3). Unlike DPP4, DPP9 does not appear to be membrane bound and is localized exclusively in the cytoplasm (4). This family of proline-specific dipeptidyl peptidases has been implicated in a variety of diseases including type 2 diabetes, obesity and cancer, and has been a potential target for drug discovery (5, 6).
Olsen, C. and Wagtmann, N. (2002) Gene 299:185.
Qi, S.Y. et al. (2003) Biochem. J. 373:179.
Bjelke, J.R. et al. (2006) Biochem. J. 396:391.
Ajami, K. et al. (2004) Biochim. Biophys. Acta. 1679:18.
Rosenblum, J.S. and Kozarich, J.W. et al. (2003) Curr. Opin. Chem. Biol. 7:496.
Van der Veken, P. et al. (2007) Curr. Top. Med. Chem. 7:621.
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